Novel docetaxel-loaded nanoparticles based on poly(lactide-co-caprolactone) and poly(lactide-co-glycolide-co-caprolactone) for prostate cancer treatment: formulation, characterization, and cytotoxicity studies
- Vanna Sanna†1Email author,
- Anna Maria Roggio†1,
- Anna Maria Posadino2,
- Annalisa Cossu2,
- Salvatore Marceddu3,
- Alberto Mariani4,
- Valeria Alzari4,
- Sergio Uzzau1, 2,
- Gianfranco Pintus2 and
- Mario Sechi5
© Sanna et al; licensee Springer. 2011
Received: 16 December 2010
Accepted: 28 March 2011
Published: 28 March 2011
Docetaxel (Dtx) chemotherapy is the optional treatment in patients with hormone-refractory metastatic prostate cancer, and Dtx-loaded polymeric nanoparticles (NPs) have the potential to induce durable clinical responses. However, alternative formulations are needed to overcome the serious side effects, also due to the adjuvant used, and to improve the clinical efficacy of the drug.
In the present study, two novel biodegradable block-copolymers, poly(lactide-co-caprolactone) (PLA-PCL) and poly(lactide-co-caprolactone-co-glycolide) (PLGA-PCL), were explored for the formulation of Dtx-loaded NPs and compared with PLA- and PLGA-NPs. The nanosystems were prepared by an original nanoprecipitation method, using Pluronic F-127 as surfactant agent, and were characterized in terms of morphology, size distribution, encapsulation efficiency, crystalline structure, and in vitro release. To evaluate the potential anticancer efficacy of a nanoparticulate system, in vitro cytotoxicity studies on human prostate cancer cell line (PC3) were carried out. NPs were found to be of spherical shape with an average diameter in the range of 100 to 200 nm and a unimodal particle size distribution. Dtx was incorporated into the PLGA-PCL NPs with higher (p < 0.05) encapsulation efficiency than that of other polymers. Differential scanning calorimetry suggested that Dtx was molecularly dispersed in the polymeric matrices. In vitro drug release study showed that release profiles of Dtx varied on the bases of characteristics of polymers used for formulation. PLA-PCL and PLGA-PCL drug loaded NPs shared an overlapping release profiles, and are able to release about 90% of drug within 6 h, when compared with PLA- and PLGA-NPs. Moreover, cytotoxicity studies demonstrated advantages of the Dtx-loaded PLGA-PCL NPs over pure Dtx in both time- and concentration-dependent manner. In particular, an increase of 20% of PC3 growth inhibition was determined by PLGA-PCL NPs with respect to free drug after 72 h incubation and at all tested Dtx concentration. In summary, PLGA-PCL copolymer may be considered as an attractive and promising polymeric material for the formulation of Dtx NPs as delivery system for prostate cancer treatment, and can also be pursued as a validated system in a more large context.
Prostate cancer (PCa) is the most common male malignancy and the second leading cause of cancer death in Western industrialized countries . The PCa progression does not only mean distant metastases but also development of hormone independence of its cells .
Docetaxel (Dtx)-based regimen is recommended as optional treatment in patients with hormone-refractory metastatic PCa, showing effective on locally advanced PCa and increasing overall survival . Despite the recently reported promising outcome of Dtx, this drug is associated with systemic toxicity that limits the dose and duration of therapy, particularly in elderly patients. Adverse effects of Dtx treatment include hypersensitivity reactions, bone marrow suppression, cutaneous reactions, fluid retention, peripheral neuropathy, alopecia, cardiac disorders, and tiredness . Moreover, to facilitate its clinical use, the Dtx poor water solubility requires a specific solvent system, such as ethanol/Tween 80 solution, which causes hypersensitivity reaction, reduced uptake by tumor tissue and increased exposure to other body compartments .
Therefore, alternative Dtx formulations are needed and nanoparticles (NPs)-based drug delivery systems are of special interest in addressing the major side effects of anticancer drug.
Recently, a number of polymers have been investigated for formulating biodegradable Dtx-loaded NPs for cancer chemotherapy. The most widely used class of biocompatible and biodegradable polymers, approved by Food and Drug Administration (FDA), is that of aliphatic polyesters, including poly(lactic acid) (PLA), poly(glycolic acid) (PLGA), and their copolymers .
However, these polymers are characterized by a high hydrophobicity and slow degradation rate that can represent in some cases a disadvantage for their use as drug carriers .
