Drug-eluting Ti wires with titania nanotube arrays for bone fixation and reduced bone infection
© Gulati et al; licensee Springer. 2011
Received: 14 September 2011
Accepted: 31 October 2011
Published: 31 October 2011
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© Gulati et al; licensee Springer. 2011
Received: 14 September 2011
Accepted: 31 October 2011
Published: 31 October 2011
Current bone fixation technology which uses stainless steel wires known as Kirschner wires for fracture fixing often causes infection and reduced skeletal load resulting in implant failure. Creating new wires with drug-eluting properties to locally deliver drugs is an appealing approach to address some of these problems. This study presents the use of titanium [Ti] wires with titania nanotube [TNT] arrays formed with a drug delivery capability to design alternative bone fixation tools for orthopaedic applications. A titania layer with an array of nanotube structures was synthesised on the surface of a Ti wire by electrochemical anodisation and loaded with antibiotic (gentamicin) used as a model of bone anti-bacterial drug. Successful fabrication of TNT structures with pore diameters of approximately 170 nm and length of 70 μm is demonstrated for the first time in the form of wires. The drug release characteristics of TNT-Ti wires were evaluated, showing a two-phase release, with a burst release (37%) and a slow release with zero-order kinetics over 11 days. These results confirmed our system's ability to be applied as a drug-eluting tool for orthopaedic applications. The established biocompatibility of TNT structures, closer modulus of elasticity to natural bones and possible inclusion of desired drugs, proteins or growth factors make this system a promising alternative to replace conventional bone implants to prevent bone infection and to be used for targeted treatment of bone cancer, osteomyelitis and other orthopaedic diseases.
Kirschner wires [K-wires] are smooth stainless steel pins that have been widely used for temporary and definitive bone fixation, especially if the fracture fragments are small, e.g. wrist fractures and hand injuries . K-wires are generally passed through the skin, then transversely through the bone and out of the other side of the limb. This results in a potential passage for bacteria from the skin to migrate into the bone and cause an infection, referred to as pin tract infection . Such infections are generally caused by Staphylococcus aureus and Staphylococcus epidermidis which can adhere to the implant surface forming biofilms [2, 3]. These biofilms impair treatment and bone tissue healing as bacteria are protected from the antibiotics . Implant-associated infection is often treated with systemic administration of antibiotics and pin removal which compromises patient compliance and leaves fractures unfixed. If left unattended and unmanaged, this infection can lead to severe complexities like osteomyelitis, septic arthritis and similar problems . Also, it has been cited that with the use of external bone fixators, the infection rate can be as high as 33% . A possible solution to these problems is the coating of pins with antibiotics or to modify the implant surface to prevent such bacterial growth and infection [7, 8]. Another strategy is to replace such bone fixation stainless steel wires with another material where titanium, with regard to its proven biocompatibility, osseointegrating and superior mechanical properties, is an excellent choice .
Titania nanotube [TNT] arrays generated on a Ti surface by electrochemical anodisation have been extensively explored in the past several years for drug delivery systems, cell growth, biosensors and tissue engineering [10–13]. TNTs fabricated on a Ti implant surface can serve as carriers of drugs, proteins or growth factors for their localised delivery from an implant surface, which aid in reducing the incidence of infection or impaired bone healing [14–16]. Studies have established the capability of TNTs for local delivery of different therapeutics including water insoluble drugs, antibiotics and sensitive drugs such as proteins from the implant surface at the site of implantation [11, 14–17]. It was proven that the surface of antibiotic-loaded TNTs is capable of reducing bacterial adhesion whilst retaining the normal osteoblast adhesion and differentiation [18–20]. Studies from our group demonstrated several strategies to extend drug release from TNT implants which include controlling of nanotube structures, their surface modification, polymer coating and loading drugs into nanocarriers (polymer micelles) [21–23]. By coating TNT with biocompatible polymers such as poly(lactic-co-glycolytic acid) [PLGA] and chitosan, an extended release of water insoluble drugs up to more than 30 days and an improved adhesion proliferation of osteoblast cells were achieved .
Another advantage of using Ti is its lower modulus of elasticity, which matches more closely to that of the bone as compared with that of stainless steel K-wires. Hence, the skeletal load can be more evenly shared between the bone and the implant, resulting in a lower incidence of bone degradation due to stress shielding. Also, a TNT layer has a much closer elastic modulus to that of natural bones, and hence, it is expected to have a better biomechanical compatibility as compared with other implant materials . Thus, Ti with a TNT layer has a great potential promise in aiding enhanced bone healing and implant survival with minimised infection problems.
Titanium wire (99.7%) with a diameter of 0.75 mm was supplied by Alfa Aesar (MA, USA). Ethylene glycol, ammonium fluoride [NH4F] and gentamicin sulfate were obtained from Sigma-Aldrich (New South Wales, Australia). High purity Milli-Q water (Millipore Co., Billerica, MA, USA), ultra-pure grade (18.2 MΩ) and sieved through a 0.22- μm filter, was used.
The titanium wire was cut into a length of 2.5 cm, mechanically polished and cleaned by sonication in acetone for 30 min prior to anodisation. Two anodisation steps were performed using a specially designed electrochemical cell and computer-controlled power supply (Agilent Technologies Inc.) and a previously described procedure [27, 28]. In the first anodisation step, a constant voltage of 100 V was applied for 1 h in ammonium fluoride/ethylene glycol electrolyte (3% water and 0.3% NH4F) at a room temperature of 20°C. The resultant layer of anodic TNT layer was removed (by sonication in methanol), leaving the nanotextured titanium surface for the second anodisation. The second anodisation step to make the final TNT layer on Ti wire was performed using the same conditions. The voltage-current, voltage-time and current-time signals were adjusted and continuously recorded during the anodisation process by a software (Labview, National Instruments, Austin, TX, USA).
