Atomic force microscopy analysis of nanoparticles in nonideal conditions
 Petr Klapetek^{1}Email author,
 Miroslav Valtr^{1},
 David Nečas^{2},
 Ota Salyk^{3} and
 Petr Dzik^{3}
DOI: 10.1186/1556276X6514
© Klapetek et al; licensee Springer. 2011
Received: 15 October 2010
Accepted: 30 August 2011
Published: 30 August 2011
Abstract
Nanoparticles are often measured using atomic force microscopy or other scanning probe microscopy methods. For isolated nanoparticles on flat substrates, this is a relatively easy task. However, in real situations, we often need to analyze nanoparticles on rough substrates or nanoparticles that are not isolated. In this article, we present a simple model for realistic simulations of nanoparticle deposition and we employ this model for modeling nanoparticles on rough substrates. Different modeling conditions (coverage, relaxation after deposition) and convolution with different tip shapes are used to obtain a wide spectrum of virtual AFM nanoparticle images similar to those known from practice. Statistical parameters of nanoparticles are then analyzed using different data processing algorithms in order to show their systematic errors and to estimate uncertainties for atomic force microscopy analysis of nanoparticles under nonideal conditions. It is shown that the elimination of user influence on the data processing algorithm is a key step for obtaining accurate results while analyzing nanoparticles measured in nonideal conditions.
Introduction
Nanoparticle analysis is an important challenge in the present nanoscale metrology. Nanoparticles are used in many fields of research and technology [1–5], and their proper characterization is, therefore, very important. Even if there are several general and well established experimental methods to nanoparticle analysis (optical methods [6–8], electrochemistrybased methods [9], electron microscopy [10, 11], Xray methods [10, 12] and scanning probe microscopy [10, 13]), their results differ mutually very often due to different effects of nonideal measurement conditions [6, 7, 10, 14].
In this article, we focus on nanoparticle analysis performed using atomic force microscopy (AFM) [15], which is one of the most popular scanning probe microscopy methods. The interaction of nanoparticles with the AFM probe was studied in the past quite extensively from the experimental point of viewfrom the point of nanoparticle measurement, AFM tip modification, or nanoparticle manipulation [10, 13, 16–18]. If the isolated nanoparticles of spherical shape are deposited on an ideally flat substrate, their size can be determined easily from the AFM image by measuring the nanoparticle image height [13]. This quantity is not influenced by tipsample convolution effects and can provide accurate nanoparticle size results.
However, if the particles are deposited on rough substrates (or curved substrates generally), particle size analysis is not so straightforward and therefore many questions arise from the point of particle analysis implementation in AFM image processing software. Another effect strongly influencing the AFM analysis of nanoparticles is particle agglomeration and selfordering on the substrate. In real measurements, we can often observe both effects. The statistical results of nanoparticle properties therefore rely on a good choice and correct use of AFM data evaluation algorithms, which adds a human error to the whole measurement process. From a metrology point of view, this approach is not satisfactory, as we cannot easily determine the measurement uncertainty.
The aim of this article is to investigate the influence of the substrate roughness and particle agglomeration on the statistical analysis of nanoparticle properties. We study the measurement uncertainty of nanoparticle parameters with respect to different nanoparticle data processing methods and scanning parameters (e.g., tip related effects). To do this, we employ a simple model that simulates the real particle deposition and ordering on the substrate and the basic physical phenomena connected with this processes. Afterward, we simulate AFM scans obtained on modeled samples and we evaluate nanoparticle statistical properties using different data analysis methods. Finally, we compare the results with the nominal values of the nanoparticle statistical properties used for modeling on the first stage. This approach enables us to determine the level of confidence in AFM measurements of nanoparticles and to determine the limits of measurement uncertainty in these cases.
Experimental arrangement
Nanoparticle samples were prepared by spinoff coating, using a simple homebuilt apparatus; nanoparticles were dispersed in ethylene glycol [20] (palladium) resp. in water (polymer) and dried after deposition.
Data modeling and analysis
 1.
modeling of a rough surface.
 2.
simulation of particle deposition on the surface.
 3.
creation of virtual AFM images by tipsample convolution.
 4.
nanoparticle statistical analysis using virtual AFM images and data processing software.
In order to simulate the effects of both surface roughness and nanoparticle clustering, we need to vary the following parameters in steps 1 3:

surface roughness (parametrized using the root mean square roughness and autocorrelation length),

number of particles and their size (parametrized using the surface coverage and the particle radius),

