The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro
© The Author(s) 2010
Received: 1 February 2010
Accepted: 5 April 2010
Published: 16 April 2010
Nanomaterials have widely been used in the field of biological and biomedicine, such as tissue imaging, diagnosis and cancer therapy. In this study, we explored the cytotoxicity and photodynamic effect of different-sized ZnO nanoparticles to target cells. Our observations demonstrated that ZnO nanoparticles exerted dose-dependent and time-dependent cytotoxicity for cancer cells like hepatocellular carcinoma SMMC-7721 cells in vitro. Meanwhile, it was observed that UV irradiation could enhance the suppression ability of ZnO nanoparticles on cancer cells proliferation, and these effects were in the size-dependent manner. Furthermore, when ZnO nanoparticles combined with daunorubicin, the related cytotoxicity of anticancer agents on cancer cells was evidently enhanced, suggesting that ZnO nanoparticles could play an important role in drug delivery. This may offer the possibility of the great potential and promising applications of the ZnO nanoparticles in clinical and biomedical areas like photodynamic cancer therapy and others.
KeywordsZnO nanoparticle SMMC-7721 Photodynamic cancer therapy (PDT) Size effect Drug delivery
With the development of nanotechnology, nanomaterials are receiving increasing interest in the relative research and industrial applications for their unique characteristics. As one of the most important application, nanomaterials are now widely studied and applied in biological and biomedical field [1–6]. Nanoscale materials, such as nanoparticles [7–9], nanorods , nanowires [11, 12], nanotubes  and nanofiber [14, 15], have been explored in many biomedical applications because of their novel properties, such as the high volume/surface ratio, surface tailorability and multifunctionality [7–10, 16–18]. Moreover, the intrinsic optical [19, 20], magnetic [21, 22] and biological properties of nanomaterials offer remarkable opportunities to study and regulate complex biological processes for biomedical applications in an unprecedented manner [23–25]. Fundamentally, life itself is a collective of processes at nanoscale within cells [15, 26].
Nanoparticles (NPs) and nanosized objects are being incorporated rapidly into clinical medicine and particularly into the field of medical oncology . As the energy donor, quantum dots have the possibility for energy transfer between quantum dot particles and cell molecules such as active oxygen and give them a potential to induce generation of reactive oxygen species and/or free radicals and to provoke apoptosis of the cells [28, 29]. The dual nature of UV-mediated cytotoxicity of quantum dots and their energy donor capacity could open a new area of quantum dot application in biology and medicine, as novel photosensitizers or at least as potentiators of the conventional photosensitizing drugs in photodynamic therapy (PDT) of cancer. Some reports have shown that some kinds of quantum dots (QDs) can act as photosensitizers and potentiators of classical photosensitizers to overcome the limitations of organic dye-based PDT [30–32].
In this study, we initially investigated the effect of different-sized ZnO nanoparticles on cytotoxicity of hepatocellular carcinoma cells (SMMC-7721). Combined with the MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide) assay, real-time cell electronic sensing (RT-CES) study and fluorescence microscope image, we explored the nanoparticles’ cytotoxicity for human cancer cells in vitro and compared the distinction of different size ZnO nanoparticles affecting the cell proliferation. As the good photocatalysis material, the photodynamic effect of these different-sized ZnO nanoparticles has also been investigated by combination with UV irradiation, which could be further utilized as a good photosensitizer with the potential application in PDT. Meanwhile, the synergetic cytotoxicity of the anticancer agent daunorubicin (DNR) with ZnO nanoparticles was explored to inhibit the cancer cell proliferation in vitro, and the real-time cell electronic sensing (RT-CES) assay also provided the dynamic process and binding behavior between cells and related agents.
Reagents and Materials
Three different-sized ZnO nanoparticles capped with aminopolysiloxane, i.e., ZP5, ZP6 and ZP7, were purchased from Jiangsu Changtai Nanometer Material Co., Ltd. The average diameters of ZP5, ZP6 and ZP7 were about 20, 60 and 100 nm, respectively, observed by transmission electron microscopy (TEM) using a JEOL JEM-2100 transmission electron microscope. Daunorubicin was purchased from Farmitalis Co. Italian. MTT was purchased from Sigma (USA), and all other agents were analytical pure.
