Open Access

Combing and self-assembly phenomena in dry films of Taxol-stabilized microtubules

  • FabriceOlivier Morin1Email author,
  • Franck Rose2,
  • Pascal Martin3,
  • Mehmet C Tarhan4,
  • Hideki Kawakatsu1 and
  • Hiroyuki Fujita1
Nanoscale Research Letters20072:135

DOI: 10.1007/s11671-007-9044-x

Received: 13 December 2006

Accepted: 4 February 2007

Published: 13 March 2007


Microtubules are filamentous proteins that act as a substrate for the translocation of motor proteins. As such, they may be envisioned as a scaffold for the self-assembly of functional materials and devices. Physisorption, self-assembly and combing are here investigated as a potential prelude to microtubule-templated self-assembly. Dense films of self-assembled microtubules were successfully produced, as well as patterns of both dendritic and non-dendritic bundles of microtubules. They are presented in the present paper and the mechanism of their formation is discussed.


Adsorption HOPG Microtubules Self-assembly


(See supplementary material 1)



F.O.M. and F.R. acknowledge the financial support of the Japan Society for the Promotion of Science (JSPS). P.M. acknowledges the financial support of the Centre National de la Recherche Scientifique (CNRS). F.O.M. and M.C.T. acknowledge prof. S. Takeuchi for permitting the use of ultra-centrifuges.

Authors’ Affiliations

LIMMS-CNRS/IIS, UMI 2820, University of Tokyo
Materials Sciences Division, L. Berkeley National Laboratory
L2MP, Institut Supérieur d’Electronique et du Numérique Maison des Technologies
IIS, University of Tokyo


