Cytotoxicity and variant cellular internalization behavior of water-soluble sulfonated nanographene sheets in liver cancer cells
© Corr et al.; licensee Springer. 2013
Received: 2 January 2013
Accepted: 18 March 2013
Published: 2 May 2013
Highly exfoliated sulfonated graphene sheets (SGSs), an alternative to graphene oxide and graphene derivatives, were synthesized, characterized, and applied to liver cancer cells in vitro. Cytotoxicity profiles were obtained using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, WST-1[2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, and lactate dehydrogenase release colorimetric assays. These particles were found to be non-toxic across the concentration range of 0.1 to 10 μg/ml. Internalization of SGSs was also studied by means of optical and electron microscopy. Although not conclusive, high-resolution transmission and scanning electron microscopy revealed variant internalization behaviors where some of the SGS became folded and compartmentalized into tight bundles within cellular organelles. The ability for liver cancer cells to internalize, fold, and compartmentalize graphene structures is a phenomenon not previously documented for graphene cell biology and should be further investigated.
KeywordsCytotoxicity Sulfonated graphene sheets Cancer cells
Carbon-derived nanoparticles (NPs) such as single- and multi-walled carbon nanotubes, fullerenes, and graphene are all receiving attention because of their interesting and unusual electronic, thermal, and mechanical properties. We have recently demonstrated a facile route towards the synthesis of nanosized water-soluble sulfonated graphene sheets (SGSs) that use graphite as the starting material. This method relies on the addition of phenyl radicals with subsequent sulfonation of the phenyl groups and produces fewer defects and holes that can be introduced into the graphene plates through the use of heavy sonication. A possible application of these SGSs is within the medical sector due to their enhanced solubility (compared to other graphene derivatives) and potential for surface modifications for attachment of biomolecules and drugs. However, the interaction of SGSs with biological systems has yet to be investigated and is the basis of the work described herein.
To date, much of the biological work regarding graphene has focused on assessing the cytotoxicity, cell adhesion, proliferation, and antibacterial properties of graphene oxide (GO)[5–8] as well as biodistribution, toxicology, and internalization of various suspensions of GO complexes. These include 125I and 188Re radioisotope-labeled GO[9, 10], PEGylated GO for cellular imaging and delivery of water-insoluble cancer drugs[11–13], and the imaging and treatment of brain, lung, and breast xenograft tumors in mice through the use of photothermal light therapy from the absorption of near-infrared (NIR) light by PEGylated GO with fluorescent Cy7 probes.
Toxicity analysis (in vitro) of GO (prepared using chemical vapor deposition or the modified Hummers method) on lung[16, 17] and neuronal cell lines (A549 and PC12, respectively) has shown concentration-dependent cytotoxicity. The exact mechanism of cell death from GO remains uncertain although a slight increase in lactate dehydrogenase (LDH) from cells, generation of reactive oxygen species, and weak activation of a caspase-3-mediated apoptosis pathway have all been reported. These reports suggest GO cytotoxicity from either direct cellular membrane damage or activation of natural cellular suicide mechanisms.
Similarly, in vivo mouse toxicology studies have shown that GO nanoplatelets of diameters 10 to 700 nm apparently cause no acute toxicities at low doses[9, 10]. However, at high doses (10 mg/kg), significant pathological changes such as granulomatous lesions, pulmonary edema, inflammatory cell infiltration, and fibrosis were observed throughout the lungs.
In light of the potential applications of graphene materials in drug delivery, imaging, and thermal therapy, but with limitations due to cytotoxicity of GO, we sought to investigate the in vitro interaction of our highly water-soluble SGS with liver cancer cells. Our initial studies using the standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), WST-1[2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1), and LDH colorimetric assays have shown that SGSs are non-toxic up to concentrations of 10 μg/ml. We also show that liver cancer cell lines (SNU449 and Hep3B) can internalize SGSs of diameters up to 5 μm, which in some cases are comparable to the size of the cells themselves. Preliminary electron microscopy analysis also suggests that these cells are capable of folding and compartmentalizing sheets of smaller sizes (approximately 1.41 μm) although more work should be undertaken to validate.
