Light-harvesting bio-nanomaterial using porous silicon and photosynthetic reaction center
© Hajdu et al.; licensee Springer. 2012
Received: 30 April 2012
Accepted: 19 June 2012
Published: 17 July 2012
Porous silicon microcavity (PSiMc) structures were used to immobilize the photosynthetic reaction center (RC) purified from the purple bacterium Rhodobacter sphaeroides R-26. Two different binding methods were compared by specular reflectance measurements. Structural characterization of PSiMc was performed by scanning electron microscopy and atomic force microscopy. The activity of the immobilized RC was checked by measuring the visible absorption spectra of the externally added electron donor, mammalian cytochrome c. PSi/RC complex was found to oxidize the cytochrome c after every saturating Xe flash, indicating the accessibility of specific surface binding sites on the immobilized RC, for the external electron donor. This new type of bio-nanomaterial is considered as an excellent model for new generation applications of silicon-based electronics and biological redox systems.
KeywordsPorous silicon functionalization Peptide Photosynthetic reaction center Nanomaterial Biophotonics
In the last few years, the use of bio-nanocomposites has been the subject of extensive study. Using a hybrid material, it may be possible to harness the advantages of two different materials at the same time. Several attempts to fabricate functional biocomposites by different groups have been reported [1–6]. Photosynthetic reaction center (RC) is one of the proteins of high interest, because it is nature's solar battery, converting light energy into chemical potential in the photosynthetic membrane, thereby assuring carbon reduction in cells [7, 8]. Although RC functions on the nanometer scale, with nanoscopic power, this is the protein that assures the energy input practically for the whole biosphere on Earth. The extremely large quantum yield of the primary charge separation (close to 100%)  in RC presents a great challenge to use it in artificial light harvesting systems. However, as biological materials are very sensitive to the external effects and are generally stable only in their own environment, to keep them functional after their isolation, a special vehicle is necessary to hold and protect them from degradation.
Numerous investigations have recently focused on micro- and nanostructured materials due to the drastic increase in the surface area-to-volume ratio compared with the bulk materials. One of the promising nano-structured materials is porous silicon (PSi), well known for photonic applications, sensors, and novel drug delivery methods [10–15]. Various applications of PSi in bio-nanotechnology are possible due to its advantageous properties namely tunable pore dimensions, large surface area, multilayered photonic structures, easy and cheap fabrication method, and biocompatibility. The exceptional electrical and optical properties and the particular multilayered photonic structures offer unique application possibilities in integrated optoelectronic and biosensing (biophotonic) devices as well [10, 13, 14]. On the other hand, meso- and macroporous silicon assures good conditions for the penetration of the required biomolecules. The pore size and optical properties are adjustable during the wet electrochemical etching process, which is used to fabricate the well-arranged one-dimensional photonic structure .
In this work, RC was immobilized on the surface of porous silicon microcavities via two different methods: covalent binding and non-covalent attachment via a specific peptide interface (‘peptide binding’). In both cases, the RC preserved its activity, but the efficiency of the two methods turned out to be clearly different.
Rhodobacter sphaeroides R-26 cells were grown photo-heterotrophically [17, 18]. RCs were prepared by LDAO (N N-dimethyldodecylamine-N-oxide; Fluka AG, St. Gallen, Switzerland) solubilization and purified by ammonium sulfate precipitation, followed by DEAE Sephacel (Sigma-Aldrich Corporation, St. Louis, MO, USA) anion-exchange chromatography.
Porous silicon microcavity (PSiMc) structures were prepared by wet electrochemical etching process using boron-doped p++ type silicon wafers (thickness 500 to 550 μm) with a 0.002 to 0.004 ohm·cm resistivity, and with a crystallographic orientation of (100). Silicon substrates were etched at room temperature with an electrolyte consisting of HF (48%), ethanol (99.9%), and glycerol (99.99%), in the volumetric ratio of 3:7:1. Current densities of 85 and 40 mA/cm2 were used to produce high and low porosity layers, respectively. As-etched PSi samples were thermally oxidized at 800°C .