AFM tip shape (parametrized using the tip radius and the apex ratio).
The resulting nanoparticle statistical properties are then compared to values used in step 2 (particle deposition). The algorithms used for data modeling are described in more detail in the next two sections. All the data modeling and processing algorithms were implemented in Gwyddion open source software http://gwyddion.net and are available for public in the present version of software.
Surface generation and particle deposition modeling
Rough substrates were modeled to have a Gaussian autocorrelation function [21], which is a simple model often used in many fields of surface physics [22].
where η (r) is the random number at the position given by r, η (r') is the random number at the position given by r' and d is the surface dimensionality (here d = 2).
where σ and/or T are the root mean square roughness and/or autocorrelation length corresponding to the surface to be constructed. As this integral is evaluated numerically, it is necessary to limit the computation to a sufficient area, depending on the decay of the Gaussian function inside the integral. The resulting values z(r) form a surface with the required root mean square roughness σ and autocorrelation length T.
Surface properties are therefore controlled by two parametersthe root mean square roughness (σ) and the autocorrelation length (T). Note that for our simulation, the autocorrelation length was kept constant and only the root mean square roughness was varied.
For particle deposition, a simple model similar to molecular dynamics calculations was constructed as described below. The aim of the model is to include basic interaction between nanoparticles and between a nanoparticle and the substrate and to model the effects of thermal and mechanical vibrations in nanoparticle dispersion (e.g., Brownian motion). In contrast to more rigorous models shown in literature [10, 23], this model does not include the effects of nanoparticle atomistic structure or effects of the presence of the vapor phase [24]; however, as seen from the results, it is still able to generate images of nanoparticles very similar to real images observed using atomic force microscopy (see Figure 1ad). In contrast to even simpler models (e.g., random placement of nanoparticles on substrate), it can include the nanoparticle selforganization effects, which are important phenomena affecting nanoparticle analysis in scanning probe microscopy as seen in Figure 1cd.
In order to model the nanoparticle deposition, we used the following algorithms and physical models:

Nanoparticles were modeled as LennardJones spheres, the surface by an integrated LennardJones potential [25, 26].

Verlet algorithm was used for the integration of the Newton equations.

The Anderson thermostat was used to simulate Brownian motion of nanoparticles in a liquid (which is the nanoparticle deposition in practice).

Nanoparticle velocities were damped during computation to simulate the decreasing mobility.
Virtual microscopy measurement and data evaluation
For the characterization of nanoparticles from AFM data, several algorithms can be used. The first stage is always the segmentation of data into separate particles. In principle, a simple thresholding can be used to do this for the case of isolated particles on a flat substrate. For rough or curved substrates and for agglomerated particles, this approach usually fails as we cannot determine an appropriate threshold value; therefore, we use a watershed approach for image segmentation in this article.
The watershed algorithm is a relatively simple alternative to thresholding, providing much better segmentation on complex structures [30, 31]. In this case, AFM data are first inverted in the zdirection. Virtual drops are then placed on the surface, leaving them to relax to minimum height, forming small "lakes." This is the first place (socalled location phase) after which the individual "lakes" are associated with a particle. After that, in the second phase (segmentation phase) the drops continue to be placed on the surface and relaxed, but unlike during the first phase, they are no longer allowed to merge. This leads to image segmentation. As a result, we obtain an image with marked individual grains.
 1.
algorithms based on nanoparticle projection on the xy plane. Even if this quantity can be highly affected by tip convolution, the effect of substrate curvature or particle agglomeration can be much smaller here than for heightbased algorithms. In this paper, this class of algorithms is represented by equivalent disk projection.
 2.
algorithms based on nanoparticle height or volume determination. For flat substrates and nonagglomerated particles, this class of algorithms leads to results that are not affected by tipsample convolution effects. For curved substrates, they can differ in the treatment of the surface beneath the particle, which cannot be observed using the AFM. In this paper, this class of algorithms is represented by two methodsvolume analysis using minimum boundary and Laplacian boundary method.
 3.
algorithms based on the statistical analysis of the whole measurement, e.g., autocorrelation function or power spectral density. These algorithms benefit from the selforganization of nanoparticles on substrate; in an ideal case, a honeycomb thin film is observed. In this work, power spectral density method is used.
There can be many varieties of details of the mentioned algorithms, providing slightly better or worse results in particular cases. In this article, we cannot discuss all of them. We aim to show the main trends in errors of different classes of algorithms under nonideal conditions in order to estimate how much we can trust their results.
For nanoparticle radii evaluation, the following algorithms were used (all based on their Gwyddion implementation, see http://gwyddion.net):

particle radius determined on the basis of equivalent disks (having the same area as the xy projection of particle). Here, the selected particle area (obtained from image segmentation, see above) is directly used as the particle crosssection. Particle volume is then calculated as volume of sphere having the same crosssection (see Figure 3b).