Since graphene has been documented to be the hardest material known, this unique behavior of water-soluble SGS with cells is counterintuitive and suggests a novel finding that may have far-reaching applications in biology and medicine such as enhanced drug delivery (due to the large graphene surface area), and should warrant further investigation. Given that these SGSs are non-toxic up to 10 μg/ml, we feel they can be used as an adequate scaffold to simultaneously attach targeting moieties such as EGFR antibodies (e.g., cetuximab, C225) and chemo-agents such as doxorubicin and gemcitabine in a bid to treat hepatocellular carcinoma legions. The use of a targeted thermal ‘trigger’ such as photon activation (i.e., NIR light) or radiofrequency electric fields could allow these SGSs to release their cargo into the cells upon irradiation by a stimuli. Such a scheme has recently been reported using cisplatin-filled ultra-short carbon nanotubes that release their cargo upon exposure to high-intensity radiofrequency electric fields.
Sample preparation and characterization
Samples were obtained from Mukherjee et al.. In their technique, highly exfoliated SGSs can be synthesized by sulfonation of commercially available graphite (particle size < 20 μm) in oleum to overcome the cohesive van deer Waals attractions between adjacent sheets. Their exfoliation method was selected over the procedure by Si et al. as it produces fewer defects and holes that can be introduced into the graphene plates through the use of heavy sonication. In brief, the addition of benzoyl peroxide to a suspension of graphite in benzene at 75°C to 80°C provided phenylated graphite, the sulfonation of which by oleum leads to highly-exfoliated graphene sheets which can be further converted into a sodium salt by the addition of 1 M sodium hydroxide. This material, in powder form, is highly soluble in water (approximately 2.1 mg/ml) due to the p-sulphonated substituents, and it is relatively free of basal plane defects that typically result from the removal of the oxygen functionality of comparable GO compounds.
The SGSs in powder form were characterized via Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Raman spectra of the initial graphite material were compared to SGSs using a Renishaw 1000 micro-Raman system (Gloucestershire, UK) with a 514-nm excitation laser source. Multiple spectra were taken[3–5] and normalized to the G band. TGA data were taken using a model SDT 2960 TA (TA Instruments, Newcastle, DE, USA) instrument in both an argon and air atmosphere. Samples were first degassed at 80°C and then heated at 10°C/min to 700°C and held there for 20 min. This allowed for accurate percentage determination of the sodium sulfonate groups (approximately 6%). XPS data were obtained using a physical electronics (PHI QUENTERA, Chanhassen, MN, USA) XPS/ESCA system with a base pressure of 5 × 10−9 Torr. A monochromatic Al X-ray source at 100 W was used with a pass energy of 26 eV and a 45° takeoff angle. The beam diameter was 100.0 μm. Low- and high-resolution survey scans of the elements C, O, Na, and S were taken. At least two separate locations were analyzed for each sample. For AFM studies, aqueous solution of SGSs at 50 mg/l was drop-cast onto freshly cleaved mica and placed in a desiccator for 24 h prior to imaging. Tapping-mode AFM images were taken in air under ambient conditions on a Digital Instruments Nanoscope IIIA (Digital Instruments, Tonawanda, NY, USA).
Cell culture studies
SGS cytotoxicity was investigated using multiple assays. Cell membrane integrity was evaluated using a LDH release assay. Cell proliferation/metabolic activity was investigated using the popular MTT and WST-1 colorimetric assays. For in vitro experiments, approximately 3 mg of the SGS powder was added to 3 ml of phosphate-buffered saline (PBS) to create two suspensions of concentration 1,000 μg/ml. All samples were sterilized for 20 min using a bench-top UV sterilizer. SNU449 and Hep3B liver cancer cells were utilized for the experiments (American Type Culture Collection, Bethesda, MD, USA). The cells were maintained in standard culture conditions with 10% fetal calf serum and penicillin/streptomycin at 37°C. Cell morphology was analyzed using real-time bright-field optical imaging.
