Effect of pH on the Interaction of Gold Nanoparticles with DNA and Application in the Detection of Human p53 Gene Mutation

Table 1 Target oligonucleotide sequences

ssDNA	No.	Sequence (5′-3′)
175wd		GGCACCCGCGTCCGCGCCATGGCCATCTAC
175G–A	1	GGCACCCGCGTCCCGCCATGGCCATCTAC
245wd		TGCATGGGCGGCATGAACCGGAGGCCCATC
245G–T	2	TGCATGGGCGCATGAACCGGAGGCCCATC
245G–A	3	TGCATGGGCGCATGAACCGGAGGCCCATC
248wd		GCATGAACCGGAGGCCCATCCTCACCATCA
248G–A	4	GCATGAACCGAGGCCCATCCTCACCATCA
248G–T	5	GCATGAACCGAGGCCCATCCTCACCATCA
248C–T	6	GCATGAACGGAGGCCCATCCTCACCATCA
249wd		GCATGAACCGGAGGCCCATCCTCACCATCA
249G1–T	7	GCATGAACCGGAGCCCATCCTCACCATCA
249G2–T	8	GCATGAACCGGAGCCCATCCTCACCATCA
249G-C	9	GCATGAACCGGAGCCCATCCTCACCATCA
273wd		GAACAGCTTTGAGGTGCGTGTTTGTGCCTG
273G–T	10	GAACAGCTTTGAGGTGCTGTTTGTGCCTG
273G–A	11	GAACAGCTTTGAGGTGCTGTTTGTGCCTG
282wd		TCCTGGGAGAGACCGGCGCACAGAGGAAGA
282C–T	12	TCCTGGGAGAGACGGCGCACAGAGGAAGA

The underline corresponds to the single base mismatch comparing with the wildtype (wd). 12 of the most frequent point mutations in exon 5, 7, and 8 of human p53 gene are included. 248wd and 249wd are the same sequences. The mutant sequences are numbered from 1 to 12