particle radius determined on the basis of its volume with respect to its boundary minimum. Here, the particle boundary is obtained from image segmentation, and the minimum height value along this boundary is used as a lower boundary to measure the particle volume (see Figure 3c). A numerical correction by a factor of 0.8 is employed to remove the apparent volume below the particle due to projection. The factor of 0.8 is the ratio between sphere volume and the volume of the sphere together with its cylindrical projection to the substrate.

particle radius determined on the basis of its volume with respect to Laplacian interpolation of its boundary. Here, the particle boundary is obtained by image segmentation, and Laplacian interpolation is run to obtain the morphology of the substrate below the particle (see Figure 3d). A numerical correction by a factor of 0.8 is employed to remove the apparent volume below particle due to projection.

particle radius determined by radial power spectral density evaluation. Here, a 2D Fast Fourier transform is performed from the whole image, and the particle size is determined from the observed maxima in the resulting radial power spectrum, which in the ideal case of closely packed nanoparticles are directly connected with the size of the nanoparticle.
As shown in the next section, each of the algorithms has its own benefits and drawbacks with respect to the treatment of nanoparticle agglomeration, tip size, or surface roughness.
Results and discussion
Typical examples of nanoparticle measurements are shown in Figure 1ad, representing palladium and polymer nanoparticles of different surface coverages, deposited on a flat silicon and a rough (anodically etched) silicon surface. We can see that both substrate roughness and particle agglomeration can be easily seen on the AFM images.
We have simulated several sets of nanoparticles on rough substrates with variable roughness (σ = 0 ÷ 10 nm). This range was chosen in order to include typical surface morphologies observed on surfaces and thin films prepared by different technological methods [32, 33]. Note that the surface root mean square value of 10 nm represents surface morphology with minimum to maximum range of some 100 nm, which is already a very high value (higher than the simulated nanoparticle size).
First, the nanoparticle coverage (ratio of sample area occupied by particles to total sample area) was varied, to include all the typical effects starting from isolated nanoparticles up to a substrate covered by several layers of nanoparticles. Secondly, the effects of relaxation and selfordering of nanoparticles were studied, simulating the nanoparticles with same coverages but different mobility and relaxation parameters during deposition modeling.
Nanoparticle radii results of nanoparticle radii simulated measurements for nanoparticles with nominal radius of 30 nm and different surface coverages.
σ= 0 nm  σ= 10 nm  

Pow  Min  Lap  Disk  Pow  Min  Lap  Disk  
C1, tip 1  32 ± 2  30 ± 1  30 ± 1  30 ± 1  33 ± 1  30 ± 3  30 ± 1  30 ± 1 
C1, tip 2  37 ± 7  34 ± 1  34 ± 1  44 ± 2  44 ± 20  33 ± 5  32 ± 4  41 ± 3 
C1, tip 3  N. A.  33 ± 3  33 ± 3  52 ± 5  N. A.  34 ± 6  34 ± 5  55 ± 9 
C2, tip 1  32 ± 1  30 ± 1  30 ± 1  29 ± 1  33 ± 2  30 ± 2  30 ± 1  30 ± 1 
C2, tip 2  38 ± 5  29 ± 3  25 ± 4  36 ± 3  38 ± 4  30 ± 5  28 ± 4  38 ± 6 
C2, tip 3  37 ± 6  28 ± 5  22 ± 6  38 ± 3  38 ± 8  26 ± 6  22 ± 7  38 ± 5 
C3, tip 1  30 ± 1  30 ± 1  30 ± 7  30 ± 1  31 ± 2  30 ± 2  28 ± 3  30 ± 1 
C3, tip 2  31 ± 3  31 ± 2  24 ± 6  34 ± 4  34 ± 2  28 ± 6  23 ± 7  34 ± 5 
C3, tip 3  34 ± 5  29 ± 5  21 ± 7  37 ± 5  33 ± 12  23 ± 8  17 ± 9  33 ± 7 
C4, tip 1  31 ± 1  N. A.  25 ± 4  29 ± 1  35 ± 5  29 ± 9  22 ± 8  28 ± 2 
C4, tip 2  30 ± 1  N. A.  19 ± 6  31 ± 3  31 ± 4  23 ± 12  18 ± 10  30 ± 8 
C4, tip 3  29 ± 1  N. A.  16 ± 5  36 ± 4  31 ± 4  N. A.  N. A.  30 ± 9 
We can discuss each algorithm performance more in detail separately, sorted by the amount of user influence on measurement results (which itself can affect the method reliability significantly):