Binding of RC protein within the PSiMc scaffold was performed via two different methods: (1) covalent binding through a three-step conjugation method with 3-aminopropyl-triethoxysilane (APTES) and glutaraldehyde (GTA) as cross-linker molecule  and (2) peptide functionalization. In the first method, silanization of the surface with APTES ensures free amine groups on the surface. Subsequent treatment by GTA, an amine-targeted homobifunctional cross-linker molecule, and finally by RC ensures covalent linkage between APTES and the protein. The second method (i.e., peptide functionalization) is based on the binding of RC to PSiMc by strong physical attachment through a hydrophobic peptide layer (SPGLSLVSHMQT). This peptide, elaborated via phage display technology, reveals a high and specific binding affinity for the p++ Si material .
Atomic force microscopy
Atomic force microscopy (AFM) images were recorded in air, with an Asylum MFP-3D head equipped with a Molecular Force Probe 3D controller (Asylum Research, Santa Barbara, CA, USA). Images were acquired in tapping mode using rectangular silicon cantilevers with a tip radius smaller than 10 nm; typically 7 nm (Olympus Micro Cantilever, OMCL-AC240TS, Olympus Corporation, Shinjiku, Tokyo, Japan). Images were taken at 1 Hz scan rate and digitized in 512 × 512 pixels.
Scanning electron microscopy
Scanning electron microscopy (SEM) was performed by a Hitachi S-4700 Type II FE-SEM (Hitachi High-Tech, Minato-ku, Tokyo, Japan) equipped with a cold field emission gun operating in the range of 5 to 15 kV. The samples were mounted on a conductive carbon tape and sputter-coated by a thin Au/Pd layer in Ar atmosphere prior to the measurement. Elemental analysis of the samples was performed using energy-dispersive X-ray spectroscopy (EDX) with a RÖNTEC XFlash Detector 3001 (Rontec Holdings AG, Berlin, Germany) coupled with a silicon drift detector.
Reflectivity spectra were recorded with a Bruker 66 V Fourier transform infrared spectrometer, using a Bruker A 510, 11° specular reflection unit (BRUKER AXS GMBH, Karlsruhe, Germany). The PSi samples were illuminated with the tungsten source, and the reflected beam was detected with the silicon diode detector. The resulting spectra were captured in the range of 25,000 to 9,000 cm−1 (400 to 1,100 nm) after each modification step of PSiMc structures. All spectra were averaged over 100 scans with a spectral resolution of 2 cm−1.
The PSiMc sample containing the bound RCs was placed in a 1 × 1-cm spectroscopic cuvette next to its rear wall facing the Xe flash beam. The cuvette was filled with buffer (10 mM TRIS, pH 8.0, 100 mM NaCl, 0.03% LDAO) containing reduced horse heart cytochrome c (Sigma-Aldrich) as electron donor and UQ-0 (2,3-methoxy-5-methyl-1,4-ubiquinone; Sigma-Aldrich) as electron acceptor for the light-activated RC. The oxidation of cytochrome c by RCs bound to PSi was checked by steady state absorption measurements using a UNICAM 4 spectrophotometer (Unicam Limited, Cambridge, UK). Cytochrome c was reduced by ascorbate before the experiments were conducted.
Results and discussion
Binding efficiency measurements
Other elements such as Au, Pd, and Al were also detected to some extent. The gold, palladium, and aluminum signals originate from the sample pre-preparation phase (sputtering and sample holders).
The binding of RC to PSiMc can be modeled by saturation characteristics so that it follows straight lines in a logarithmic representation. The saturation curve for the GTA method might indicate a slight biphasic character as compared with the strictly monophasic behavior with the peptide method. The slope of the fitted line is about two times larger (12.0 nm) for the peptide method compared with the one found for the initial phase (5.0 nm) and almost the same as the second phase (13.6 nm) of the GTA method. Hence, it can be concluded that the binding affinity of the RCs to the peptide-coated PSi is about twice as large as to the GTA.
