Shape engineering vs organic modification of inorganic nanoparticles as a tool for enhancing cellular internalization
© Sen Karaman et al.; licensee Springer. 2012
Received: 3 April 2012
Accepted: 23 May 2012
Published: 1 July 2012
In nanomedicine, physicochemical properties of the nanocarrier affect the nanoparticle's pharmacokinetics and biodistribution, which are also decisive for the passive targeting and nonspecific cellular uptake of nanoparticles. Size and surface charge are, consequently, two main determining factors in nanomedicine applications. Another important parameter which has received much less attention is the morphology (shape) of the nanocarrier. In order to investigate the morphology effect on the extent of cellular internalization, two similarly sized but differently shaped rod-like and spherical mesoporous silica nanoparticles were synthesized, characterized and functionalized to yield different surface charges. The uptake in two different cancer cell lines was investigated as a function of particle shape, coating (organic modification), surface charge and dose. According to the presented results, particle morphology is a decisive property regardless of both the different surface charges and doses tested, whereby rod-like particles internalized more efficiently in both cell lines. At lower doses whereby the shape-induced advantage is less dominant, charge-induced effects can, however, be used to fine-tune the cellular uptake as a prospective ‘secondary’ uptake regulator for tight dose control in nanoparticle-based drug formulations.
Keywordsmesoporous silica nanoparticles surface functionalization rod-shaped particles surface charge cellular internalization nanomedicine
Whereas nanotechnology seeks to engineer and apply the unique properties of materials that emerge when the dimensions enter the nanoscale, nanomedicine attempts to exploit these for the benefit of human health. Nanomaterials in this context are intriguing because they can resemble biological ‘nanomachines’ (such as biomacromolecules) as they meet on the same length scale and, thus, can be expected to perform similar tasks or at least possess reminiscent biobehavior. One of the properties exploited in nanomedicine is the ability of nanoparticles to enter cells, whereby they can function as efficient carriers for intracellular drug delivery. The behavior of nanomaterials on the nano-bio interface is largely determined by the physicochemical properties of the nanocarrier, of which size and surface charge have been emphasized as the two most critical ones. Another design parameter that has not received as much attention to date is particle shape. Available results suggest that, as compared to their spherical counterparts, elongated nanoparticles could be more favorable for therapeutic applications based on targeting specificity, biodistribution as well as cellular internalization profiles. On this topic, it has been suggested that the resemblance of rod-like particles with rod-like bacteria could be a reason for the observed advantages in internalization rates in non-phagocytic cells. Particles that, in such a way, mimic properties such as size, shape and flexibility of naturally occurring entities (for instance, circulating cells, e.g., red blood cells) may offer advantages that are typically not observed for standard (polymeric) particles.
One reason for the general scarcity of the shape-dependency effects on biological performance is the more difficult fabrication of nanoparticles with controlled rod-like morphology due to surface energy minimization during synthesis, leading to spherical shapes. Especially for more novel materials' classes, the first hurdles to overcome include obtaining well-dispersed, monosized particles throughout all processing steps. One promising materials' class in the sense of nanomedicine is mesoporous silica, which can be produced with nanoscale particle diameters and with tunable properties when it comes to both particle size and morphology, meso (pore) structure and size as well as surface chemistry. The controllable characteristics of these materials have boosted research regarding their potential use within the biomedical field during the last decade, especially so for targeted cancer therapy and diagnostics[9–11]. Mesoporous silica nanoparticles (MSNs) have been successfully loaded with a range of different chemotherapeutics for efficient intracellular delivery and subsequent elimination of the cancer cells. A vast array of sizes and surface modifications have been produced and studied both in vitro and in vivo, while only a few studies have been devoted to the shape effect on MSN biobehavior[12–16]. To add to the increased understanding of shape-induced effects for MSNs, we have, in this study, synthesized two similarly sized but differently shaped rod-like (NR-MSP) and spherical (S-MSP) fluorescently labeled mesoporous silica nanoparticles in order to investigate the morphology effect on cellular internalization. Additionally, both the rods and spheres were functionalized to yield different surface charges in order to distinguish morphology from surface charge-induced effects and, in the best case scenario, find the dominant parameter. In vitro studies were carried out in two different cancer cell lines, HeLa (cervical carcinoma cells) and Caco-2 (human epithelial colorectal adenocarcinoma cells), to investigate the particle characteristic impact on cells of different origin. According to our results, all of the studied factors (particle shape, surface charge, the nature of the coating as well as the targeted cell populations) are important to be considered when designing new nanocarrier formulations for targeted cancer therapies or other potent drugs that require tight dose control.