Particle projection: particle projection evaluation method is in principle only as good as the segmentation used. Even if the watershed approach is very robust itself, in the presence of voids between particles, there is a need of fine tuning of the algorithm parameters in order to get an optimum segmentation. Moreover, this algorithm is the one most influenced by tip convolution effects as the particle projection changes significantly after convolution (namely for isolated particles).

Particle volumeboundary minimum basis: here, the influence of the tip convolution is smaller as the volume of particle changes relatively less than its projection. However, the location of the proper minimum on the particle boundary is crucial here and for densely packed particles, this algorithm fails. Here, the tip is no more able to reach the substrate at the voids between particles and detected boundary minimum is wrong, which leads to a distortion, namely for larger probes. Moreover, it can be expected that this algorithm is significantly affected by the local substrate slope (even for isolated nanoparticles) as the substrate slope is not employed in the evaluation (see Figure 3c).

Particle volumeLaplacian boundary interpolation basis: this approach treats the substrate geometry in an optimum way for isolated particles, and it can therefore provide slightly better results for high substrate roughnesses than the previous one. However, for densely packed particles or wide tips, it also fails, as the tip does not reach the substrate, similarly as in the previous case.

Power spectrum analysis: as for area analysis methods, this approach fails for small coverages and nonideal tips namely. As the tip convolution increases, the apparent width of the nanoparticles increases and there is no packing effect to block this. Resulting radii are much higher than would be expected. However, for dense packing this method seems to be quite robust, even for higher surface roughness. The main benefit of this method is that is does not use any segmentation; therefore, the amount of user influence is the smallest of all the discussed approaches.
As seen from the table, generally the particle volume based methods are only suitable for small coverages, where they produce reasonable results even for rough substrates. With higher packing of particles or smaller AFM tip side slope, the tip cannot reach the void volume between particles, which leads to a loss of information. Volume or height analysis methods are most sensitive to this effect.
For power spectrum and particle projection methods, the densely packed particles are an ideal measurand. However, even for isolated nanoparticles, these methods can be very effective if the AFM tip is sharp enough.
For all the methods based on image segmentation, the effect of the roughness can be highly suppressed by using a proper segmentation technique. Thresholding can be effective only for an extremely small roughness or substrate curvature. With a robust implementation of any more complex technique, e.g., the watershed algorithm, the effect of substrate irregularities can be highly suppressed.
As a real example, the selforganized nanoparticle film presented in Figure 1d was analyzed using all the mentioned algorithms. The nominal particle diameter of NIST traceable polymer nanoparticles was (46 ± 2) nm, i.e., the particle radius was 23 nm. They were deposited on a flat silicon substrate forming a film of unknown thickness and measured using a standard AFM tip. The resulting radii were (23 ± 3) nm for the equivalent disk radius method (particle projection), (11 ± 8) nm for the minimum basis grain volume method, (8 ± 8) nm for the Laplacean basis grain volume method and (24 ± 2) nm for the power spectrum analysis method. We can see that similarly to the modeling results, the particle projection and power spectrum method provide significantly better results for this type of sample.
Conclusion
In this article, the results of simulated nanoparticle measurements are presented. Nanoparticles are located on rough substrates, in some cases forming selforganized structures or even several layers. In this way, we simulate typical nonideal conditions observed at nanoparticle measurement using atomic force microscopy. To treat different tip convolution effects, nanoparticles are convolved with several typical AFM tip geometries. Results of different nanoparticle analysis algorithms are compared and discussed.