SNU449 and Hep3B cells were plated in 96-well plates at a density between 1,000 to 2,000 cells per well. After 24 h, the SNU449 and Hep3B cells were exposed to increasing concentrations (0.1, 1.0, 10, and 100 μg/ml) of SGSs in PBS and were compared to a PBS only control group (all suspensions were lightly sonicated for 5 min before use). Cell viability was assessed at 24, 72, and 120 h after exposure to the SGSs. At each time point, the media (100 μl) was carefully aspirated and replaced before adding MTT reagent to each well and incubating for 4 h. The media was again carefully removed, and purple formazan crystals were dissolved in dimethyl sulfoxide (DMSO). The 96-well plates were then spun down at 3,500 rpm for 5 min (to force any cells/SGS debris to the bottom of the well) where 50 μl of the colored media was withdrawn and placed into a fresh 96-well plate. Absorbance was interpreted at 570 nm for each well using a SPECTROstar Nano plate reader (BMG Labtech Inc., Cary, NC, USA).
These studies were prepared similar to the MTT assay but for a shorter duration (24, 48, and 72 h) as MTT assays showed that maximum toxicity occurred at 72 h. Also, it was harder to keep the control cells from overgrowing for times greater than 72 h. At each time point, WST-1 reagent was added to each well and incubated for 3 h. The 96-well plate was then spun down at 3,500 rpm for 5 min (to force any cells/SGS debris to the bottom of the well) where 50 μl of the colored media was withdrawn and placed into a fresh 96-well plate. This negated any effects from inherent SGS absorption as all the SGSs were contained at the bottom of the discarded well. Absorbance was interpreted at 450 nm for each well using a SPECTROstar Nano plate reader (BMG Labtech Inc.).
SNU449 and HEP3B cells were exposed to various concentrations of SGSs (0.1, 1.0, 10.0, and 100 μg/ml) for 24, 48, and 72 h, and the cell-free supernatant was removed. Maximum LDH release was obtained by exposing the cells to a 2% Triton-X 100 solution to permeabilize the membranes. LDH activity was determined by the use of a cytotoxicity detection kit purchased from Roche Applied Science (Indianapolis, IN, USA). Aliquots of the cell culture media from the SGS-exposed samples, untreated samples, and the permeabilized samples were added to a 96-well plate, and an equal volume of LDH cytotoxicity detection reagent was added. The 96-well plates were read on a spectrophotometer, and the absorbance at 492 nm was measured. Calculations were performed as per the recommendations of the kit. To show that SGS does not interfere with the kit, cells were permeabilized with a 2% Triton-X 100 solution. The lysate was incubated with various concentrations of SGS for 24 h. No difference was observed for any of the control samples indicating that SGSs do not interfere with the assay.
Viability was measured with flow cytometry (LSRII, BD Biosciences, Franklin, NJ, USA) as described previously. Briefly, cell media was aspirated, and the adherent cells were collected after trypsinization. Each sample was washed and stained with annexin V-FITC and propidium iodide (PI) without fixation or permeabilization. Annexin V is a protein that binds to phosphatidylserine, which is externalized in apoptotic cells. Propidium iodide fluoresces when it is bound to DNA in membrane-damaged cells. Cells that were negative for both markers were characterized as viable. Approximately 50,000 events were measured for each sample. Due to sample availability, only one time point (24 h) was measured on one cell line (SNU449) at two concentrations (10 and 100 μg/ml). As such, these data have been placed in the Additional file1.
Real-time optical bright-field microscopy
Hep3B cells were cultured in glass bottom (no. 1.5) 24-well plates purchased from MatTek Corporation (Ashland, MA, USA). After overnight incubation, the cells formed non-confluent monolayers. The 24-well plate was placed in an incubator enclosing a 1X81 Olympus microscope (Center Valley, PA, USA) equipped with a DSU Confocal Attachment and a ×60 oil immersion objective. The cells were allowed to equilibrate with the incubator environment (37°C, 5% CO2) before adding pre-warmed SGSs and acquiring images. Eight Z-plane images were acquired with a gap of 1 μm every 15 min. A typical experiment comprised of 10 to 15 waypoints. In-focus light from all planes was merged and is represented in the still shots and the movies. Hep3B cells with no exposure to SGS were also imaged as a control.