Particle preparation and characterization
Spherical mesoporous silica particles
S-MSP1 was synthesized according to the protocol described in reference, with slight modifications and details given in Additional file1. In order not to alter the surface charge of the particles, fluorescein isothiocyanate (FITC)-modified aminopropyltriethoxysilane silane (APTES) was mixed with the silica source before adding to the reaction solution to provide co-condensed functionalization of FITC within the silica framework. The modification of APTES was carried out by pre-reacting FITC with APTES in 2 mL acetone with a molar ratio of 1:3 and stirring for 2 h under inert atmosphere. The molar ratio between APTES and tetraethyl orthosilicate (TEOS) was kept as 1:100. The thus-preserved negative surface charge was subsequently utilized for further electrostatic adsorption of branched 25 k poly(ethylene imine) (PEI) to the surfactant-extracted particles (S-MSP1-PEI adsorbed). The functionalization of S-MSP1 was carried out by overnight adsorption (Figure 2) in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer at neutral pH, after which, the particles were collected by centrifugation and washed excessively with deionized water to remove excess PEI.
S-MSP2 was synthesized according to our previously published protocols, with FITC added in the synthesis step to create inherently fluorescent particles. In this case, no pre-reaction between the aminosilane and FITC was needed as the amount of aminosilane used in the synthesis was considerable (10 mol%), and the reaction conditions are favorable also for FITC conjugation. As the surface charge of the MSPs prepared according to this protocol is neutral to slightly positive, electrostatic adsorption of PEI is not possible. Thus, these particles were PEI-functionalized by surface growing of PEI[19–21] to yield sample S-MSP2-PEI grafted (Figure 2).
Rod-like mesoporous silica particles
NR-MSPs were synthesized according to reference, with TEOS as silica source and block-co-polymer P123 (EO20PO70EO20) as pore-structuring agent in the presence of NH4F and heptane. Contrary to the S-MSP syntheses, the nanorod (NR) synthesis is performed under acidic conditions, where the particle length and width can be tuned with the aid of HCl concentration. The synthesis solution consisted of a molar ratio of 1 P123/1.8 NH4F/280 Heptane/60 TEOS/356 HCl/10335 H2O. The synthesis was kept under vigorous stirring for 4 min and subsequently, under static conditions for 1 h. Then, the solution was transferred to a closed Teflon flask for hydrothermal treatment at 100°C for 24 h. The resulting product was filtered and washed with distilled water. Finally, the material was calcined at 550°C for 5 h in order to remove the structure-directing agent (SDA) P123.
The produced nanorods were subsequently fluorescently labeled post-synthesis using the same pre-reaction solution as was added already in the synthesis step for S-MSP1. Thus, the negative surface charge of the NR-MSPs could also be preserved, and as a comparison to both S-MSPs, the NR-MSPs were PEI-functionalized both by surface grafting (NR-MSP-PEI graf.) and electrostatic adsorption (NR-MSP-PEI ads.). Furthermore, to investigate the surface charge effect of NR-MSPs, the NR-MSP-PEI graf. samples were further functionalized via either succinylation to yield negatively charged succinic acid groups[21, 23] (NR-MSP-PEI-SUCC) or capping of the primary amines with uncharged acetyl groups (NR-MSP-PEI-ACA). For both functionalization regimes, the PEI-functionalized particles were dispersed in DMF, into which either succinic or acetic anhydride was added in excess. The reaction suspension was agitated overnight. Next day, the particles were separated by centrifugation, washed with absolute ethanol and vacuum dried or directly dispersed into dimethyl sulfoxide (DMSO) at a concentration of 5 mg/ml for cellular experiments.