SMMC-7721 cancer cells (purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) were maintained in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (Sigma, USA), 100 U/ml penicillin (Sigma, USA) and 100 μg/ml streptomycin (Sigma, USA) and grown at 37°C in a 5% CO2 humidified environment.
MTT Assay for the Proliferation of SMCC 7721
The effect of different size ZnO nanoparticles’ concentrations on SMMC-7721 cancer cells was observed by MTT assay. The final concentrations of ZnO nanoparticles were 1.56, 3.12, 6.25, 12.5, 25 and 50 μg/mL, respectively. Initially, 1 × 104 cells were seeded into each well containing 200 μL cell culture mediums in 96-well plate and incubated for 24 h and then added the relevant materials and incubated at 37°C with 5% CO2 for 72 h. Then, 20 μL, 5 mg/mL MTT solution was added into the well and continue cultured for about 4 h. The culture medium was discarded, and 200 μL DMSO was added following the shaking about 10 min. The UV absorption was measured at 490 nm. Controls were cultivated under the same conditions without addition of ZnO nanoparticles. The relevant experiments were repeated thrice independently. The inhibition efficiency (%) was expressed as follows: (1−[A]test/[A]control) × 100, where [A]test and [A]control represent the optical density at 490 nm for the test and control experiments, respectively.
The Effect of UV Irradiation for the Proliferation of SMCC 7721 in the Presence of ZnO Nanoparticles
The procedure of cell culture and treatment of nanoparticles was similar with the above phase of MTT assay. Upon application of UV irradiation, the UVC (λ = 254 nm) was provided by a germicidal lamp in the clean bench, and its average intensity is 0.1 mW/cm2 at the working plane. The effect of different-sized ZnO nanoparticles for SMMC 7721 cell proliferation in the presence of UV irradiation for 180 s has been explored by MTT assay. Every experiment was repeated at least three times independently.
In Vitro RT-CES Cytotoxicity Assay for SMMC-7721 Proliferation
The cell culture condition, the starting cell number, and cell culture medium volume used for the 16× sensor device were similar to that of MTT assay. Different size ZnO nanoparticles were seeded in the plate with the concentration of 2.5, 5.0, and 10.0 μg/mL, respectively. Then, the effect of UV irradiation was studied by using MTT assay. In order to study the synergistic cytotoxicity of ZnO nanoparticles and DNR, DNR was introduced into the system as the negative control with concentration about 1.0 × 10−7 mol/L. The correlative controls were also seeded in the same plate simultaneously. Once the cells were added to the sensor wells, the sensor devices were placed into the incubator, and the real-time cell index (CI) data acquisition was initiated by the RT-CES analyzer (ACEA Bioscience. Inc. USA).
Olympus IX71 Inverted Fluorescence Microscopy
The experiment was performed as described in the literature . The SMMC-7721 cells were seeded on the coverslips in six-well plates (1 × 105 cells/well) and cultured for 24 h at 37°C with 5% CO2, then daunorubicin with 1.0 × 10−5 mol/L and different size ZnO nanoparticles with 2.5 μg/mL were injected to the cells system. Meanwhile, the cells treated with the same concentration of solvent were taken as control experiments. All specimens were subsequently incubated for 1 h at 37°C with 5% CO2, and quickly washed with PBS, followed by fixation with 4% formaldehyde for 5 min. Finally, specimens were observed by inverted fluorescence microscopy (Olympus IX71, Japan).
Data were expressed as the mean ± SD (standard deviation) from at least three independent experiments. One-tailed unpaired Student’s t-test was used for significance testing, and P < 0.05 is considered significant.
Results and Discussion
Cytotoxicity of Different-Sized ZnO Nanoparticles for SMMC7721 Cancer Cells
In general, there are two mainly different actions for nanomaterials to act toxic effects on target cells: first, a chemical toxicity based on the chemical composition, such as release of (toxic) ions, particle surface catalyzed reactions or formation of reactive oxygen species; second, the stress or stimuli caused by the surface, size and/or shape of the particles . In this study, three different-sized ZnO nanoparticles could exert cytotoxic effect on SMMC-7721 cells at different concentrations. While in scale from 20 to 100 nm, there was little difference in cytotoxicity at the identical concentration of ZnO NPs. This is different from some other reports, such as the cytotoxic effect of carbon-based nanomaterials is size-dependent .