The overall electron transfer through the RC in living organisms and in reconstituted systems is coupled to the oxidation of cytochrome c2 (the native electron donor) on the donor side and to the redox cycle of quinones on the acceptor side of the protein. Direct optical detection of cytochrome photooxidation in the cytochrome cycle is a reliable method of tracking the steps of the RC photocycle [22, 23]. The oxidation of cytochrome c can be followed by the change in the spectra, i.e., the gradual decrease in the absorption mainly at 550 nm after every flash excitation.
Cytochrome c oxidation shows that the RC photocycle could be restored after reconstitution of the donor and acceptor sites with cytochrome c and UQ, respectively. Hence, the specific binding sites of the PSi-bound protein stayed accessible for these externally added agents.
Successful infiltration of reaction center into PSiMc photonic structure and the retention of its photochemical activity were demonstrated. After reconstitution of the donor and acceptor sites, the RC photocycle was also restored, i.e., the accessibility of the secondary quinone site and of the cytochrome binding site was not blocked in the PSiMc matrix. This functional integrity is promising in terms of further research into the properties and applicability of this photo excitable semiconductor biophotonic material containing the photosynthetic reaction center, an exceptionally efficient natural light harvesting system.
This work was supported by grants from Hungarian Academy of Sciences (MTA) and the National Council for Science and Technology of Mexico (CONACYT), project no. 122017; the Swiss National Science Foundation (IZ73Z0_128037/1) and Swiss Contribution (SH/7/2/20); and the Hungarian-French Intergovernmental S&T Cooperation Program, project no. 10-1-2011-0735 (Hungary) and 25030RB (France) and the COST TD1102.
- Xua J, Bhattacharya P, Váró G: Monolithically integrated bacteriorhodopsin/semiconductor opto-electronic integrated circuit for a bio-photoreceiver. Biosens Bioelectron 2004, 19: 885–892. 10.1016/j.bios.2003.08.018View ArticleGoogle Scholar
- Meunier CF, Rooke JC, Hajdu K, Cutsem PV, Cambier P, Leonard A, Su BL: Insight into cellular response of plant cells confined within silica-based matrices. Langmuir 2010, 26: 6568–6575. 10.1021/la9039286View ArticleGoogle Scholar
- Shoseyov O, Levy I: Nanobiotechnology: Bioinspired Devices and Materials of the Future. Humana Press Inc., Totowa; 2008.View ArticleGoogle Scholar
- Ormos P, Fábián L, Oroszi L, Wolff EK, Ramsden JJ, Dér A: Protein-based integrated optical switching and modulation. Appl Phys Lett 2002, 80: 4060–4062. 10.1063/1.1481197View ArticleGoogle Scholar
- Hajdu K, Szabó T, Magyar M, Bencsik G, Németh Z, Nagy K, Forró L, Váró G, Hernádi K, Nagy L: Photosynthetic reaction center protein in nanostructures. Phys Status Solidi B 2011, 248: 2700–2703. 10.1002/pssb.201100046View ArticleGoogle Scholar
- Darder M, Aranda P, Ruiz-Hitzky E: Bionanocomposites: a new concept of ecological, bioinspired, and functional hybrid materials. Adv Mater 2007, 19: 1309–1319. 10.1002/adma.200602328View ArticleGoogle Scholar
- Allen JP, Williams JC: Photosynthetic reaction centers. FEBS Lett 1998, 438: 5–9. 10.1016/S0014-5793(98)01245-9View ArticleGoogle Scholar
- Nagy L, Hajdu K, Fisher B, Hernádi K, Nagy K, Vincze J: Photosynthetic reaction centres – from basic research to application – possibilities. Notulae Scientia Biologica 2010, 2: 07–13.Google Scholar