Transmission/scanning electron microscopy
For transmission electron microscopy (TEM) imaging, 25,000 Hep3B or SNU449 cells were plated in 12-well plates. After 24 h, the cells were exposed to the SGS at 10 μg/ml for 24 h. The media was removed, and cells were washed twice with PBS. The cells were then harvested after trypsinization and washed once more with PBS. Finally, the cells were resuspended in Trump’s Fixative (BBC Biochemical, Seattle, WA, USA). Samples were washed with 0.1% cacodylate-buffered tannic acid, treated with 1% buffered osmium tetroxide, and stained with 1% uranyl acetate. The samples were ethanol dehydrated and embedded in LX-112 medium. After polymerization, the samples were cut with an UltraCut E Microtome (Leica, IL, USA), double stained with uranyl acetate/lead citrate in a Leica EM stainer, and imaged with a JEM 1010 TEM (Jeol USA, Inc., Peabody, MA, USA) at an accelerating voltage of 80 kV. Images were acquired with an AMT Imaging System (Advanced Microscopy Techniques Corp., Woburn, MA, USA). For SEM, the cells were prepared in a similar manner. The dried samples were coated with a 35-nm-thick platinum layer. Samples were imaged using a JSM 5900 scanning electron microscope (JEOL USA, Inc.) equipped with a backscatter electron detector and digital camera. The beam energy was 5 kV.
Results and discussion
Cytotoxicity profiles of SGSs
Previous work by Zhang et al. demonstrated a similar MTT concentration-dependent viability profile with neural phaeochromocytoma-derived PC12 cells exposed to graphene synthesized via CVD (purified using a diluted hydrochloric acid wash with sonication). They showed cell viability of approximately 40% after 24 h of exposure to their graphene particles at a concentration of 100 μg/ml, which is similar to MTT values seen in this work. In comparison, Chang et al. also demonstrated a concentration-dependent profile which was however not time dependent since they observed similar viability profiles at 24, 48, and 72 h.
Although the MTT and WST-1 profiles are generally identical for time periods 24 to 72 h (with possibly the exception of the WST-1 results which show a weak time-dependent and concentration-dependent response), the major difference is the drastic loss in viability for concentrations of 100 μg/ml observed in the MTT assay. This observation could be explained by interactions of SGSs with insoluble MTT formazan crystals (formed after the enzymatic reduction of MTT within the cells) which stabilize their structure and prevent them from becoming solubilized by DMSO. This has already been observed by Wörle-Knirsch et al.. In their work, they showed that single-walled carbon nanotubes (SWNTs) were found to be non-toxic when using assays such as LDH, annexin V, and PI staining, mitochondrial membrane potential, as well as other tetrazolium salt-based water-soluble assays such as WST-1, XTT, or INT. However, the MTT assay was the only assay which displayed SWNT cytotoxicity.
Propidium iodide is a cell impermeable fluorophore that can bind to the DNA of cells which have lost nuclear and plasma membrane integrity. From our fluorescence-activated cell sorting (FACS) analysis shown in Additional file1: Figure S5, we found that with an increasing concentration of SGS nanoparticles, the intensity of positive PI-stained cells increased from approximately 1.9% to 10.3%, suggesting slight cell membrane structural damage, while the majority of cells remain healthy and viable at approximately 93% ± 2.4%. Phosphatidylserine (PS) externalization is an early event in the apoptosis cascade. Annexin V binds to PS with high affinity. Our FACS analysis hence also demonstrates that very few cells were annexin V positive 24 h after exposure to SGS which ruled out apoptosis as a significant cell death mechanism, as was similarly reported for GO materials[16, 18].
Cellular internalization of SGSs
In Figure 4D, it appears as if the Hep3B cell is actively internalizing multiple, stacked SGS of height approximately 35 nm, but is most likely a single SGS which looks thicker due to the platinum layer. The folding phenomenon is also evident in Figure 4E where folding of SGS can be seen in the bottom left corner and bottom midsection of the image, as indicated by the white arrows. There is also evidence of slightly deformed SGS on top of the cellular surface in the upper right-hand section. Finally, Figure 4F depicts the images of both SGS deformation and internalization of large pieces of graphitic materials. The appearance of pseudopodia over the surface of the SGS is indicated by the red arrows.