Particle characterization methods
Thermogravimetrical analysis (Netzsch TG 209) was used to determine the amount of PEI added at temperature intervals of 170°C to 770°C. Successful modification of PEI and further derivatization with succinic acid or acetyl groups were further confirmed by zeta potential measurements (Malvern ZetaSizer NanoZS, Malvern Instruments Ltd., Worcestershire, UK). Full redispersibility of dried, extracted and surface-functionalized particles was confirmed by redispersion of dry particles in HEPES buffer at pH 7.2 and subsequent dynamic light scattering (DLS) measurements (Malvern ZetaSizer NanoZS). Scanning electron microscopy (SEM; Jeol JSM-6335 F (JEOL Ltd., Tokyo, Japan) for S-MSPs and Leo 1550 Gemini SEM (Zeiss, Oberkochen, Germany) for NR-MSPs) further confirmed the size, monodispersity, morphology and non-agglomerated state of the particles. The mesoscopic ordering of the nanoparticles was further confirmed by transmission electron microscopy (FEI Tecnai 12 TEM (FEI Co., Hillsboro, OR, USA) operating at 120 kV with a LaB6 filament and a 2 k × 2 k CCD camera) as well as powder XRD using a Kratky compact small-angle system (MBraun, Nottinghampshire, UK). The structural parameters related to the mesoporosity (surface area, pore size and pore volume) were determined by nitrogen sorption measurements (ASAP 2020 (Micromeritics Instrument Corp., Norcross, GA, USA) for NR-MSPs and Autosorb 1 (Quantachrome, Boynton Beach, FL, USA) for S-MSPs). Successful incorporation of fluorescein was determined by fluorescence spectrometry (Perkin Elmer LS 50B, PerkinElmer, Waltham, MA, USA) of particles dispersed in HEPES at a concentration of 0.5 mg/ml by excitation at 490 nm and determining the fluorescence intensity at wavelength 520 nm.
Cell culturing, fluorescence-assisted cell sorting and cytotoxicity
HeLa cells and Caco-2 cells obtained from ATCC (Manassas, VA, USA) were maintained in DMEM medium (Sigma, St. Louis, MO, USA) supplemented with 10% fetal calf serum (BioClear, Wiltshire, UK), 2 mM L-glutamin, 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in a 5% CO2/95% O2 and 90% RH humidified atmosphere and handled under sterile conditions.
Cellular uptake by fluorescence-assisted cell sorting and confocal fluorescence microscopy
MSPs were suspended in a cell medium at different concentrations (1, 2 and 10 μg/ml) according to details given in Additional file1. The amount of endocytosed particles inside the cells was analyzed by BD FacsCalibur flow cytometer (FL-I, BD Pharmingen, San Jose, CA, USA). The mean fluorescence intensity (MFI) of the cells at FL-1 channel was measured. The data were analyzed with BD CellQuest Pro™ software for the total amount of MSP uptake by 10,000 cells. GraphPad Prism 5.0 software was used for the statistical analysis of the results. The bar graphs in the figures represent mean values (±SD) from four or more independent experiments.
For microscopical studies, HeLa cells were seeded on a glass-bottom chamber slide (Lab-Tek™, Brendale, Australia) and incubated as explained in Additional file1. The cells were viewed with Leica TCS SP5 confocal microscope (Leica Micosystems, Wetzlar, Germany; ×63 oil objective, 488 nm/514 nm/543 nm excitation).
Cell viability by WST-1 assay
HeLa cells were transferred to 96-well plates (9,000 cells/well) and allowed to attach and grow. After 24 h, the medium was removed and replaced with 100 μl medium containing different concentrations of MSPs (10 and 25 μg/ml). After incubation for 12 h, 10 μl of WST-1 (Roche Applied Science, Upper Bavaria, Germany) reagent was added to the cells, and further incubated for 90 min; after which, the 96-well plate was analyzed at a 430-nm wavelength in a Varioskan plate reader (Thermo Scientific, Logan UT, USA) to determine cell viability. Here, negative control (NC) is the cell media only without particles, NC DMSO-cell is the cell media with particle vehicle (DMSO), and positive control (PC) is with calyculin A (50 ng/ml) added to the cell media.