- Wraight CA, Clayton R: The absolute quantum efficiency of bacteriochlorofill photooxidation in reaction centres of Rhodopseudomonas sphaeroides. Biochem Biophys Acta 1974, 333: 246–260. 10.1016/0005-2728(74)90009-7Google Scholar
- Aroutiounian VM, Martirosyan KS, Hovhannisyan AS, Soukiassian PG: Use of porous silicon for double- and triple-layer antireflection coatings in silicon photovoltaic converters. Journal of Contemporary Physics (Armenian Academy of Sciences) 2008, 43: 72–76.Google Scholar
- Martin M, Palestino G, Cloitre T, Agarwal V, Zimanyi L, Gergely C: Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity. Appl Phys Lett 2009, 94: 223313. 10.1063/1.3148698View ArticleGoogle Scholar
- Thompson CM, Nieuwoudt M, Ruminski AM, Sailor MJ, Miskelly GM: Electrochemical preparation of pore wall modification gradients across thin porous silicon layers. Langmuir 2010, 26: 7598–7603. 10.1021/la904408hView ArticleGoogle Scholar
- Estephan E, Saab M-B, Agarwal V, Cuisinier FJG, Larroque C, Gergely C: Peptides for the biofunctionalization of silicon for use in optical sensing with porous silicon microcavities. Adv Funct Mater 2011, 21: 2003–2011. 10.1002/adfm.201002742View ArticleGoogle Scholar
- Palestino G, Legros R, Agarwal V, Pérez E, Gergely C: Functionalization of nanostructured porous silicon microcavities for glucose oxidase detection. Sensors and Actuators B 2008, 135: 27–34. 10.1016/j.snb.2008.07.013View ArticleGoogle Scholar
- Xiao L, Gu L, Howell SB, Sailor MJ: Porous silicon nanoparticle photosensitizers for singlet oxygen and their photo toxicity against cancer cells. ACS Nano 2011, 5: 3651–3659. 10.1021/nn1035262View ArticleGoogle Scholar
- Agarwal V, del Río JA: Tailoring the photonic band gap of a porous silicon dielectric mirror. Appl Phys Lett 2003, 82: 1512–1514. 10.1063/1.1559420View ArticleGoogle Scholar
- Ormerod JG, Ormerod KS, Gest H: Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys 1961, 94: 449–463. 10.1016/0003-9861(61)90073-XView ArticleGoogle Scholar
- Nagy L, Puskás Á, Tandori J, Droppa M, Horváth G: Effect of DCMU on photosynthetic purple bacteria. Photosynthetica 1991, 25: 167–171.Google Scholar
- Dorogi M, Bálint Z, Mikó C, Vileno B, Milas M, Hernádi K, Forró L, Váró G, Nagy L: Stabilization effect of single walled carbon nanotubes on the functioning of photosynthetic reaction centers. J Phys Chem B 2006, 110: 21473–21479. 10.1021/jp060828tView ArticleGoogle Scholar
- Oda I, Hirata K, Watanabe S, Shibata Y, Kajino T, Fukushima Y, Iwai S, Itoh S: Function of membrane protein in silica nanopores: incorporation of photosynthetic light-harvesting protein LH2 into FSM. J Phys Chem B 2006, 110: 1114–1120. 10.1021/jp0540860View ArticleGoogle Scholar
- Oda I, Iwaki M, Fujita D, Tsutsui Y, Ishizaka S, Dewa M, Nango M, Kajino T, Fukushima Y, Itoh S: Photosynthetic electron transfer from reaction center pigment-protein complex in silica nanopores. Langmuir 2010, 26: 13399–13406. 10.1021/la101810vView ArticleGoogle Scholar
- Kleinfeld D, Okaamura MY, Feher G: Electron transfer in reaction centers of Rhosopseudomonas sphaeroides. I. Determination of the charge recombination pathway of D+QAQB- and free energy and kinetic relations between QA-QB and QAQB-. Biochem Biophys Acta 1984, 766: 126–140. 10.1016/0005-2728(84)90224-XGoogle Scholar
- Osváth S, Maróti P: Coupling of cytochrome and quinone turnovers in the photocycle of reaction centers from the photosynthetic bacterium Rhodobacter sphaeroides. Biophys J 1996, 73: 972–982.View ArticleGoogle Scholar
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