In the family of polyesters, polycaprolactone (PCL) shows an excellent biocompatibility and rapid degradability, good miscibility with a variety of polymers and great permeability, which make it a suitable candidate for drug carrier .
The methods for the preparation of NPs from the preformed polymers include nanoprecipitation, emulsification/solvent evaporation, emulsification/solvent diffusion, and salting out . As for the nanoprecipitation, the particle formation is based on precipitation and subsequent solidification of the polymer at the interface between a solvent and a non-solvent . Usually, surfactants or stabilizers are included in the process to modify the size and the surface properties, and to ensure the stability of the NP dispersion . The most popular stabilizer for the production of PLGA-based NPs is poly(vinyl alcohol) (PVA). However, it has been found that residual PVA, which is very difficult to remove from the particles' surface, causes relatively lower cellular uptake, thus resulting not satisfactorily biocompatible for the human body . Therefore, other surfactants could be considered as valuable alternatives to PVA. Poloxamer, a block copolymer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), commercially known as Pluronic, is another promising surface active agent approved by FDA for clinical use . Additionally, several evidence demonstrated that poloxamers interact with multidrug-resistant tumors, resulting in a drastic sensitization of these tumors with respect to various anticancer agents [13, 14].
The present work is aimed at investigating the influence of two previously unexplored biodegradable block-copolymers, such as poly(D,L-lactide-co-caprolactone) (PLA-PCL) and poly(L-lactide-co-caprolactone-co-glycolide) (PLGA-PCL), on the physicochemical and pharmaceutical properties of novel Dtx-loaded NPs, using PLA- and PLGA-NPs as comparison. The designed nanosystems were obtained by a modified nanoprecipitation technique, using Pluronic F-127 instead of PVA as stabilizing agent. The NPs were characterized in terms of morphology, encapsulation efficiency and crystalline structure, and in vitro release kinetics. Moreover, the antiproliferative efficacy of the most promising nanoparticulate prototype was assessed by in vitro cytotoxicity assay toward a human prostate cancer cell line (PC3).
Materials and methods
Poly(D,L-lactide) (PLA) (inherent viscosity 0.55 to 0.75 dl/g in chloroform), poly(D,L-lactide-co-glycolide) (PLGA) (lactide:glycolide 50:50 molar ratio, inherent viscosity 0.55 to 0.75 dl/g in hexafluoroisopropanol) with esters end groups were purchased from Lactel Absorbable Polymers (Pelham, AL, USA). Poly(L-lactide-co-caprolactone-co-glycolide) (PLGA-PCL) (L-lactide:glycolide:caprolactone 70:10:20 molar ratio, inherent viscosity 1.30 dl/g, in chloroform) was obtained from Sigma-Aldrich (Steinheim, Germany). Poly(L-lactide-co-caprolactone) (PLA-PCL) (lactide:caprolactone 70:30 molar ratio) was kindly provided from Purac Biomaterials (Gorinchen, Netherlands). Pluronic® F-127 was obtained from Sigma-Aldrich (Steinheim, Germany). Docetaxel (purity 99%) was supplied by ChemieTek (Indianapolis, IN, USA). All other chemicals were analytical grade and were used without further purification.
Formulation of nanoparticles
Drug-loaded NPs were prepared by a novel nanoprecipitation method. Briefly, about 100 mg of polymer and drug (1%, w/w) were dissolved in 6 ml of acetonitrile. The organic solution was then added dropwise under stirring to an aqueous solution of Pluronic F-127 (1% w/v, 30 ml). The milky colloidal suspension was evaporated at room temperature to remove the organic solvent. The suspension was centrifuged at 13,000 rpm for 10 min to separate the NPs from the unencapsulated drug and residual Pluronic. The pellet was washed thrice with ultrapure water. Drug-free NPs were produced in a similar manner. The obtained NP suspension was used immediately for assay or lyophilized for storage at -50°C.
Characterization of NPs
Particle size and size distribution
Mean diameter and size distribution of the NPs were measured by photon correlation spectroscopy with Coulter N4 plus particle size analyzer (Beckman Coulter, Fullerton, CA, USA). The samples were diluted with deionized water and sonicated for 5 min before measurement. Each analysis was carried out in triplicate.