The Effect of Nano ZnO and UV Irradiation on SMMC 7721 Cell Proliferation
As the energy donors , the semiconductor nanomaterials could have a very important application in the photodynamic therapy (PDT) for energy transfer between quantum dot particles and cell molecules (such as triplet oxygen, reducing equivalents and pigments). Derfus and colleagues provoked the hypothesis that while the cytotoxicity of quantum dots, mediated by UV irradiation, is harmful for normal cell viability, it may be very useful in killing cancer cells [31, 32]. After the semiconductor, nanomaterial is excited by the UV irradiation, the semiconductor nanomaterial can release the oxygen free radical/oxyradical, and the oxyradical was considered as the main mediator of photocytotoxicity in photodynamic therapy, causing biomembrane oxidation and degradation.
Based on the effect of different-sized ZnO nanoparticles in the absence/presence of UV irradiation on SMMC 7721 cell lines, it was evident that all these ZnO nanoparticles have the similar inhibition capacity on target cancer cells, and UV irradiation could greatly enhance this inhibition effect on SMMC-7721 cells in vitro when treated with ZnO nanoparticles. These observations demonstrated that in this nano-scale level, ZnO nanoparticles could play an important role in inhibiting cancer cell proliferation, and UV irradiation can further enhance this effect. It was considered that the light excites the photosensitizing agent, resulting in formation of ROS, believed to be responsible for the cascade of cellular and molecular events in which the following result is selective tumor destruction . After UV irradiation, nano-sized ZnO particles could efficiently induce the formation of ROS and further attack the cell membrane (mainly by lipid peroxidation), proteins (such as enzyme deactivation) or even nucleic acids. And the attack of the photogenerated ROS on the cell membrane can lead to the membrane destruction, then results in the changes in the permeability on the target cell membrane, which causes the efflux of cytoplasm and the apoptosis or death of the target caner cells [28, 29, 32].
The RT-CES Dynamic Study for the Inhibition of ZnO Nanoparticles on SMMC-7721 Cancer Cells in the Presence of UV Irradiation
The real-time cell electronic sensing (RT-CES) assay could provide dynamic information to identify the interaction between target cells and reagents. The basic principle of the RT-CES system is to monitor the changes in electrode impedance induced by the interaction between testing cells and electrodes, where the presence of the cells will lead to an increase in the electrode impedance. The more cells attached to the sensor, the higher the impedance that could be monitored with RT-CES. The RT-CES array has been proven to be a valuable and reliable way for real-time monitoring of dynamic changes induced by cell-chemical interaction [38–41]. Since the relevant test is labeling free, the RT-CES assay allows real-time, automatically and continually monitoring cellular status changes during the whole process of the cell-chemical interaction. Thus, in this work we introduced the RT-CES assay to study the dynamic response of target cancer cells exposure to ZnO nanoparticles.
The Synergistic Effect of ZnO Nanoparticles for Daunorubicin Cytotoxicity
If the ZnO nanoparticles were irradiated by UV light, the generated ROS made the membrane destruction which may cause the efflux of cytoplasm and/or make more daunorubicin molecules enter into cancer cells and induce the target cell killing. So, ZnO nanoparticles could play an important role to enhance the synergistic cytotoxicity of daunorubicn in the target cancer therapy, or the ZnO nanoparticles may act as the role of drug delivery carries.
In this contribution, our observations demonstrate that different-sized ZnO nanoparticles exposed to SMMC-7721 cancer cells could exert dose-dependent cytotoxicity suppression in vitro, and this cytotoxicity of nanoparticles was time depended and dose depended, while the size-depended effect was not clear in the scope from 20 to 100 nm. UV irradiation could readily enhance the proliferation suppression ability of ZnO nanoparticles on cancer cells. More importantly, these effects were size dependent, while the smaller the nanoparticle size, the higher the cytotoxicity of cancer cell proliferation caused by ZnO nanoparticle. Meanwhile, if ZnO nanoparticles combined with daunorubicin, cytotoxicity of DNR for SMMC-7721 cancer cells was especially enhanced. These observations suggest that ZnO nanoparticles could play an important role in the PDT and have the great potential and promising applications in clinical and biomedical engineering.