  1. Hirokawa N, Takemura R: Nature Rev. Neurosc.. 1995, 6: 201–214. 10.1038/nrn1624View Article
  2. Fritzsche W, Köhler JM, Böhm KJ, Unger E, Wagner T, Kirsch R, Mertig M, Pompe W: Nanotechnology. 1999, 10: 331–335. 10.1088/0957-4484/10/3/317View Article
  3. Fritzsche W, Böhm KJ, Unger E, Köhler JM: Appl. Phys. Lett.. 1999, 75: 2854–2856. COI number [1:CAS:528:DyaK1MXmslOgtLk%3D] 10.1063/1.125172View Article
  4. Behrens S, Rahn K, Habicht W, Böhm KJ, Rösner H, Dinjus E, Unger E: Advanced Mat.. 2002, 14: 1621–1625. COI number [1:CAS:528:DC%2BD38XpsFOrtLk%3D] 10.1002/1521-4095(20021118)14:22<1621::AID-ADMA1621>3.0.CO;2-DView Article
  5. Behrens S, Habicht W, Wu J, Unger E: Surf. Interf. Anal 2006, 38: 1014–1018. COI number [1:CAS:528:DC%2BD28Xmt1ejsbc%3D] 10.1002/sia.2334View Article
  6. Jia L, Moorjani SG, Jackson TN, Hancock WO: Biomed. Microdev.. 2004, 6: 67–74. COI number [1:CAS:528:DC%2BD2cXms1equw%3D%3D] 10.1023/B:BMMD.0000013368.89455.8dView Article
  7. Hess H, Bachand GD, Vogel V: Chem. Eur. J.. 2004, 10: 2110–2116. COI number [1:CAS:528:DC%2BD2cXktVGgs7g%3D] 10.1002/chem.200305712View Article
  8. Yan H, Park SH, Finkelstein G, Reif JH, LaBean TH: Science. 2003, 301: 1882–1884. COI number [1:CAS:528:DC%2BD3sXnsFSgtr0%3D] 10.1126/science.1089389View Article
  9. Zheng J, Constantinou PE, Micheel C, Alivisatos AP, Kiehl RA, Seeman NC: Nano Lett.. 2006, 6: 1502–1504. COI number [1:CAS:528:DC%2BD28Xlt1yqur0%3D] 10.1021/nl060994cView Article
  10. Stracke R, Burghold J, Schacht HJ, Böhm KJ, Unger E: Nanotechnology. 2000, 11: 52–56. COI number [1:CAS:528:DC%2BD3cXltFKgsbo%3D] 10.1088/0957-4484/11/2/302View Article
  11. Yokokawa R, Takeuchi S, Kon T, Nishiura M, Sutoh K, Fujita H: Nano Lett.. 2004, 4: 2265–2270. COI number [1:CAS:528:DC%2BD2cXoslCqsLo%3D] 10.1021/nl048851iView Article
  12. Limberis L, Magda JJ, Stewart RJ: Nano Lett.. 2001, 1: 277–280. COI number [1:CAS:528:DC%2BD3MXjt1Ojsbc%3D] 10.1021/nl0155375View Article
  13. Yang Y, Deymier PA, Wang L, Guzman R, Hoying JB, McLaughlin HJ, Smith SD, Jongewaard IN: Biotech. Progress. 2006, 22: 303–312. 10.1021/bp050150jView Article
  14. Roos W, Ulmer J, Gräter S, Surrey T, Spatz JP: Nano Lett.. 2005, 5: 2630–2634. COI number [1:CAS:528:DC%2BD2MXht1SmtrfE] 10.1021/nl051865jView Article
  15. Muthukrishnan G, Roberts CA, Chen Y-C, Zahn JD, Hancock WO: Nano Lett.. 2004, 4: 2127–2132. COI number [1:CAS:528:DC%2BD2cXnvF2qs7Y%3D] 10.1021/nl048816bView Article
  16. Vinckier A, Heyvaert I, D’Hoore A, McKittrick T, Haesendonck CV, Engelborghs Y, Hellemans L: Ultramicroscopy. 1995, 57: 337–343. COI number [1:CAS:528:DyaK2MXksV2ntrk%3D] 10.1016/0304-3991(94)00194-RView Article
  17. de Pablo PJ, Schaap IAT, Schmidt CF: Nanotechnology. 2003, 14: 143–146. 10.1088/0957-4484/14/2/308View Article
  18. Vater W, Fritzsche W, Schaper A, Böhm KJ, Unger E, Jovin TM: J. Cell Sc.. 1995, 108: 1063–1069.
  19. Yang Y, Guzman R, Deymier PA, Umnov M, Hoying J, Raghavan S, Palusinski O, Zelinski BJJ: J. Nanosc. Nanotech.. 2005, 5: 2050–2056. COI number [1:CAS:528:DC%2BD2MXhtlWnu77K] 10.1166/jnn.2005.510View Article
  20. Fritzsche W, Böhm KJ, Unger E, Köhler JM: Nanotechnology. 1998, 9: 177–183. COI number [1:CAS:528:DyaK1cXntFajs7c%3D] 10.1088/0957-4484/9/3/006View Article
  21. Maillard M, Motte L, Pileni M-P: Advanced Mat.. 2001, 13: 200–204. COI number [1:CAS:528:DC%2BD3MXhs12qu7s%3D] 10.1002/1521-4095(200102)13:3<200::AID-ADMA200>3.0.CO;2-PView Article
  22. Rabani E, Reichman DR, Geissler PL, Brus LE: Nature. 2003, 426: 271–274. COI number [1:CAS:528:DC%2BD3sXptVOisb0%3D] 10.1038/nature02087View Article
