Lactobacillus assisted synthesis of titanium nanoparticles
© to the authors 2007
Received: 9 April 2007
Accepted: 17 April 2007
Published: 19 May 2007
An eco-friendlylactobacillus sp. (microbe) assisted synthesis of titanium nanoparticles is reported. The synthesis is performed at room temperature. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of Ti nanoparticles. Individual nanoparticles as well as a number of aggregates almost spherical in shape having a size of 40–60 nm are found.
KeywordsNano titanium Nanoparticles Lactobacillus sp. Eco-friendly
In recent years, materials with nano-sized dimension have attracted considerable attention of the researchers throughout the globe. In modern nano science and technology, the interaction between inorganic nanoparticles and biological structures are one of the most exciting areas of research. Also, taking into consideration the environmental, health and social aspects, there is a need to develop an eco-friendly approach for nanomaterials synthesis that should not use toxic chemicals in the synthesis protocol. This is now well known that many organisms, can produce inorganic materials either intra- or extracellularly . Bacteria, being prokaryotes have survived the test of time in enriching ions , synthesizing magnetite nanoparticles [1–3], reducing Ag into metal particles, forming nanoparticles [4, 5] and in generation of cermets . The recent discovery of the bio-synthesis [6, 7] of metal nanoparticles point towards new biotechnological methods in materials science. Nanocrystals of gold, silver and their alloys have been synthesized by the assistance of lactic acid bacterial cells . Mukherjee et al.  have successfully synthesized of gold nano-clusters using fungus. Recently, the synthesis of nanoparticles of gold , bimetallic , zinc  and even lanthanide clusters  have successfully been demonstrated using the tannins of the biomass of Medicago sativa (alfalfa). Recently seed mediated method for the synthesis of silver nanoparticles in which tannin was used to reduce silver salt in aqueous solution has been reported .
Titanium, by weight, is one of the strongest readily available metal, making it ideal for wide range of practical applications such as in automobiles, missiles, airplanes, helicopters, submarines, cathode ray tubes, batteries and even in jewelry and artificial gemstones, etc. It is 45% lighter than steel with comparable strength, and twice as strong as aluminum while being only 60% heavier. Titanium is suggested for use in desalinization plants because of its strong resistance to corrosion from sea water (particularly when coated with platinum). In medical applications titanium pins are used because of their non-reactive nature when contacting bone and flesh. Many surgical instruments, as well as body piercing are made up of titanium for this reason as well. In terms of a mechanism, TiIVbinds well to transferrin in human serum, which could deliver it to the cancer cells. This further emphasizes their future role in cancer chemotherapy and gene delivery.
Keeping in view the importance of titanium and environmental issues related to the production of nanopowders, the present work reports an eco-friendly biotechnological approach for the synthesis (lactobacillus assisted) of nano-titanium for possible applications.
Materials and methods
Results and discussion
In conclusion, the present biotechnological method is capable of producing Ti-nanoparticles. Also, it is an eco-friendly low cost approach.
- Senapati S, Mandal D, Ahmad A, Khan MI, Sastry M, Kumar R: Ind. J. Phys. 2004, 78A: 101 . COI number [1:CAS:528:DC%2BD2cXot1ylt74%3D] COI number [1:CAS:528:DC%2BD2cXot1ylt74%3D]Google Scholar
- T.J. Beveridge, R.J. Doyle (ed.), Metal Ions and Bacteria (Wiley, New York, 1989)Google Scholar
- Spring H, Schleifer KH: System Appl. Microbiol.. 1995, 18: 147.View ArticleGoogle Scholar
- Klaus T, Joerger R, Olsson E, Granqvist CG: Proc. Natl. Acad. Sci. USA. 1999, 96: 13611. COI number [1:CAS:528:DyaK1MXns1OqsL8%3D]; Bibcode number [1999PNAS...9613611K] COI number [1:CAS:528:DyaK1MXns1OqsL8%3D]; Bibcode number [1999PNAS...9613611K] 10.1073/pnas.96.24.13611View ArticleGoogle Scholar
- Klaus T, Joerger R, Olsson E, Granqvist CG: Proc. Trends Biotechnol. 2001, 19: 15 . 10.1016/S0167-7799(00)01514-6View ArticleGoogle Scholar
- Joerger R, Klaus T, Granqvist CG: Adv. Mater.. 2000, 12: 407. COI number [1:CAS:528:DC%2BD3cXkslCjsL4%3D] COI number [1:CAS:528:DC%2BD3cXkslCjsL4%3D] 10.1002/(SICI)1521-4095(200003)12:6<407::AID-ADMA407>3.0.CO;2-OView ArticleGoogle Scholar
- Tolles WM, Rath BB: Curr. Sci.. 2003, 85: 1746. COI number [1:CAS:528:DC%2BD2cXht1SgtLc%3D] COI number [1:CAS:528:DC%2BD2cXht1SgtLc%3D]Google Scholar
- Nair B, Pradeep T: Cryst. Growth Des.. 2002, 2: 293. COI number [1:CAS:528:DC%2BD38XktFaku78%3D] COI number [1:CAS:528:DC%2BD38XktFaku78%3D] 10.1021/cg0255164View ArticleGoogle Scholar
- Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Ramani R, Parischa R, Ajayakumar PV, Alam M, Sastry M, Kumar R: Angew. Chem. 2001, 40: 3585. COI number [1:CAS:528:DC%2BD3MXns1Wgsrw%3D] COI number [1:CAS:528:DC%2BD3MXns1Wgsrw%3D] 10.1002/1521-3773(20011001)40:19<3585::AID-ANIE3585>3.0.CO;2-KView ArticleGoogle Scholar
- Gardea-Torresday JL, Tiemman K, Gamez E, Dokken K, Tehuacanero S, Jose-Yacaman M: J. Nanopart. Res.. 2001, 3: 475. 10.1023/A:1012578821566View ArticleGoogle Scholar
- Ascencio JA, Mejia Y, Liu HB, Angeles C, Canizal G: Langmuir. 2003, 19: 5882 . COI number [1:CAS:528:DC%2BD3sXksFGiurY%3D] COI number [1:CAS:528:DC%2BD3sXksFGiurY%3D] 10.1021/la0340495View ArticleGoogle Scholar
- Canizal G, Retchkiman PSS, Pal U, Liu HB, Ascencio JA: Mater. Chem. Phys. 2006, 97: 321. COI number [1:CAS:528:DC%2BD28XjtFSiu7c%3D] COI number [1:CAS:528:DC%2BD28XjtFSiu7c%3D] 10.1016/j.matchemphys.2005.08.015View ArticleGoogle Scholar
- Ascencio JA, Rodr´ıguez-Monroy AC, Liu HB, Canizal G: Chem. Lett.. 2004, 33: 1056 . COI number [1:CAS:528:DC%2BD2cXmvVKls74%3D] COI number [1:CAS:528:DC%2BD2cXmvVKls74%3D] 10.1246/cl.2004.1056View ArticleGoogle Scholar
- Tian Wang Cao X W G: Mater. Lett.. 2007, 61: 130. COI number [1:CAS:528:DC%2BD28XhtF2lurnL] COI number [1:CAS:528:DC%2BD28XhtF2lurnL] 10.1016/j.matlet.2006.04.021View ArticleGoogle Scholar