This work is supported by National Natural Science Foundation of China (90713023, 20675014 and 20535010), National Basic Research Program of China (No. 2010CB732404), the Chinese Ministry of Science and Technology (2007AA022007 and 2008DFA51180) and the Natural Science Foundation of Jiangsu Province (BK2008149).
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
- Gao JH, Xu B: Nano Today. 2009, 4: 37–51. COI number [1:CAS:528:DC%2BC3cXkvFGit7s%3D] COI number [1:CAS:528:DC%2BC3cXkvFGit7s%3D] 10.1016/j.nantod.2008.10.009View ArticleGoogle Scholar
- Lewis LN: Chem. Rev.. 1993, 93: 2693–2730. COI number [1:CAS:528:DyaK3sXmsFOhs7Y%3D] COI number [1:CAS:528:DyaK3sXmsFOhs7Y%3D] 10.1021/cr00024a006View ArticleGoogle Scholar
- Yezhelyev M, Yacoub R, O’Regan R: Nanomedicine. 2009, 4: 83–103. COI number [1:CAS:528:DC%2BD1cXhsFajsbrI] COI number [1:CAS:528:DC%2BD1cXhsFajsbrI] 10.2217/174358184.108.40.206View ArticleGoogle Scholar
- Xiao Liu C: Nano Biomed. Engine. 2009,1(1):1–18.Google Scholar
- Ji JJ, Ruan J, Cui DX: Nano Biomed. Engine. 2010,2(1):100–128. COI number [1:CAS:528:DC%2BC3cXksFens7s%3D] COI number [1:CAS:528:DC%2BC3cXksFens7s%3D]Google Scholar
- Wang Z, Ruan J, Cui DX: Nanoscale Res. Lett.. 2009,4(7):593–605. COI number [1:CAS:528:DC%2BD1MXnt1Sktrw%3D]; Bibcode number [2009NRL.....4..593W] COI number [1:CAS:528:DC%2BD1MXnt1Sktrw%3D]; Bibcode number [2009NRL.....4..593W] 10.1007/s11671-009-9292-zView ArticleGoogle Scholar
- Tian FR, Prina-Mello A, Estrada G, Beyerle A, Möller W, Schulz H, Kreyling W, Stoeger T: Nano Biomed Eng.. 2009,1(1):19–38. COI number [1:CAS:528:DC%2BC3cXksFegu7Y%3D] COI number [1:CAS:528:DC%2BC3cXksFegu7Y%3D] 10.5101/nbe.v1i1.p13-26View ArticleGoogle Scholar
- Cui DX, Han YD, Li ZM, Song H, Wang K, He R, Liu B, Liu HL, Bao C, Huang P, Ruan J, Gao F, Yang H, Cho HS, Ren QS, Shi DL: Nano Biomed. Eng.. 2009,1(1):94–112. COI number [1:CAS:528:DC%2BC3cXksFensro%3D] COI number [1:CAS:528:DC%2BC3cXksFensro%3D]View ArticleGoogle Scholar
- Wang T, Hu Y, Zhang L, Jiang L, Chen Z, He NY: Nano Biomed. Eng.. 2010,2(1):46–61. COI number [1:CAS:528:DC%2BC3cXksFens74%3D] COI number [1:CAS:528:DC%2BC3cXksFens74%3D]View ArticleGoogle Scholar
- Chen SH, Ji YX, Lian Q, Wen YL, Shen HB, Jia NQ: Nano Biomed. Eng.. 2010,2(1):19–31. COI number [1:CAS:528:DC%2BC3cXksFensrY%3D] COI number [1:CAS:528:DC%2BC3cXksFensrY%3D]Google Scholar
- Bechara SL, Judson A, Popat KC: Biomaterials. 2010,31(13):3492–3501. COI number [1:CAS:528:DC%2BC3cXisFSlsLk%3D] COI number [1:CAS:528:DC%2BC3cXisFSlsLk%3D] 10.1016/j.biomaterials.2010.01.084View ArticleGoogle Scholar