Figure 5D,F shows close up images of two areas of Figure 5E to reveal a stained black circular particle (Figure 5E) and a more transparent, slightly smaller, circular particle (Figure 5F). As these particles are of the same diameter as the SGS previously characterized, they are likely SGS that have internalized into the cell without folding or compartmentalization. As previously indicated, the large difference in contrast between these two SGS structures could be due to uranyl ions binding to the functionalized SGS or due to multiple stacked graphene layers.
Given that graphene is thought to be the hardest material known, it is counterintuitive to believe that liver carcinoma cells are capable of folding and compartmentalizing graphene sheets. However, if these sheets contained structural defects such as point defects, single vacancies, multiple vacancies, carbon adatoms, dislocation-like defects, or edge defects, as extensively reviewed by Banhart et al., the cells may be able to fold the sheets, one at a time, along these defect lines (in a ‘shedding nature’) and compartmentalize them within phagosomes or vesicles using reasonably low-energy processes. The defect content of the SGS, in relation to the starting graphite material, can be indicated by the relative intensity of the Raman D band to G band ratio, located at approximately 1,350 and 1,580 cm−1, respectively. Although the synthesis procedure and Raman characterization shown in Additional file1: Figure S2 shows a weak D band enhancement after exfoliation due to functionalization of the graphitic edges, it remains unclear as to what defects, if any, are inherent to the graphene nanoplatelets.
We have investigated the cytotoxicity and internalization of highly exfoliated, water-soluble SGSs when exposed in vitro to highly aggressive human liver cancer cells (SNU449 and Hep3B). Both MTT and WST-1 colorimetric assays displayed a similar concentration- and time-dependent cytotoxicity profile for concentrations of 0.1 to 10 μg/ml. These trends were also evident from LDH observations. However, the SGSs seemed to be toxic to both cell lines at the highest concentration of 100 μg/ml. We have also observed an interesting cellular internalization phenomenon for graphene materials for the first time. The cancer cells were capable of internalizing relatively large SGSs with diameters comparable to the cells themselves as well as smaller SGS having heights indicative of single graphene sheets. Although not conclusive, there is evidence to suggest that due to graphene structural defects, the cancer cells are also able to actively fold and compartmentalize these sheets. We speculate that the findings reported here may encourage the development of SGSs for applications in drug delivery, medical imaging, and even hyperthermic cancer therapy by NIR and/or radio frequency heating. To date, such applications have been explored for more rigid carbon nanostructures such as fullerenes and nanotubes[29–32], but a non-toxic, more flexible (foldable), and larger surface-area material as provided by graphene offers an alternative design strategy.
Atomic force microscopy
Fluorescence-activated cell sorting
Phosphatidylserine, SGSs, sulphonated graphene sheets
Transmission electron microscopy
X-ray photoelectron spectroscopy
This work was funded by the NIH (U54CA143837), the NIH M.D. Anderson Cancer Center Support Grants (CA016672), the V Foundation (SAC), The Welch Foundation (C-0627, LJW; C-0490, WEB), and an unrestricted research grant from the Kanzius Research Foundation (SAC, Erie, PA, USA). We thank Kristine Ash from the Department of Surgical Oncology, M.D. Anderson Cancer Center for the administrative assistance, Kenneth Dunner, Jr. of The High Resolution Electron Microscopy Facility at The University of Texas M.D. Anderson Cancer Center (NCI Core grant CA16672) for providing TEM imaging services, and Jared Burks of the Cytometry and Cellular Imaging Core Facility (NIH MDACC support grant CA016672) for providing invaluable assistance with real-time optical imaging.