It is shown that for isolated nanoparticles, heightbased algorithms can be successful if the area below the nanoparticle is properly treated, both for flat and rough substrates, providing no systematic errors and uncertainties in the range of a few percents. However, for agglomerated nanoparticles or blunt AFM tips, these algorithms provide poor results and this effect is even worse for rough surfaces; in this case, the errors are comparable to estimated values.
For agglomerated particles, methods using lateral dimensions, both power spectrum based and particle projection based methods are very effective, even for rough substrates. These methods can provide results with uncertainty of a few percents and no systematic errors.
The worst case was observed for nonagglomerated nanoparticles with surface coverages between 30 ÷ 80%, where all classes of algorithms provide systematic errors and uncertainties larger than 10%. Here, a combination of all the approaches must be used and results must be interpreted very carefully.
It is shown that using a simple particle deposition modeling technique, together with a tipsample convolution algorithm, one can get relatively easily estimates of uncertainty components related to data processing methods in nanoparticle analysis. This can be also understood as a fast approach for uncertainty estimation in any particular case in practice. The method described is implemented in the open source software package for SPM data analysis Gwyddion http://gwyddion.net.
Declarations
Acknowledgements
This work was supported by the Grant Agency of the Czech Republic under contract 203/08/1594 and by EURAMET joint research project "Traceable measurement of nanoparticle size" funded from the European Union's Seventh Framework Programme, ERANET Plus, under Grant Agreement No. 217257.
Authors’ Affiliations
References
 Balamurugan A, Ho KC, Chen SM: Onepot synthesis of highly stable silver nanoparticlesconducting polymer nanocomposite and its catalytic application. Synth Met 2009, 159: 2544–2549. 10.1016/j.synthmet.2009.09.004View Article
 Brigger I, Dubernet C, Couvreur P: Nanoparticles in cancer therapy and diagnosis. Adv Drug Deliv Rev 2002, 54: 631–651. 10.1016/S0169409X(02)000443View Article
 Pradeep T, Anshup : Noble metal nanoparticles for water purification: A critical review. Thin Solid Films 2009, 517: 6441–6478. 10.1016/j.tsf.2009.03.195View Article
 Lee L, Zeng C, Cao X, Han X, Shen J, Xu G: Polymer nanocomposite foams. Compos Sci Technol 2005, 65: 2344–2363. 10.1016/j.compscitech.2005.06.016View Article
 Liu W: Nanoparticles and Their Biological and Environmental Applications. J Biosci Bioeng 2006, 102: 1–7. 10.1263/jbb.102.1View Article
 ZanettiRamos BG, FritzenGarcia MB, Schweitzer de Oliveira C, Pasa AA, Soldi V, Borsali R, CreczynskiPasa TB: Dynamic light scattering and atomic force microscopy techniques for size determination of polyurethane nanoparticles. Mat Sci Eng 2009, C 29: 638.View Article
 Bootz A, Vogel V, Schubert D, Kreuter J: Comparison of scanning electron microscopy, dynamic light scattering and analytical ultracentrifugation for the sizing of poly(butyl cyanoacrylate) nanoparticles. Eur J Pharm Biopharm 2004, 57: 369–375. 10.1016/S09396411(03)001930View Article
 Brar SK, Verma M: Measurement of nanoparticles by lightscattering techniques. Trends Anal Chem 2011, 30: 4–17. 10.1016/j.trac.2010.08.008View Article
 Yu L, Andriola A: Quantitative gold nanoparticle analysis methods: A review. Talanta 2010, 82: 869–875. 10.1016/j.talanta.2010.06.017View Article
 Henry CR: Morphology of supported nanoparticles. Prog Surf Sci 2005, 80: 92. 10.1016/j.progsurf.2005.09.004View Article
 Maurya A, Chauhan P: Structural and optical characterization of CdS/TiO2 nanocomposite. Mat Charact 2011, 62: 382–390. 10.1016/j.matchar.2011.01.014View Article
 RamalloLopez J, Giovanetti L, Craievich A, Vicentin F, JoseYacaman M, Requejo F: XAFS, SAXS and HREM characterization of Pd nanoparticles capped with nalkyl thiol molecules. Physica B 2007, 389: 150–154. 10.1016/j.physb.2006.07.044View Article
 Lee I, Chan KY, Phillips DL: Atomic force microscopy of platinum nanoparticles prepared on highly oriented pyrolytic graphite. Ultramicroscopy 1998, 75: 69. 10.1016/S03043991(98)000552View Article