The structural characterisation of the prepared TNT/Ti wires before/after drug loading and drug release was performed using a field emission scanning electron microscope [SEM] (Philips XL 30, SEMTech Solutions, Inc., North Billerica, MA, USA). The samples were cut into small (approximately 5 mm) pieces, mounted on a holder with a double-sided conductive tape and coated with a layer of platinum 3 to 5 nm thick. Images with a range of scan sizes at normal incidence and at a 30° angle were acquired from the top, the bottom surface and the cross-sections.
A drug solution of 1% (w/v) gentamicin sulfate in water was prepared. Ti wires with a TNT surface were cleaned using deionised water and dried in nitrogen; 100 μl of the drug solution was pipetted onto the nanotube surface and allowed to dry in air. After drying, the TNT surface was using a soft tissue in order to remove excess drug accumulated on the surface. The wire was rotated after each step to ensure that the drug was loaded into nanotubes all around the wire. Loading, drying and wiping steps were repeated 20 times in order to load a substantial amount of drug into the nanotubes.
To determine the amount of drug loaded in the nanotubes, thermo-gravimetric analysis [TGA] was performed. In order to find the correct range of the drug decomposition, 20 to 25 mg of drug was loaded into the platinum pan in TGA and heated in the burning furnace from 20°C to 800°C, and its characteristic peak was obtained. Later, the drug-loaded TNTs were characterised, and the peak of the drug was identified in order to calculate the correct amount of drug present.
Drug release from the drug-loaded TNT-Ti wire samples was investigated by their immersion in 5 ml phosphate-buffered saline [PBS], where the amount of released drug was measured using ultraviolet-visible [UV-Vis] spectroscopy, as described previously . Measurements were taken at short intervals during the first 6 h to monitor the initial burst release, followed by testing every 24 h to observe the delayed release until the entire drug amount was released into the surrounding PBS. Absorbance was measured at 290 nm, and the corresponding drug concentration was calculated based on the calibration curve obtained for the drug. Ultimately, the release profiles of each experimental set were expressed for burst and delayed releases in a plot with release percentage vs. time. Drug release percentage (weight percentage) is calculated from the amount of drug released into the buffer solution, divided by the total amount of drug (weight) released at the end of the release (determined by UV-Vis spectrophotometer) and multiplied by 100.
The release characteristics of gentamicin from TNT-Ti
Drug release (%)
12.7 ± 1.2
36.2 ± 0.8
39.6 ± 0.5
48.5 ± 4.2
75.1 ± 13.6
100.0 ± 0.0
Weight release (μg)
25.4 ± 3.3
72.4 ± 1.4
79.2 ± 0.9
97.0 ± 5.8
150.2 ± 24.1
200 ± 0.1
In the second phase, different kinetics of drug release is observed from TNT-Ti with a very slow and linearly increased cumulative release over 11 days when no drug is detected inside the TNTs (Figure 4a). The release kinetics of this phase is controlled by a diffusion process from the deep nanotube structures (70 μm). For this stage, it is suggested that the gentamicin release mechanism is due to the diffusive transport through the ordered array of TNT since it is an insoluble matrix. Considering the high surface area and long capillary-like structures of TNTs, diffusion of the gentamicin drug to PBS can be described as a surface-dependent phenomenon. The TNT surface is negatively charged, and because the prominent chemical groups of gentamicin is aminoglycoside with amino groups (Figure 1) which are positively charged, an electrostatic interaction with the TNT surface could also have an influence on a long release observed on this drug.
The best fitting model for the gentamicin release data was observed using the Higuchi and zero-order releases, which describe drug release from an insoluble matrix . The square root of a time-dependent process is based on Fickian's diffusion law where diffusion-controlled release rate of drug molecules decreases as a function of time due to a reduction in the concentration gradient. The pharmaceutical dosage following the zero-order profile is the ideal method of drug release, providing the same amount of drug per unit of time. Our results confirmed that drug release into the local environment during this time was constant with a value of approximately 12 μg every day. By controlling the dimensions of TNT structures (diameter and length), this local concentration can be controlled and tuned to fit into an optimal therapeutic window for the treatment of bone infection by antibiotic. The general approach for antibiotic treatments through implantable devices requires a large drug loading and constant release over extended periods (e.g. weeks). To address this problem, we recently introduced several approaches to considerably extend drug release from TNT using polymer micelles and polymer coatings (plasma polymers, chitosan, PLGA) [23, 24]. These approaches can be applied here to achieve a long and sustained release of antibiotic with a desired concentration and zero-order kinetics over more than 4 weeks.
In our study, we report a new approach of preparing drug-eluting Ti implants in the form of Ti wires with a layer of TNT arrays fabricated as a bone fixative tool or an orthopaedic implant. A simple and cost-effective electrochemical technique was used for the synthesis of TNT arrays on Ti wire, followed by the loading of a common antibiotic drug, gentamicin. The drug loading and release of the model antibiotic drug (gentamicin) were characterised to reveal drug-eluting characteristics of our proposed implant. This system with TNT on Ti wires can be applied as a bone fixative tool, an implant or for complex bone ailments (for drug elution inside bones). The wire can easily be inserted inside the bones and could potentially open up new possibilities for enhanced bone fixation/repair and targeted treatment of bone cancer, osteomyelitis and other related orthopaedic diseases.
The authors acknowledge the financial support of the Australian Research Council (DP 0770930) and the University of South Australia for this work.
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