Characteristics of silica nanoparticle suspensions (0.5 mg/ml) in HEPES buffer (25 mM, pH 7.2)
Hydrodynamic Size (nm)
Zeta potential (mv)
Fluorescence intensity at λ = 520 nm
The fluorescent labeling of the particles regardless of method (co-condensation or post-synthesis grafting) were successful, as determined by fluorescence intensity measurements in HEPES buffer at the same concentrations (0.5 mg/ml; Table1). On a related note, we want to highlight the alteration of fluorescence intensity upon surface functionalization when fluorescein is used as the fluorescent tag. Fluorescein, mostly used in the ‘activated’ form FITC, is a pH probe, and thus, both its absorption and fluorescence properties vary with pH as well as solvent. This not only has implications for its intracellular localization upon quantification of cellular uptake as the pH in different cellular compartments differ, and for instance, in endo/lysosomes where particles would reside as a result of endocytotic uptake, the environment is acidic (pH 5 to 6). As is clearly illustrated in Table1, surface functionalization, especially with acidic/basic groups such as amines or carboxylic acids (PEI and SUCC) which are the mostly used derivatizations when further bioconjugation is aimed for, also results in local pH changes on the nanoparticle surface. This has direct implications for differences in fluorescence intensity of the nanoparticles, despite being based on the same particle and measured at the same pH (here, in HEPES buffer at pH 7.2). This complicates fluorescence intensity-based quantification of cellular uptake as intracellular localization of the particles is not known at the time of detection whereby fluorescence intensity could also be measured at the right pH, and moreover, the localization for all particles within the same cell is not necessarily coinciding. Nevertheless, despite this drawback, fluorescein is the most widely used fluorescent tag due to its low cost, and these limitations in the absolute quantification based solely on fluorescence intensity are rarely taken into account. Consequently, we have chosen to mainly focus on percentage of positive cells in our cellular uptake studies as this value is not dependent on the absolute intensity of the particles.
Cellular uptake of uncoated particles
Cellular uptake of organically modified particles
Organic modification is almost exclusively required to obtain desirable properties for a MSN system in order to provide for eligible biobehavior. The added value in organic modification may include dispersion stability, surface charge tuning, stealth properties, anchoring points for biofunctionalization (antibodies, proteins, peptides, targeting ligands and drug molecules), increased hydrophilicity, molecular gate-keeping, stimuli responsiveness, controlled cleavage of surface-bound coating or moieties, and so forth. On a less sophisticated level, it is generally believed that a net positive charge is beneficial in maximizing cellular uptake due to attraction to the negatively charged cell membrane. Thus, functionalization with positively charged surface groups is frequently applied to enhance cellular uptake. This is also the primary reason why we decided to functionalize our particles with the cationic polyelectrolyte PEI. Aside from enhanced cellular uptake, the branched structure of PEI also offers a higher amount of terminal primary amino groups than possible to attain via conventional amino functionalization, simultaneously providing increased electrostatic suspension stability to the system and possibly introducing pH-dependent molecular gate properties along with enabling endosomal escape ability, both of which would be utilized when carrying drug cargo for intracellular release. To investigate the effect on cellular uptake upon derivatization of the PEI layer, we also capped the terminal primary amino groups with either uncharged acetyl groups or acidic (negatively charged under neutral conditions) succinic acid groups. The uptake in terms of positive cell percentage of the whole series of particles with (or without) different functionalization is presented in Figure 7.