The morphological examination of NPs was performed by scanning electron microscopy (SEM) (model DSM 962; Carl Zeiss Inc., Oberkochen, German). A drop of NPs suspension was placed on a glass cover slide and dried under vacuum for 12 h. After that, the slides were mounted on aluminum stub and the samples were then analyzed at 20 kV acceleration voltage after gold sputtering, under an argon atmosphere.
Encapsulation efficiency and yield of production
To determine the drug loading, an aliquot of NPs (1.0 mg) was dissolved in 0.25 ml of acetonitrile/water (50/50, v/v) solution. Samples were centrifuged and the drug content in the supernatant was analyzed by HPLC using the method previously reported . The equipment consisted of an HPLC system including an autosampler and a diode array detector (HP 1200, Agilent Technologies, Santa Clara, CA, USA). The chromatographic separation was performed at room temperature using a Jupiter C18 column (250 mm × 2.0 mm, 5 μm pore size, Phenomenex) with a flow rate of 0.2 ml/min. Twenty microlitres of samples or calibration standards were injected into the column and eluted with a solution of acetonitrile/water solution (50/50, v/v). Detection was carried out by monitoring the absorbance signals at 227 nm. The elution period was 25 min and the retention time of Dtx was about 9 min. HPLC was calibrated with standard solutions of 5 to 50 μg/ml of Dtx (correlation coefficient of R 2 = 0.9999).
The drug encapsulation efficiency (EE%) was calculated as the ratio between the amount of Dtx encapsulated in NPs and the weight of drug used for NP preparation.
The lyophilized NPs from each formulation were weighed and the respective percentage yield of production was calculated as the ratio between the amount of NPs weight obtained and the total weight of solid materials used for the preparations.
Differential scanning calorimetry (DSC)
DSC scans of empty and drug-loaded NPs were performed on a DSC Q100 V 9.0 calorimeter (TA Instrument, New Castle, USA). Indium was used to calibrate the instrument. The thermograms of samples were obtained at a scanning rate of 10°C/min in 30 to 200°C temperature range and performed under an Ar purge (50 ml/min). The thermal measurements were carried out on pure Dtx, on freeze-dried empty and drug-loaded NPs.
In vitro drug release
For the in vitro release studies, about 10 mg of Dtx-NPs were suspended in 2.0 ml of release medium (phosphate buffer solution of pH 7.4 containing 0.1% w/v Tween 80). The tubes were then incubated at 37°C and shaken horizontally at 200 rpm (Thermomixer HLC BioTech, Bovenden, Germany). At predetermined time intervals, the tubes were centrifuged at 13,000 rpm for 10 min and 1.0 ml aliquots of medium were taken from supernatant and replaced with fresh solution. The collected samples were extracted with 0.2 ml dichloromethane, evaporated by nitrogen stream, and reconstituted in 100 μl mobile phase.
The concentration of Dtx released from the NPs was determined by HPLC assay as described above and corrected for the dilution due to the addition of fresh medium.
In vitro cytotoxicity assay
With respect to particle size, encapsulation efficiency, and yield of production results, the PLGA-PCL Dtx-loaded NPs were selected for the in vitro cytotoxicity studies. A batch of empty PLGA-PCL NPs was used as comparison.
Cancer cell viability of the drug-loaded NPs was evaluated by MTT assay. Hundred microliters of PC3 cells were seeded in 96-well plates (Costar, IL, USA) at the density of 5,000 viable cells/well and allowed to adhere for 24 h.
where Abssample is the absorbance of the cells incubated with the NP suspension, and Abscontrol is the absorbance of the cells incubated with the culture medium only (positive control). All experiments were repeated thrice.
The data are expressed as mean ± standard deviation (S.D.). The significance of differences was assessed by one-way analysis of variance (ANOVA) (Origin®, version 7.0 SR0, OriginLab Corporation, Northampton, MA, USA) and considered significant when p < 0.05.
Results and discussion
Characterization of NPs
Size, drug entrapment efficiency, and yields of production
The nanoprecipitation method described here appeared to be a suitable technique to formulate Dtx-loaded NPs based on PLA and PLGA and their poly-caprolactone copolymers. The preparation was carried out by using a polymer concentration in organic phase of 3.3 mg/ml and a solvent:Pluronic® F-127 solution ratio of 1:5. Acetonitrile was selected as organic phase for the capability to solubilize the polymers and drug and its good water miscibility, that allows a fine polymer dispersion into water.