- Geim AK, Novoselov KS: The rise of graphene. Nature Materials 2007, 6(3):183–191. 10.1038/nmat1849View ArticleGoogle Scholar
- Balandin AA, Ghosh S, Bao W, Calizo I, Teweldebrhan D, Miao F, Lau CN: Superior thermal conductivity of single-layer graphene. Nano Lett 2008, 8(3):902–907. 10.1021/nl0731872View ArticleGoogle Scholar
- Lee C, Wei X, Kysar JW, Hone J: Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science 2008, 321(5887):385–388. 10.1126/science.1157996View ArticleGoogle Scholar
- Mukherjee A, Kang J, Kuznetsov O, Sun YQ, Thaner R, Bratt AS, Lomeda JR, Kelly KF, Billups WE: Water-soluble graphite nanoplatelets formed by oleum exfoliation of graphite. Chem Mater 2011, 23(1):9–13. 10.1021/cm100808fView ArticleGoogle Scholar
- Kalbacova M, Broz A, Kong J, Kalbac M: Graphene substrates promote adherence of human osteoblasts and mesenchymal stromal cells. Carbon 2010, 48(15):4323–4329. 10.1016/j.carbon.2010.07.045View ArticleGoogle Scholar
- Chen H, Muller MB, Gilmore KJ, Wallace GG, Li D: Mechanically strong, electrically conductive, and biocompatible graphene paper. Adv Mater 2008, 20(18):3557–3561. 10.1002/adma.200800757View ArticleGoogle Scholar
- Hu W, Peng C, Luo W, Lv M, Li X, Li D, Huang Q, Fan C: Graphene-based antibacterial paper. ACS Nano 2010, 4(7):4317–4323. 10.1021/nn101097vView ArticleGoogle Scholar
- Ryoo SR, Kim YK, Kim MH, Min DH: Behaviors of NIH-3T3 fibroblasts on graphene/carbon nanotubes: proliferation, focal adhesion, and gene transfection studies. ACS Nano 2010, 4(11):6587–6598. 10.1021/nn1018279View ArticleGoogle Scholar
- Yang K, Wan JM, Zhang SA, Zhang YJ, Lee ST, Liu ZA: In vivo pharmacokinetics, long-term biodistribution, and toxicology of PEGylated graphene in mice. ACS Nano 2011, 5(1):516–522. 10.1021/nn1024303View ArticleGoogle Scholar
- Zhang XY, Yin JL, Peng C, Hu WQ, Zhu ZY, Li WX, Fan C, Huang Q: Distribution and biocompatibility studies of graphene oxide in mice after intravenous administration. Carbon 2011, 49(3):986–995. 10.1016/j.carbon.2010.11.005View ArticleGoogle Scholar
- Liu ZR JT, Sun X, Dai H: PEGylated nano-graphene oxide for delivery of water-insoluble cancer drugs. J Am Chem Soc 2008, 10876–10877.Google Scholar
- Sun X, Liu Z, Welsher K, Robinson JT, Goodwin A, Zaric S, Dai H: Nano-graphene oxide for cellular imaging and drug delivery. Nano research 2008, 1(3):203–212. 10.1007/s12274-008-8021-8View ArticleGoogle Scholar
- Zhang LM, Xia JG, Zhao QH, Liu LW, Zhang ZJ: Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. Small 2010, 6(4):537–544. 10.1002/smll.200901680View ArticleGoogle Scholar
- Yang K, Zhang SA, Zhang GX, Sun XM, Lee ST, Liu ZA: Graphene in mice: ultrahigh in vivo tumor uptake and efficient photothermal therapy. Nano Lett 2010, 10(9):3318–3323. 10.1021/nl100996uView ArticleGoogle Scholar
- Hummers WS, Offeman RE: Preparation of graphitic oxide. J Am Chem Soc 1958, 80(6):1339. 10.1021/ja01539a017View ArticleGoogle Scholar
- Chang YL, Yang ST, Liu JH, Dong E, Wang YW, Cao AN, Liu Y, Wang H: In vitro toxicity evaluation of graphene oxide on A549 cells. Toxicol Lett 2011, 200(3):201–210. 10.1016/j.toxlet.2010.11.016View ArticleGoogle Scholar
- Hu WB, Peng C, Lv M, Li XM, Zhang YJ, Chen N, Fan C, Huang Q: Protein corona-mediated mitigation of cytotoxicity of graphene oxide. ACS Nano 2011, 5(5):3693–3700. 10.1021/nn200021jView ArticleGoogle Scholar