Our observed enhanced uptake of the rod-like MSPs is in line with previous reports on rod-like MSN biobehavior. These previous reports have mainly concentrated on non-functionalized MSNs aside from the fluorescent labeling (i.e., comparable to our S-MSP1 and NR-MSP) to render the particles observable by confocal (fluorescence) microscopy and other applied fluorescence-based characterization techniques. Meng et al. investigated the aspect ratio effect on cellular uptake rate and amount, and found that particles with an AR of 2.1 to 2.5 were taken up in greater quantities than shorter (1.5 to 1,7) and longer (4 to 4.5) rods as well as spheres (AR = 1), which seem consistent with earlier reports on organic nanoparticles for which AR ~ 3 was found to be the most efficient in terms of cellular internalization. The aspect ratio of our studied NR-MSP is around 3 to 4. Meng et al. stated that their observations implied that a cellular mechanism capable of discerning and responding to rod length was operative, and compared this observation to that of Champion and Mirtagotri, who studied the phagocytosis mechanism of non-spherical polymer particles in macrophages and concluded that it was the local geometry (angle of curvature) at the point of contact with the cell membrane that initiated phagocytosis, not the overall AR. It is worth to mention that the two mechanisms of uptake studied in the two separate studies were different, though Meng et al. studied macropinocytosis and the effect of particle shape on this particular cellular uptake route in two cancer cell lines (HeLa and A549). Huang et al. also studied the effect of MSNs of different ARs (1, 2 and 4) on uptake amounts and rates in a human melanoma (A357) cell line as well as AR impacts on cellular function including cell proliferation, apoptosis, cytoskeleton formation, adhesion and migration. They found that increasing AR gave rise to increased uptake whereas long rod-shaped particles could cause reorganization of the cytoskeleton, and ascribed this observation to the more efficient endocytosis of rod MSNs than spheres. Whereas Meng et al. found the behavior to be similar between the two cancer cell lines studied, Trewyn et al. found slight differences between cell lines when comparing a cancer cell line (Chinese hamster ovarian) to a human fibroblast cell line. In our current investigation between two cancer cell lines, the overall trends seem to be similar but with different dose-dependencies. Huang et al. studied the effect of uncoated and PEGylated MSNs with two different ARs (1.5 and 5) on biodistribution, clearance and biocompatibility in vivo, whereby they found that the AR affected the clearance rate in that short rod MSNs (AR ~ 1.5) had a more rapid clearance rate than the long rod MSNs (AR ~ 5). No profound in vivo toxicity (based on hematology, serum biochemistry and histopathology) was found, in line with their previous report where MSN concentrations as high as 1,000 mg/kg were used. Another useful means of predicting in vivo compatibility when aiming for circulating nanoparticles is determining their hemolytic activity, whereby Yu et al. have investigated pure and amino-functionalized porous and non-porous silica nanoparticles of ARs 2, 4 and 8 and their cytotoxicity effects on macrophages (RAW 264.6), lung carcinoma cells (A549) and hemolysis of human erythrocytes. The pure silica particles showed a porosity- and shape-dependency on hemolytic activity, with the porous MSNs with higher AR exhibiting reduced hemolytic activity, whereas for the amino-functionalized particles, the zeta potential (effective surface charge) was decisive. On this note, we want to stress that as we have particles of distinctively different pore sizes (3 to 4 nm vs 11 to 12 nm), this might give rise to differences in hemolytic activity as it has also been pointed out earlier that porosity is a decisive property due to lower exposure of silanol groups to the cell membrane of a porous surface. However, as surface modification eliminates this source of interaction, the porosity effect might be of subordinate importance for surface-modified MSPs. In the study by Yu et al., surface charge and porosity also governed cellular toxicity, whereas AR did not have any reported effect. Also in our current study, no differences in cytotoxicity were observed at doses relevant for our experiments and higher (2.5 times our maximum used dose). We also note that the two different pore sizes of our chosen MSPs are rationalized based on applicability for different types of therapeutic cargo, whereby the 3- to 4-nm pores are ideal for the loading of small-molecular drugs, whereas the large-pore materials would also be suitable for carrying of biomacromolecules such as proteins (antibodies, enzymes, polypeptides and so on) and genes[37, 38]. Finally, based on the troublesome nature of the fluorescein label, a more quantitative/absolute approach to studying the shape effect on nanoparticle uptake with nanomedical prospects and the efficiency of different particles in cargo delivery could constitute a more correlative approach. To date, this has been successfully measured in the extent of causing cytotoxicity upon delivery of the chemotherapeutic agent camptothecin or paclitaxel or the efficacy of GFP knockdown upon siRNA delivery, and similar approaches will also be pursued in our ongoing and future studies.