 Lacava LM, Lacava BM, Azevedo RB, Lacava ZGM, Buske N, Tronconi AL, Morais PC: Nanoparticle sizing: a comparative study using atomic force microscopy, transmission electron microscopy, and ferromagnetic resonance. J Magn Magn Mat 2001, 225: 79. 10.1016/S03048853(00)012312View Article
 Binning G, Quate CF, Gerber C: Atomic force microscope. Phys Rev Lett 1986, 56(9):930–933. 10.1103/PhysRevLett.56.930View Article
 Theil Hansen L, Kühle A, Sørensen AH, Bohr J, Lindelof PE: A technique for positioning nanoparticles using an atomic force microscope. Nanotechnology 1998, 9: 337. 10.1088/09574484/9/4/006View Article
 Ong QK, Sokolov I: Attachment of nanoparticles to the AFM tips for direct measurements of interaction between a single nanoparticle and surfaces. J Colloid Interface Sci 2007, 310: 385. 10.1016/j.jcis.2007.02.010View Article
 Vakarelski IU, Brown SC, Moudgil BM, Higashitani K: Nanoparticleterminated scanning probe microscopy tips and surface samples. Adv Powder Technol 2007, 18: 605. 10.1163/156855207782514905View Article
 Klapetek P, Nečas D, Anderson C: Gwyddion user guide.[http://gwyddion.net]
 Bonet F, Delmas V, Grugeon S, Urbina RH, Silvert PY, TekaiaElhsissen K: Synthesis of monodisperse Au, Pt, Pd, Ru AND Ir nanoparticles in ethylene glycol. NanoStruct Mat 1999, 11(8):1277–1284. 10.1016/S09659773(99)004195View Article
 Yang HN, Zhao YP, Chan A, Lu TM, Wang GC: Samplinginduced hidden cycles in correlated random rough surface. Phys Rev B 1997, 56: 4224. 10.1103/PhysRevB.56.4224View Article
 Ogura H, Takahashi N: Scattering, Radiation and Propagation Over TwoDimensional Random Surface. PIER 1996, 14: 89.
 Ding L, Davidchack RL, Pan J: A molecular dynamics study of sintering between nanoparticles. Comp Mat Sci 2009, 45: 247. 10.1016/j.commatsci.2008.09.021View Article
 Rabani E, Reichman DR, Geissler PL, Brus LE: Dryingmediated selfassembly of nanoparticles. Nature 2003, 426: 271. 10.1038/nature02087View Article
 Campbellová A, Klapetek P, Valtr M: Tipsample relaxation as a source of uncertainty in nanoscale scanning probe microscopy measurements. Meas Sci Technol 2009, 20: 84014. 10.1088/09570233/20/8/084014View Article
 Yao H, Guduru P, Gao H: Maximum strength for intermolecular adhesion of nanospheres at an optimal size. J R Soc Interface 2008, 5: 1363. 10.1098/rsif.2008.0066View Article
 Villarubia JS: Algorithms for Scanned Probe Microscope Image Simulation, Surface Reconstruction, and Tip Estimation. J Res Natl Inst Stand Technol 1997, 102: 425.View Article
 FritzenGarcia MB, ZanettiRamos BG, de Oliveira CS, Soldi V, Pasa AA, CreczynskiPasa TB: Atomic force microscopy imaging of polyurethane nanoparticles onto different solid substrates. Mat Sci Eng C 2009, 29: 405–409. 10.1016/j.msec.2008.08.012View Article
 Tello M, Paulo AS, Rodríguez T, Blanco M, García R: Imaging cobalt nanoparticles by amplitude modulation atomic force microscopy: comparison between low and high amplitude solutions. Ultramicroscopy 2003, 97: 171–175. 10.1016/S03043991(03)00041XView Article
 Klapetek P, Ohlídal I, Franta D, MontaigneRamil A, Bonanni A, Stifter D, Sitter H: Atomic force microscopy characterization of ZnTe epitaxial films. Acta Phys Slovaca 2003, 53: 223.
 Gélinas V, Vidal D: Determination of particle shape distribution of clay using an automated AFM image analysis method. Powder Technol 2010, 203: 254. 10.1016/j.powtec.2010.05.016View Article
 Klapetek P, Ohlídal I, Navrátil K: Atomic Force Microscopy Analysis of Statistical Roughness of GaAs Surfaces Originated by Thermal Oxidation. Microchim Acta 2004, 147: 175–180.View Article
 Franta D, Ohlídal I, Klapetek P, Pokorný P: Characterization of the boundaries of thin films of TiO _{ 2 } by atomic force microscopy and optical methods. Surf Interface Anal 2002, 34: 759–762. 10.1002/sia.1405View Article
 Oikawa K, Kim H, Watanabe N, Shigeno M, Shirakawabe Y, Yasuda K: Measuring the sizes of nanospheres on a rough surface by using atomic force microscopy and a curvaturereconstruction method. Ultramicroscopy 2007, 107: 1061. 10.1016/j.ultramic.2007.03.011View Article
 ISO/IEC Guide 98–3:2008, Uncertainty of measurementPart 3: Guide to the expression of uncertainty in measurement (GUM:1995).
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