  23. Ray MA, Kim H, Jia L: Langmuir. 2005, 21: 4786–4789. COI number [1:CAS:528:DC%2BD2MXjsFahur0%3D] 10.1021/la050165rView Article
  24. Tabony J, Glade N, Demongeot J, Papaseit C: Langmuir. 2002, 18: 7196–7207. COI number [1:CAS:528:DC%2BD38XmtVShtb0%3D] 10.1021/la0255875View Article
  25. Nogales E, Whittaker M, Milligan RA, Downing KH: Cell. 1999, 96: 79–88. COI number [1:CAS:528:DyaK1MXmslagtA%3D%3D] 10.1016/S0092-8674(00)80961-7View Article
  26. Lin Z, Granick S: J. Am. Chem. Soc.. 2005, 127: 2816–2817. COI number [1:CAS:528:DC%2BD2MXht1Orurw%3D] 10.1021/ja044792zView Article
  27. Hong SW, Xu J, Xia J, Lin Z, Qiu F, Yang Y: Chem. Mat.. 2005, 17: 6223–6226. COI number [1:CAS:528:DC%2BD2MXht1Smtr3E] 10.1021/cm052078qView Article
  28. Dalton AB, Ortiz-Acevedo A, Zorbas V, Brunner E, Sampson WM, Collins S, Razal JM, Miki Yoshida M, Baughman RH, Draper RK, Musselman IH, Jose-Yacaman M, Dieckmann GR: Adv. Funct. Mat.. 2004, 14: 1147–1151. COI number [1:CAS:528:DC%2BD2MXlvV2ktg%3D%3D] 10.1002/adfm.200400190View Article
  29. Yokokawa R, Yoshida Y, Takeuchi S, Kon T, Fujita H: Nanotechnology. 2006, 17: 289–294. COI number [1:CAS:528:DC%2BD28XitVSrs78%3D] 10.1088/0957-4484/17/1/049View Article
  30. Tang JX, Wong S, Tran PT, Janmey PA: Ber Bunsenges. Phys. Chem.. 1996, 100: 796–806. View Article
  31. Tang JX, Ito T, Tao T, Straub P, Janmey PA: Biochemistry. 1997, 36: 12600–12607. COI number [1:CAS:528:DyaK2sXmtFCks74%3D] 10.1021/bi9711386View Article
  32. Deegan RD, Bakajin O, Dupont TF, Huber G, Nagel SR, Witten TA: Nature. 1997, 389: 827–829. COI number [1:CAS:528:DyaK2sXmvFSktrY%3D] 10.1038/39827View Article
  33. Tarasevich YY: Phys. Uspekhi. 2004, 47: 717–728. COI number [1:CAS:528:DC%2BD2cXhtVKitbfO] 10.1070/PU2004v047n07ABEH001758View Article
  34. Needleman DJ, Ojeda-Lopez MA, Raviv U, Ewert K, Miller HP, Wilson L, Safinya CR: Proc. Nat. Acad. Sc. USA. 2004, 101: 16099–16103. COI number [1:CAS:528:DC%2BD2cXhtVWks7rJ] 10.1073/pnas.0406076101View Article
  35. Smalyukh II, Zribi OV, Butler JC, Lavrentovich OD, Wong GCL: Phys. Rev. Lett.. 2006, 96: 177801. 10.1103/PhysRevLett.96.177801View Article
  36. Zribi OV, Kyung H, Golestanian R, Liverpool TB, Wong GCL: Phys. Rev. E. 2006, 73: 031911. 10.1103/PhysRevE.73.031911View Article
  37. Angelini TE, Liang H, Wriggers W, Wong GCL: Eur. Phys. J. E. 2005, 16: 389–400. COI number [1:CAS:528:DC%2BD2MXktFOis7c%3D] 10.1140/epje/i2004-10097-9View Article
  38. Schwartz LW, Roy RV, Eley RR, Petrashy S: J. Coll. Interf. Sc.. 2001, 234: 363–374. COI number [1:CAS:528:DC%2BD3MXmt1Wmtg%3D%3D] 10.1006/jcis.2000.7312View Article
  39. Kajiya T, Nishitani E, Yamaue T, Doi M: Phys. Rev. E. 2006, 73: 011601. 10.1103/PhysRevE.73.011601View Article
  40. Shiba K, Honma T, Minamisawa T, Nishiguchi K, Noda T: EMBO Reports. 2003, 4: 148–153. COI number [1:CAS:528:DC%2BD3sXhslSqtLY%3D] 10.1038/sj.embor.embor737View Article
  41. Szabó B, Vicsek T: Phys. Rev. E. 2003, 67: 011908. 10.1103/PhysRevE.67.011908View Article
  42. Maubach G, Fritzsche W: Nano Lett.. 2004, 4: 607–611. COI number [1:CAS:528:DC%2BD2cXhvVegt7g%3D] 10.1021/nl049968+View Article
  43. Heim T, Preuss S, Gerstmayer B, Bosio A, Blossey R: J. Phys. Cond. Matt.. 2005, 17: S703-S716. COI number [1:CAS:528:DC%2BD2MXjs12ktbo%3D] 10.1088/0953-8984/17/9/025View Article
  44. Rose F, Martin P, Fujita H, Kawakatsu H: Nanotechnology. 2006, 17: 3325–3332. COI number [1:CAS:528:DC%2BD28XosFajur8%3D] 10.1088/0957-4484/17/13/041View Article
  45. A.M. Oliveira Brett, DNA-based biosensors. In: Biosensors and Modern Biospecific Analytical Techniques, Volume XLIV (Comprehensive Analytical Chemistry), ed. by L. Gorton, (Elsevier Science: Amsterdam, 2005)


© to the authors 2007