- Bellamkonda R, John T, Mathew B, DeCoster M, Hegab H, Davis DJ: Micromech. Microeng.. 2010,20(2):1–6. COI number [1:CAS:528:DC%2BC3cXit1Wlsr0%3D] COI number [1:CAS:528:DC%2BC3cXit1Wlsr0%3D] 10.1088/0960-1317/20/2/025012View ArticleGoogle Scholar
- Chen DF, Wu XB, Wang JX, Han BS, Zhu P, Peng CH: Nano Biomed. Eng.. 2010,1(1):119–129.Google Scholar
- He CL, Zhang L, Wang HS, Zhang F, Mo XM: Nano Biomed. Eng.. 2009,2(1):91–99.Google Scholar
- Li YQ, Li ZY, Zhou XP, Yang P: Nano Biomed. Eng.. 2010,2(1):32–45. COI number [1:CAS:528:DC%2BC3cXksFensrc%3D] COI number [1:CAS:528:DC%2BC3cXksFensrc%3D] 10.5101/nbe.v2i1.p24-30View ArticleGoogle Scholar
- Hong S, Leroueil PR, Janus EK, Peters JL, Kober MM, Islam MT, Orr BG, Baker JR, Holl MB: Bioconjugate Chem.. 2006, 17: 728–734. COI number [1:CAS:528:DC%2BD28XktVSksr0%3D] COI number [1:CAS:528:DC%2BD28XktVSksr0%3D] 10.1021/bc060077yView ArticleGoogle Scholar
- Mecke A, Lee DK, Ramamoorthy A, Orr BG, Holl MB: Biophys. J.. 2005, 89: 4043–4050. COI number [1:CAS:528:DC%2BD2MXhtlWms77K] COI number [1:CAS:528:DC%2BD2MXhtlWms77K] 10.1529/biophysj.105.062596View ArticleGoogle Scholar
- Park H, Lee S, Chen LX, Lee EK, Shin SY, Lee YH, Son SW, Oh CH, Song JM, Kang SH, Choo J: Phys. Chem. Chem. Phys.. 2009, 11: 7444–7449. COI number [1:CAS:528:DC%2BD1MXhtVSmtrrJ] COI number [1:CAS:528:DC%2BD1MXhtVSmtrrJ] 10.1039/b904592aView ArticleGoogle Scholar
- Fisher BR, Eisler HJ, Stott NE, Bawendi MG: J. Phys. Chem. B.. 2004, 108: 143–148. COI number [1:CAS:528:DC%2BD3sXps1SmsbY%3D] COI number [1:CAS:528:DC%2BD3sXps1SmsbY%3D] 10.1021/jp035756+View ArticleGoogle Scholar
- Everts M, Saini V, Leddon JL, Kok RJ, Stoff-Khalili M, Preuss MA, Millican CL, Perkins G, Brown JM, Bagaria H, Nikles DE, Johnson DT, Zharov VP, Curiel DT: Nano. Lett.. 2006, 6: 587–591. COI number [1:CAS:528:DC%2BD28Xit1OrsLs%3D]; Bibcode number [2006NanoL...6..587E] COI number [1:CAS:528:DC%2BD28Xit1OrsLs%3D]; Bibcode number [2006NanoL...6..587E] 10.1021/nl0500555View ArticleGoogle Scholar
- Lin XM, Sorensen CM, Klabunde KJ, Hadjipanayis GC: Langmuir. 1998, 14: 7140–7146. COI number [1:CAS:528:DyaK1cXntlansr8%3D] COI number [1:CAS:528:DyaK1cXntlansr8%3D] 10.1021/la980509wView ArticleGoogle Scholar
- Sonvico F, Mornet S, Vasseur S, Dubernet C, Jaillard D, Degrouard J, Hoebeke J, Duguet E, Colombo P, Couvreur P: Bioconjugate Chem.. 2005, 16: 1181–1188. COI number [1:CAS:528:DC%2BD2MXpsleqsrs%3D] COI number [1:CAS:528:DC%2BD2MXpsleqsrs%3D] 10.1021/bc050050zView ArticleGoogle Scholar