Porous silica particles of spherical and rod-like morphologies were studied for cellular uptake efficiency in two different cancerous cell lines for potential applications as nanomedical drug delivery carriers. According to the obtained results, both rod-like and spherical particles were readily internalized by HeLa cells with slight shape and charge-induced differences, whereas in Caco-2 cells, rod-shaped particles were internalized more efficiently. The difference was most pronounced for uncoated particles in both cell lines, whereby higher charge (S-MSP1) also induced higher uptake. A net positive charge (PEI) enhanced uptake regardless of shape and cell line. At lower doses, surface charge could be used to fine-tune the uptake even in HeLa cells, whereby higher charge (+/−) results in higher uptake over net neutral charge and positive over negative. At higher concentrations, the surface charge effect is overridden in HeLa cells, and rods are taken up despite coating or not, whereas for spheres and Caco-2 cells, distinctions can still be made. Uptake studies performed in vitro in different cell lines show that along with particle shape and surface functionalization, cellular origin and features may also influence the uptake of particles in cells. As shape seems to influence uptake in a cell-dependent manner, shape engineering could potentially be used as a tool for enhancing nanoparticle-mediated delivery.
The Academy of Finland projects (#140193 JMR; #140759 and #126161, DMT; #137101, JMR, DD and NR), Centre of Excellence for Functional Materials (DSK), Centre for International Mobility India-Finland Fellowship (DD), Sigrid Juselius Foundation (RS, DMT), Nanolith Sverige AB (EJ and MO), FP7 IRG (DMT), ÅAU Center of Excellence (DMT) and Liv och Hälsa Foundation (DMT) are acknowledged for the financial support. Helena Saarento, Perttu Terho and Jari Korhonen are acknowledged for their technical assistance.
- Niemeyer CM: Nanoparticles, proteins, and nucleic acids: biotechnology meets materials science. Angew Chem Int 2001, 40: 4128–4158. 10.1002/1521-3773(20011119)40:22<4128::AID-ANIE4128>3.0.CO;2-SView Article
- Mitagotri S: In drug delivery, shape does matter. Pharmaceutical Research 2009, 26(1):232–234. 10.1007/s11095-008-9740-yView Article
- Venkataraman A, Hedrick JL, Ong ZY, Yang C, Rachel Ee PL, Hammond PT, Yang YY: The effects of polymeric nanostructure shape on drug delivery. Adv Drug Delivery Rev 2011, 63: 1228–1246. 10.1016/j.addr.2011.06.016View Article
- Gratton SEA, Ropp PA, Pohlhaus PD, Luft JC, Madden VJ, Napier ME, DeSimone JM: The effect of particle design on cellular internalization pathways. PNAS 2008, 105(33):11613–11618. 10.1073/pnas.0801763105View Article
- Mitragotri S: Designer polymer particles for drug delivery [abstract]. In Tools for ADMET and Pharmaceutical Nanotechnology: September 18–20 2011; Helsinki. 2011. Helsinki: University of Helsinki; 2011.
