Table 3 Represents the bactericidal activity inE. coli DH5 α by agar well diffusion assay for free antibiotics and their respective Gnps conjugated form after different temperature and time stresses
From: In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics
Agents | Zone of inhibition forE. coli DH5 α strain in sq. cm | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Ampicillin | Streptomycin | Kanamycin | ||||||||
Free | Gnps conjugated | % Change | Free | Gnps conjugated | % Change | Free | Gnps conjugated | % Change | ||
Incubated for 10 min at | 26 °C | 3.085 | 3.569 | +15.688 | 2.189 | 2.453 | +12.060 | 3.371 | 4.545 | +34.826 |
50 °C | 2.806 | 6.141 | +118.85 | 1.378 | 1.622 | +17.707 | 2.063 | 3.663 | +77.557 | |
75 °C | 2.198 | 6.635 | +201.87 | 1.116 | 1.411 | +26.434 | 1.834 | 3.389 | +84.787 | |
90 °C | 2.107 | 6.707 | +218.32 | 0.053 | 1.324 | +2398.1 | 1.491 | 3.263 | +118.85 | |
Storage at room temp. (25–28°C) for | 0 day | 3.085 | 3.569 | +15.688 | 2.189 | 2.453 | +12.060 | 3.371 | 4.545 | +34.826 |
3 days | 2.929 | 3.142 | +7.272 | 2.126 | 2.361 | +11.054 | 2.283 | 4.199 | +83.925 | |
7 days | 2.646 | 2.823 | +6.689 | 1.486 | 2.049 | +37.887 | 1.562 | 4.024 | +157.62 | |
14 days | 2.561 | 2.593 | +1.250 | 1.055 | 1.483 | +40.569 | 1.501 | 3.879 | +158.43 | |
21 days | 2.540 | 1.941 | −23.583 | 0.913 | 1.345 | +47.317 | 1.338 | 3.645 | +172.42 | |
28 days | 1.965 | 1.209 | −38.473 | 0.547 | 0.945 | +72.761 | 1.239 | 3.459 | +179.18 | |