- Allen TM, Cullis PR: Science. 2004, 303: 1818–1822. COI number [1:CAS:528:DC%2BD2cXitFehu70%3D]; Bibcode number [2004Sci...303.1818A] COI number [1:CAS:528:DC%2BD2cXitFehu70%3D]; Bibcode number [2004Sci...303.1818A] 10.1126/science.1095833View ArticleGoogle Scholar
- Wang G, Huang T, Murray RW, Menard L, Nuzzo RG: J. Am. Chem. Soc.. 2005, 127: 812–813. COI number [1:CAS:528:DC%2BD2MXkvVOmsQ%3D%3D] COI number [1:CAS:528:DC%2BD2MXkvVOmsQ%3D%3D] 10.1021/ja0452471View ArticleGoogle Scholar
- Tiefenauer LX, Kuhne G, Andres RY: Bioconjugate Chem.. 1993,4(5):347–352. COI number [1:CAS:528:DyaK3sXlslKktrY%3D] COI number [1:CAS:528:DyaK3sXlslKktrY%3D] 10.1021/bc00023a007View ArticleGoogle Scholar
- Mann S: Angew. Chem. Int. Ed.. 2008, 47: 5306–5320. COI number [1:CAS:528:DC%2BD1cXovF2lu7o%3D] COI number [1:CAS:528:DC%2BD1cXovF2lu7o%3D] 10.1002/anie.200705538View ArticleGoogle Scholar
- Bao CC, Yang H, Sheng P, Song H, Ding XH, Liu B, Lu YC, Hu GH, Cui DX: Nano Biomed. Eng.. 2010,1(1):74–87.Google Scholar
- Claap AR, Medintz IL, Mauro JM, Fisher BR, Bawendi MG, Mattoussi H: J. Am. Chem. Soc.. 2004, 126: 301–310. COI number [1:CAS:528:DC%2BD3sXpslWntr8%3D] COI number [1:CAS:528:DC%2BD3sXpslWntr8%3D] 10.1021/ja037088bView ArticleGoogle Scholar
- Bakalova R, Ohba H, Zhelev Z, Nagase T, Jose R, Ishikawa M, Baba Y: Nano Lett.. 2004,4(9):1567–1573. COI number [1:CAS:528:DC%2BD2cXmtVKlu7c%3D]; Bibcode number [2004NanoL...4.1567B] COI number [1:CAS:528:DC%2BD2cXmtVKlu7c%3D]; Bibcode number [2004NanoL...4.1567B] 10.1021/nl049627wView ArticleGoogle Scholar
- Delehanty JB, Boeneman K, Bradburne CE, Robertson K, Medintz IL: Expert. Opin. Drug Deliv.. 2009,6(10):1091–1112. COI number [1:CAS:528:DC%2BD1MXhtFGhur3P] COI number [1:CAS:528:DC%2BD1MXhtFGhur3P] 10.1517/17425240903167934View ArticleGoogle Scholar
- Derfus AM, Chan WC, Bhatia SN: Nano Lett.. 2004,4(1):11–18. COI number [1:CAS:528:DC%2BD3sXps1SmtLo%3D]; Bibcode number [2004NanoL...4...11D] COI number [1:CAS:528:DC%2BD3sXps1SmtLo%3D]; Bibcode number [2004NanoL...4...11D] 10.1021/nl0347334View ArticleGoogle Scholar
- Macdonald IJ, Dougherty TJ: J. Porphyr. Phthalocyanines. 2001, 5: 105–129. COI number [1:CAS:528:DC%2BD3MXht1SgtLY%3D] COI number [1:CAS:528:DC%2BD3MXht1SgtLY%3D] 10.1002/jpp.328View ArticleGoogle Scholar