- Decuzzi P, Pasqualini R, Arap W, Ferrari M: Intravascular delivery of particulate systems: does geometry really matter? Pharmaceutical Research 2009, 26(1):235–243. 10.1007/s11095-008-9697-xView Article
- Rosenholm JM, Sahlgren C, Lindén M: Towards intelligent, targeted drug delivery systems using mesoporous silica nanoparticles – opportunities & challenges. Nanoscale 2010, 2: 1870–1883. 10.1039/c0nr00156bView Article
- Popat A, Hartono SB, Stahr F, Liu J, Qiao SZ, Lu GQ: Mesoporous silica nanoparticles for bioadsorption, enzyme immobilization, and delivery carriers. Nanoscale 2011, 3: 2801–2818. 10.1039/c1nr10224aView Article
- Li Z, Barnes JC, Bosoy A, Stoddart JF, Zink JI: Mesoporous silica nanopartilces in biomedical applications. Chem Soc Rev 2012. 10.1039/c1cs15246g
- Lee JE, Lee N, Kim T, Kim J, Hyeon T: Multifunctional mesoporous silica nanocomposite nanoparticles for theranostic applications. Acc Chem Res 2011, 44(10):893–902. 10.1021/ar2000259View Article
- Rosenholm JM, Mamaeva V, Sahlgren C, Lindén M: Nanoparticles in targeted cancer therapy: mesoprous silica nanopartilces entering preclinical development stage. Nanomedicine 2012, 7: 111–120. 10.2217/nnm.11.166View Article
- Huang X, Teng X, Chen D, Tang F, He J: The effect of the shape of mesoporous silica nanoparticles on cellular uptake and cell function. Biomaterials 2010, 31: 438–448. 10.1016/j.biomaterials.2009.09.060View Article
- Meng H, Yang S, Li Z, Xia T, Chen J, Ji Z, Zhang H, Wang X, Lin S, Huang C, Zhou ZH, Zink JI, Nel AE: Aspect ratio determines the quantity of mesoporous silica nanoparticle uptake by a small GTPase-dependent macropinocytosis mechanism. ACS Nano 2011, 5(6):4434–4447. 10.1021/nn103344kView Article
- Huang X, Li L, Liu T, Hao N, Liu H, Chen D, Tang F: The shape effect of mesoporous silica nanoparticles on biodistribution, clearance, and biocompatibilty in vivo. ACS Nano 2011, 5(7):5390–5399. 10.1021/nn200365aView Article
- Yu T, Malugin A, Ghandehari H: Impact of silica nanoparticle design on cellular toxicity and hemolytic activity. ACS Nano 2011, 5: 5717–5728. 10.1021/nn2013904View Article
- Trewyn BG, Nieweg JA, Zhao Y, Lin VSY: Biocompatible mesoporous silica nanoparticles with different morphologies for animal cell membrane penetration. Chem Eng J 2008, 137: 23–29. 10.1016/j.cej.2007.09.045View Article
- He Q, Shi J, Chen F, Zhu M, Zhang L: An anticancer drug delivery system based on surfactant-templated mesoporous silica nanoparticles. Biomaterials 2010, 31(12):3335–3346. 10.1016/j.biomaterials.2010.01.015View Article
- Rosenholm JM, Meinander A, Peuhu E, Niemi R, Eriksson JE, Sahlgren C, Lindén M: Targeting of porous hybrid silica nanoparticles to cancer cells. ACS Nano 2009, 3(1):197–206. 10.1021/nn800781rView Article
- Rosenholm JM, Penninkangas A, Lindén M: Amino-functionalization of large-pore mesoscopically ordered silica by a one-step hyperbranching polymerization of a surface-grown polyethyleneimine. Chem Commun 2006, 37: 3909–3911.View Article
- Rosenholm JM, Lindén M: Wet-chemical analysis of surface concentration of accessible groups on different amino-functionalized mesoporous SBA-15 silicas. Chem Mater 2007, 19: 5023–5034. 10.1021/cm071289nView Article
- Rosenholm JM, Duchanoy A, Lindén M: Hyperbranching surface polymerization as a tool for preferential functionalization of the outer surface of mesoporous silica. Chem Mater 2008, 20: 1126–1133. 10.1021/cm7021328View Article
- Johansson EM, Ballem MA, Córdoba JM, Odén M: Rapid synthesis of SBA-15 rods with variable lengths, widths, and tunable large pores. Langmuir 2011, 27: 4994–4999. 10.1021/la104864dView Article
- Bergman L, Rosenholm JM, Öst AB, Duchanoy A, Kankaanpää P, Heino J, Lindén M: On the complexity of electrostatic suspension stabilization of functionalized silica nanoparticles for biotargeting and –imaging applications. J Nanomaterials 2008. 10.1155/2008/712514
- Zintchenko A, Philipp A, Dehshahri A, Wagner E: Simple modifications of branched PEI lead to highly efficient siRNA carriers with low toxicity. Bioconjugate Chem 2008, 19: 1448–1455. 10.1021/bc800065fView Article
- Johansson EM, Córdoca JM, Odén M: Effect of heptane addition on pore size and particle morphology of mesoporous silica SBA-15. Microporous Mesoporous Mater 2010, 133: 66–74. 10.1016/j.micromeso.2010.04.016View Article
- Li M, Wang N, Liang Y, Zhang J: Preparation of monodisperse short rod-like mesoporous silica. Chinese J Mat Res 2006, 20(2):181–185.