Moreover, although the most commonly used PVA emulsifier can make uniform particles with small size, it has been recently demonstrated that it can form an interconnected network with the PLGA polymer at the interface that would compromise the good redispersibility of NPs . Additionally, the residual PVA associated with the surface of NPs would influence their physical properties and cellular uptake. Therefore, due to the above reasons, PVA was conveniently replaced with Pluronic in the current study.
The effect of the different type of polymers on characteristic of NPs.
146.3 ± 23.5
0.198 ± 0.03
37.8 ± 3.0
51.9 ± 1.4
106.7 ± 13.5
0.108 ± 0.04
35.2 ± 2.6
68.2 ± 2.7
194.8 ± 31.2
0.308 ± 0.08
19.7 ± 1.4
36.8 ± 1.6
114.8 ± 15.4
0.258 ± 0.07
47.2 ± 3.8
83.9 ± 3.3
As far as the drug entrapment efficiency (EE) is concerned, from drug content analysis no significant differences were found for the batches based on PLA and PLGA polymers (38 and 35%, respectively). On the other hand, relevant differences are obtained for NPs formulated with both copolymers containing PCL. The PLGA-PCL NPs exhibited significantly (p < 0.05) higher drug encapsulation efficiency (47%) compared with other formulations.
This finding can be ascribed to the presence of three polymers, PLA, PLGA, and PCL, which are able to generate a polymer network characterized by a greatly irregular and disordered crystalline structure, which may consequently promote the accommodation of the drug molecules.
Conversely, the lower percentages of encapsulation efficiency and NP yield obtained with PLA-PCL copolymer might be explained with a partial and fast precipitation of polymer that occurs after addition of organic phase to surfactant solution. Yields of production ranged between 52 and 84%, with the highest values for NPs based on PLGA-PCL copolymers.
Furthermore, the size and particle size distribution of unloaded NPs were similar to the corresponding loaded batches, thus indicating that the presence of the drug did not influence these characteristics (data not shown).
The partial fusion detected in some NP samples takes place during the analysis, where many NPs congregate together. Due to presence of the drug, no difference in the morphological properties of NPs drug was observed due to the presence of the drug.
Differential scanning calorimetry (DSC)
In all cases, the DSC scans revealed that Dtx melting peak totally disappeared in the calorimetric curve of loaded NPs, thus evidencing the absence of crystalline drug in the samples. These results suggested that the nanoencapsulation inhibited the crystallization of Dtx during NPs formation. Thus, it can be assumed that Dtx was present in the NPs either in an amorphous or disordered crystalline phase or in the solid solution state .
Meanwhile, the loaded systems exhibited the same endothermic events (at 58°C for PLA, 52°C for PLGA, 54°C for PLA-PCL, and 50 and 113°C for PLGA-PCL) of the corresponding unloaded NPs, referring to melting temperature (Tm) of polymers (Figure 3a to 3d), suggesting that the nanoencapsulation procedure did not affect the polymer structure.
In vitro drug release
In order to investigate the kinetic modeling of drug release from NPs, the dissolution profiles were fitted to zero order (Q = k 0·t); first order (ln (100 - Q) = ln Q 0 - k 1·t), Higuchi (Q = k H·t 1/2), and Korsmeyer-Peppas models .
Correlation coefficients (r 2) and release exponent (n) of kinetic data analysis of Dtx release from different polymeric NPs.
Zero order r 2
First order r 2
Higuchi r 2
In vitro cytotoxicity assay
The in vitro cytotoxicity of Dtx both as free drug and loaded into PLGA-PCL NPs, at the same drug equivalent concentration of 0.05, 0.5, and 5.0 μg/ml, was evaluated by the MTT assay using PC3 as model prostate cancer cell line. Untreated PC3 cells as well as cells treated with empty PLGA-PCL NPs, with the same polymer content of that of the drug-loaded nanosystems, were used as comparison.
Since no significant influence on cell viability was detected, drug-free NPs appeared nearly non-toxic to PC3 cells, even at a high NP concentration, thus confirming the good biocompatibility of PLGA-PCL copolymer .
On the whole, the results clearly demonstrated that Dtx formulated in PLGA-PCL NPs resulted more effective against cancer cells than free Dtx. Both the dose exposure and the incubation time played a major role in the cell toxicity of Dtx.