- Nifli AP, Theodoropoulos PA, Munier S, Castagnino C, Roussakis E, Katerinopoulos HE, Vercauteren J, Castanas EJ: J. Agric. Food Chem.. 2007, 55: 2873–2878. COI number [1:CAS:528:DC%2BD2sXjsVOrsLo%3D] COI number [1:CAS:528:DC%2BD2sXjsVOrsLo%3D] 10.1021/jf0632637View ArticleGoogle Scholar
- Guo DD, Wu CH, Li JY, Guo AR, Li QN, Jiang H, Chen BA, Wang XM: Nanoscale Res. Lett.. 2009, 4: 1395–1402. COI number [1:CAS:528:DC%2BD1MXhsFWqsbnK]; Bibcode number [2009NRL.....4.1395G] COI number [1:CAS:528:DC%2BD1MXhsFWqsbnK]; Bibcode number [2009NRL.....4.1395G] 10.1007/s11671-009-9411-xView ArticleGoogle Scholar
- Brunner TJ, Wick P, Manser P, Spohn P, Grass RN, Limbach LK, Bruinink A, Stark WJ: Environ. Sci. Technol.. 2006, 40: 4374–4381. COI number [1:CAS:528:DC%2BD28Xit1Wms7g%3D] COI number [1:CAS:528:DC%2BD28Xit1Wms7g%3D] 10.1021/es052069iView ArticleGoogle Scholar
- Magrez S, Kasas S, Salicio V, Pasquier N, Seo JW, Celio M, Catsicas S, Schwaller B, Forro l: Nano Lett.. 2006, 6: 1121–1125. COI number [1:CAS:528:DC%2BD28Xksl2ktrw%3D]; Bibcode number [2006NanoL...6.1121M] COI number [1:CAS:528:DC%2BD28Xksl2ktrw%3D]; Bibcode number [2006NanoL...6.1121M] 10.1021/nl060162eView ArticleGoogle Scholar
- Dougherty TJ, Gomer CJ, Henderson BW, Jori G, Kessel D, Korbelik M, Moan J, Peng Q: J Natl. Cancer Inst.. 1998, 90: 889–905. COI number [1:CAS:528:DyaK1cXktFOqsLY%3D] COI number [1:CAS:528:DyaK1cXktFOqsLY%3D] 10.1093/jnci/90.12.889View ArticleGoogle Scholar
- Xing JZ, Zhu LJ, Gabos S, Xie L: Toxicol. In Vitro. 2006, 20: 995–1004. COI number [1:CAS:528:DC%2BD28Xmt1Oru70%3D] COI number [1:CAS:528:DC%2BD28Xmt1Oru70%3D] 10.1016/j.tiv.2005.12.008View ArticleGoogle Scholar
- Xing JZ, Zhu L, Jackson JA, Gabos S, Sun XJ, Wang XB, Xu X: Chem. Res. Toxicol.. 2005, 18: 154–161. COI number [1:CAS:528:DC%2BD2MXps1Ogtw%3D%3D] COI number [1:CAS:528:DC%2BD2MXps1Ogtw%3D%3D] 10.1021/tx049721sView ArticleGoogle Scholar
- Zhu J, Wang XB, Xu X, Abassi YA: J. Immunol. Methods. 2006, 309: 25–33. COI number [1:CAS:528:DC%2BD28XhtlGmsLY%3D] COI number [1:CAS:528:DC%2BD28XhtlGmsLY%3D] 10.1016/j.jim.2005.10.018View ArticleGoogle Scholar
- Mossman T: Immunol. Methods. 1983, 65: 55–63. 10.1016/0022-1759(83)90303-4View ArticleGoogle Scholar
- Matsumura Y, Gotoh M, Muro K, Yamada Y, Shirao K, Shimada Y, Okuwa M, Matsumoto S, Miyata Y, Ohkura H, Chin K, Baba S, Yamao T, Kannami A, Takamatsu Y, It K, Takahashi K: Ann. Oncol.. 2004, 15: 517–525. COI number [1:STN:280:DC%2BD2c7gvVersQ%3D%3D] COI number [1:STN:280:DC%2BD2c7gvVersQ%3D%3D] 10.1093/annonc/mdh092View ArticleGoogle Scholar