Open Access

Fabrication of DNA micropatterns on the polycarbonate surface of compact discs

Nanoscale Research Letters20062:69

DOI: 10.1007/s11671-006-9032-6

Received: 24 October 2006

Accepted: 8 November 2006

Published: 8 December 2006

Abstract

DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as “anchor” to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.

Keywords

DNA Compact disc UV/ozone Hybridization

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Authors’ Affiliations

(1)
Center for Advanced Materials & Biotechnology, Research Institute of Tsinghua University in Shenzhen

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Copyright

© to the authors 2006