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A solid-phase dot assay using silica/gold nanoshells

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Abstract

We report on the first application of silica-gold nanoshells to a solid-phase dot immunoassay. The assay principle is based on staining of a drop (1 µl) analyte on a nitrocellulose membrane strip by using silica/gold nanoshells conjugated with biospecific probing molecules. Experimental example is human IgG (hIgG, target molecules) and protein A (probing molecules). For usual 15-nm colloidal gold conjugates, the minimal detectable amount of hIgG is about 4 ng. By contrast, for nanoshell conjugates (silica core diameter of 70 nm and gold outer diameter of 100 nm) we have found significant increase in detection sensitivity and the minimal detectable amount of hIgG is about 0.5 ng. This finding is explained by the difference in the monolayer particle extinction.

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Acknowledgments

This research was partially supported by grants from RFBR (Nos.05-02-16776, 04-04-48224), the targeted program “Research of cooperative and non-linear phenomena in light transport through mesascopic media as applied to development of diagnostical techniques in biology, medicine and industry” (No. RNP.2.1.1.4473). BK was supported by grants from the President of Russian Federation (MK 961.2005.2), CRDF (BRHE Annex BF4M06 Y2-B-06-08), and INTAS Young Scientist Fellowship Grant 06-1000014-6421. VZ was supported by grants from the National Institute of Biomedical Imaging and Bioengineering (NIH/NIBIB, nos. EB000873 and EB0005123).

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Correspondence to Nikolai Khlebtsov.

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Keywords

  • Colloidal gold
  • Silica/gold nanoshells
  • Solid-phase immunoassay