- Zhang YB, Ali SF, Dervishi E, Xu Y, Li ZR, Casciano D, Biris AS: Cytotoxicity effects of graphene and single-wall carbon nanotubes in neural phaeochromocytoma-derived PC12 cells. ACS Nano 2010, 4(6):3181–3186. 10.1021/nn1007176View ArticleGoogle Scholar
- Raoof M, Cisneros BT, Guven A, Phounsavath S, Corr SJ, Wilson LJ, Curley SA: Remotely triggered cisplatin release from carbon nanocapsules by radiofrequency fields. Biomaterials 2013, 34(7):1862–1869. 10.1016/j.biomaterials.2012.11.033View ArticleGoogle Scholar
- Si Y, Samulski ET: Synthesis of water soluble graphene. Nano Lett 2008, 8(6):1679–1682. 10.1021/nl080604hView ArticleGoogle Scholar
- Raoof M, Corr SJ, Kaluarachchi WD, Massey KL, Briggs K, Zhu C, Cheney MA, Wilson LJ, Curley SA: Stability of antibody-conjugated gold nanoparticles in the endolysosomal nanoenvironment: implications for noninvasive radiofrequency-based cancer therapy. Nanomedicine 2012, 8(7):1096–1105. 10.1016/j.nano.2012.02.001View ArticleGoogle Scholar
- Becerril HA, Mao J, Liu Z, Stoltenberg RM, Bao Z, Chen Y: Evaluation of solution-processed reduced graphene oxide films as transparent conductors. ACS Nano 2008, 2(3):463–470. 10.1021/nn700375nView ArticleGoogle Scholar
- Gomez-Navarro C, Weitz RT, Bittner AM, Scolari M, Mews A, Burghard M, Kern K: Electronic transport properties of individual chemically reduced graphene oxide sheets. Nano Lett 2007, 7(11):3499–3503. 10.1021/nl072090cView ArticleGoogle Scholar
- Wörle-Knirsch JM, Pulskamp K, Krug HF: Oops they did it again! Carbon nanotubes hoax scientists in viability assays. Nano Lett 2006, 6(6):1261–1268. 10.1021/nl060177cView ArticleGoogle Scholar
- Gass MH, Bangert U, Bleloch AL, Wang P, Nair RR, Geim AK: Free-standing graphene at atomic resolution. Nat Nanotechnol 2008, 3(11):676–681. 10.1038/nnano.2008.280View ArticleGoogle Scholar
- Banhart F, Kotakoski J, Krasheninnikov AV: Structural defects in graphene. ACS Nano 2011, 5(1):26–41. 10.1021/nn102598mView ArticleGoogle Scholar
- Lotya M, King PJ, Khan U, De S, Coleman JN: High-concentration surfactant-stabilized graphene dispersions. ACS Nano 2010, 4(6):3155–3162. 10.1021/nn1005304View ArticleGoogle Scholar
- Krishna V, Stevens N, Koopman B, Moudgil B: Optical heating and rapid transformation of functionalized fullerenes. Nat Nanotechnol 2010, 5(5):330–334. 10.1038/nnano.2010.35View ArticleGoogle Scholar
- Ajayan PM, Terrones M, de la Guardia A, Huc V, Grobert N, Wei BQ, Lezec H, Ramanath G, Ebbesen TW: Nanotubes in a flash—ignition and reconstruction. Science 2002, 296(5568):705. 10.1126/science.296.5568.705View ArticleGoogle Scholar
- Choi JH, Nguyen FT, Barone PW, Heller DA, Moll AE, Patel D, Boppart SA, Strano MS: Multimodal biomedical imaging with asymmetric single-walled carbon nanotube/iron oxide nanoparticle complexes. Nano Lett 2007, 7(4):861–867. 10.1021/nl062306vView ArticleGoogle Scholar
- Liang F, Chen B: A review on biomedical applications of single-walled carbon nanotubes. Curr Med Chem 2010, 17(1):10–24. 10.2174/092986710789957742View ArticleGoogle Scholar
- Gannon CJ, Cherukuri P, Yakobson BI, Cognet L, Kanzius JS, Kittrell C, Weisman RB, Pasquali M, Schmidt HK, Smalley RE, Curley SA: Carbon nanotube-enhanced thermal destruction of cancer cells in a noninvasive radiofrequency field. Cancer 2007, 110(12):2654–2665. 10.1002/cncr.23155View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.