In particular, after 24 h incubation, the PC3 cell viability was decreased to about 65, 55, and 40% for PLGA-PCL NPs at 0.05, 0.5, and 5.0 μg/ml drug concentration, respectively, corresponding to an increase in cytotoxicity of 25% (p < 0.05) compared with that of free Dtx.
After 48 h incubation, the cytotoxicity was increased about 55, 60, and 70% for the PLGA-PCL-Dtx NPs and 25, 35, and 45% for free drug, respectively.
The more marked inhibition of cell growth was obtained for longer incubation period (72 h); at all tested Dtx concentration, PLGA-PCL NPs determined a significant (p < 0.05) increased of PC3 growth inhibition (about 20%) with respect to free drug. With an approximately 85% cell viability reduction, the strongest cytotoxic effect was achieved with NPs at 5.0 μg/ml Dtx concentration.
This finding is related with the mechanism of action of Dtx, which requires cell division to operate. In fact, for longer incubation periods, a larger number of cells enter G2 and M cell cycle phases, where Dtx selectively works .
According to literature data, the higher cytotoxicity of the drug formulated into NPs can be attributed to the combination of different but not exclusive mechanisms. In fact, the NPs were adsorbed on the cell surface leading to an increase in drug concentration near the cell membrane, thus generating a concentration gradient that promotes the drug influx into the cell . Besides, while part of free Dtx molecules, transported into the cytoplasm by a passive diffusion, were transported out by P-glycoprotein (P-gp) pumps, NPs are taken up by cells through an endocytosis pathway, thus resulting in a higher cellular uptake of the entrapped drug , thereby enabling them to escape from the effect of P-gp pumps. Moreover, intracellular delivery of Dtx NPs allows a drug accumulation near the site of action .
In this work, poly(lactide-co-caprolactone) and poly(lactide-co-caprolactone-co-glycolide) copolymers were successfully employed for the first time for the nanoencapsulation of Dtx, by using a modified nanoprecipitation method. Among the investigated copolymers, PLGA-PCL resulted more effective than PLA-PCL-NPs to achieve NPs with small size, uniform distribution, and higher encapsulation efficiency. The in vitro studies proved an increased antiproliferative efficacy of PLGA-PCL-Dtx NPs compared with free Dtx, in a dose- and time-dependent manner, against PC3 cells.
In conclusion, the PLGA-PCL copolymer can be considered as alternative and promising biocompatible polymer to be used on development of NP-based drug delivery system for cancer chemotherapy.
The research activities presented were done within the frame of 'Progetto Cluster - Sviluppo ed Utilizzo di Nanodevices', funded by Sardinian Technology Park - Porto Conte Ricerche, Italy. A grant from Banco di Sardegna Foundation to GP also partially supported this work. The authors thank the Company Purac Biomaterials (Gorinchen, Netherlands) for providing PLA-PCL polymer.
- Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ: Cancer statistics, 2009. CA Cancer J Clin 2009, 59: 225–249. 10.3322/caac.20006View Article
- Petrylak DP, Tangen CM, Hussain MH, Lara PN Jr, Jones JA, Taplin ME, Burch PA, Berry D, Moinpour C, Kohli M, Benson MC, Small EJ, Raghavan D, Crawford ED: Docetaxel and estramustine compared with mitoxantrone and prednisone for advanced refractory prostate cancer. N Engl J Med 2004, 351: 1513–1520. 10.1056/NEJMoa041318View Article
- Tannock IF, de Wit R, Berry WR, Horti J, Pluzanska A, Chi KN, Oudard S, Théodore C, James ND, Turesson I, Rosenthal MA, Eisenberger MA, TAX 327 Investigators: Docetaxel plus prednisone or mitoxantrone plus prednisone for advanced prostate cancer. N Engl J Med 2004, 351: 1502–1152. 10.1056/NEJMoa040720View Article
- Petrylak DP: The treatment of hormone-refractory prostate cancer: docetaxel and beyond. Rev Urol 2006, 8: S48-S55.
