Staphylococcus aureus was recognized as a major pathogen soon after its discovery in the late nineteenth century. This organism causes a broad range of conditions, ranging from asymptomatic colonization to severe invasive infections which can progress to complicated septicemia, osteomyelitis, septic arthritis, or endocarditis [1, 2]. S. aureus is a major cause of nosocomial infections and is responsible for significant morbidity, mortality, and an extended hospital stay [3, 4]. This Gram-positive bacterium possesses specific surface proteins such as fibronectin-binding proteins, collagen-binding proteins, and fibrinogen-binding proteins, which have been implicated as mediators in specific bacterial binding to the extracellular matrix and subsequent biofilm development [1, 5–7].
The increased use of prosthetic devices during the past decades has been accompanied by a constantly increased number of prosthetic device infections . S. aureus is a widespread bacterium, being found on the skin and mucosa of healthy persons; therefore, prosthesis-associated infections incriminating this pathogen are frequently encountered . Prosthesis-associated infections could be the results of microbial colonization by three routes: (a) direct inoculation at the time of implantation, (b) hematogenous spreading during bacteremia, or (c) direct contiguous spreading from an adjacent infectious focus .
One of the most severe complications is a biofilm-associated infection of a prosthetic device due to the fact that biofilm bacteria are different from planktonic cells, being usually more resistant. The biofilm cells are resistant to all kinds of antimicrobial substances: antibiotics, antiseptics, disinfectants; this kind of resistance, consecutive to biofilm formation, is phenotypic, behavioral, and more recently, called tolerance [43, 44].
Among the promising approaches to combat biofilm infections is the generation of surface modification of devices to reduce microbial attachment and biofilm development as well as incorporation of antimicrobial agents to prevent colonization. Essential oils (EOs) and their components are gaining increasing interest in the food, cosmetic, and pharmaceutical industries because of their relatively safe status, their wide acceptance by consumers, and their exploitation for potential multi-purpose functional use .
Plants of the genus Mentha produce a class of natural products known as mono-terpenes (C10), characterized by p-menthone skeleton. Members of this genus are the only sources for the production of one of the most economically important essential oil, menthol, throughout the world . Mentha piperita, commonly called peppermint, is a well-known herbal remedy used for a variety of symptoms and diseases, recognized for its carminative, stimulating, antispasmodic, antiseptic, antibacterial, and antifungal activities [4, 13, 14]. However, their use for clinical purposes is limited by the high volatility of the major compounds.
Due to their high biocompatibility  and superparamagnetic behavior, magnetite nanoparticles (Fe3O4) have attracted attention to their potential applications especially in biomedical fields [16, 17], such as magnetic resonance imaging [18–20], hyperthermia , biomedical separation and purification , bone cancer treatment , inhibition of biofilm development [23, 24], stabilization of volatile organic compounds , antitumoral treatment without application of any alternating magnetic field , drug delivery or targeting [27–33], modular microfluidic system for magnetic-responsive controlled drug release, and cell culture .This paper reports a new nano-modified prosthetic device surface with anti-pathogenic properties based on magnetite nanoparticles and M. piperita essential oil.