- Selvan ST, Aldeyab SS, Zaidi JSM, Arivuoli D, Ariga K, Mori T, Vinu A: Preparation and characterization of highly ordered mesoporous SiC nanoparticles with rod shaped morphology and tunable pore diameters. J Mater Chem 2011, 21: 8792–8799. 10.1039/c1jm10545kView Article
- Cook A, Le A: The effect of solvent and pH on the fluorescence excitation and emission spectra of solutions containing fluorescein. J Phys Chem Lab 2006, 10: 44–49.
- Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP: A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A 1995, 92(16):7297–7301. 10.1073/pnas.92.16.7297View Article
- Sahlin S, Hed J, Rundquist I: Differentation between attached and ingested immune complexes by a fluorescence quenching cytofluorometric assay. J Immunoll Methods 1983, 60: 115–124. 10.1016/0022-1759(83)90340-XView Article
- Rosenholm JM, Peuhu E, Eriksson JE, Sahlgren C, Lindén M: Targeted intracellular delivery and release of hydrophobic agents using mesoporous hybrid silica nanoparticles as drug carrier systems. Nano Letters 2009, 9: 3308–3311. 10.1021/nl901589yView Article
- Choi HS, Liu W, Misra P, Tanaka E, Zimmer JP, Kandapallil B, Bawendi MG, Frangioni JV: Renal clearance of quantum dots. Nat Biotechnol 2007, 25: 1165–1170. 10.1038/nbt1340View Article
- Verma A, Stellacci F: Effect of surface properties on nanoparticle-cell interactions. Small 2010, 6: 12–21. 10.1002/smll.200901158View Article
- Champion JA, Mitragotri S: Shape induced inhibition of phagocytosis of polymer particles. Pharmaceutical Research 2009, 26(1):244–249. 10.1007/s11095-008-9626-zView Article
- Liu TL, Li LL, Teng X, Huang XL, Liu HY, Chen D, Ren J, He JQ, Tang FQ: Single and repeated dose toxicity of mesoporous hollow silica nanoparticles in intravenously exposed mice. Biomaterials 2011, 32: 1657–1668. 10.1016/j.biomaterials.2010.10.035View Article
- Lin YS, Haynes CL: Impacts of mesoporous silica nanoparticle size, pore ordering, and pore integrity on hemolytic activity. JACS 2010, 132(13):4834–4842. 10.1021/ja910846qView Article
- Rosenholm JM, Sahlgren C, Lindén M: Multifunctional mesoporous silica nanoparticles for combined therapeutic, diagnostic and targeted action in cancer treatment. Current Drug Targets 2011, 12(8):1166–1186. 10.2174/138945011795906624View Article
- Rosenholm JM, Zhang J, Sun W, Gu H: Large-pore mesoporous silica-coated magnetite core-shell nanocomposites and their relevance for biomedical applications. Microporous Mesoporous Materials 2011, 145(1–3):14–20.View Article
- Kolhar P, Doshi N, Mitragotri S: Polymer nanoneedle-mediated intracellular drug delivery. Small 2011, 7(14):2094–2100. 10.1002/smll.201100497View Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.