- Lee E, Kim H, Lee IH, Jon S: In vivo antitumor effects of chitosan-conjugated docetaxel after oral administration. J Control Release 2009, 140: 79–85. 10.1016/j.jconrel.2009.08.014View Article
- Musumeci T, Ventura C, Giannone I, Ruozi B, Montenegro L, Pignatello R, Puglisi G: PLA/PLGA nanoparticles for sustained release of docetaxel. Int J Pharm 2006, 325: 172–179. 10.1016/j.ijpharm.2006.06.023View Article
- Zhang Z, Feng SS: Nanoparticles of poly(lactide)/vitamin E TPGS copolymer for cancer chemotherapy: synthesis, formulation, characterization and in vitro drug release. Biomaterials 2006, 27: 262–270. 10.1016/j.biomaterials.2005.05.104View Article
- Zhang H, Cui W, Bei J, Wang S: Preparation of poly(lactide-co-glycolide-co-caprolactone) nanoparticles and their degradation behaviour in aqueous solution. Polym Degrad Stab 2006, 91: 1929–1936. 10.1016/j.polymdegradstab.2006.03.004View Article
- Hans ML, Lowman AM: Biodegradable nanoparticles for drug delivery and targeting. Curr Opin Solid State Mater Sci 2002, 6: 319–327. 10.1016/S1359-0286(02)00117-1View Article
- Pinto Reis C, Neufeld RJ, Ribeiro AJ, Veiga F: Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomedicine 2006, 2: 8–21.View Article
- Sahoo SK, Panyam J, Prabha S, Labhasetwar V: Residual polyvinyl alcohol associated with poly(D,L-lactide-co-glycolide) nanoparticles affects their physical properties and cellular uptake. J Control Release 2002, 82: 105–114. 10.1016/S0168-3659(02)00127-XView Article
- Khattak SF, Bhatia SR, Roberts SC: Pluronic F127 as a cell encapsulation material: utilization of membrane-stabilizing agents. Tissue Eng 2005, 11: 974–983. 10.1089/ten.2005.11.974View Article
- Moghimi SM, Hunter AC: Poloxamers and poloxamines in nanoparticle engineering and experimental medicine. Trends Biotechnol 2000, 18: 412–420. 10.1016/S0167-7799(00)01485-2View Article
- Yan F, Zhang C, Zheng Y, Mei L, Tang L, Song C, Sun H, Huang L: The effect of poloxamer 188 on nanoparticle morphology, size, cancer cell uptake, and cytotoxicity. Nanomedicine 2010, 6: 170–178.View Article
- Immordino ML, Brusa P, Arpicco S, Stella B, Dosio F, Cattel L: Preparation, characterization, cytotoxicity and pharmacokinetics of liposomes containing docetaxel. J Control Release 2003, 91: 417–429. 10.1016/S0168-3659(03)00271-2View Article
- Fonseca C, Simoes S, Gaspar R: Paclitaxel-loaded PLGA nanoparticles: preparation, physicochemical characterization and in vitro anti-tumoral activity. J Control Release 2002, 83: 273–286. 10.1016/S0168-3659(02)00212-2View Article
- Cho K, Wang X, Nie S, Chen Z, Shin DM: Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res 2008, 14: 1310–1316. 10.1158/1078-0432.CCR-07-1441View Article
- Mehta AK, Yadav KS, Sawant KK: Nimodipine loaded PLGA nanoparticles: formulation optimization using factorial design, characterization and in vitro evaluation. Curr Drug Deliv 2007, 4: 185–193. 10.2174/156720107781023929View Article
- Korsmeyer RW, Gurney R, Doelker E, Buri P, Peppas NA: Mechanism of solute release from porous hydrophilic polymer. J Pharm Sci 1983, 15: 25–35.
- Jeong SI, Kim BS, Kang SW, Kwon JH, Lee YM, Kim SH, Kim YH: In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-epsilon-caprolactone) scaffolds. Biomaterials 2004, 25: 5939–5946. 10.1016/j.biomaterials.2004.01.057View Article
- Luo Y, Ling Y, Guo W, Pang J, Liu W, Fang Y, Wen X, Wei K, Gao X: Docetaxel loaded oleic acid-coated hydroxyapatite nanoparticles enhance the docetaxel-induced apoptosis through activation of caspase-2 in androgen independent prostate cancer cells. J Control Release 2010, 147: 278–288. 10.1016/j.jconrel.2010.07.108View Article
- Panyam J, Labhasetwar V: Dynamics of endocytosis and exocytosis of poly(D,L-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells. Pharm Res 2003, 20: 212–220. 10.1023/A:1022219003551View Article
- Yoo HS, Park TG: Folate-receptor-targeted delivery of doxorubicin nano-aggregates stabilized by doxorubicin-PEG-folate conjugate. J Control Release 2004, 100: 247–256. 10.1016/j.jconrel.2004.08.017View Article
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