Figure 5
From: Regulation of Transgene Expression in Tumor Cells by Exploiting Endogenous Intracellular Signals
GFP expression after polymer/DNA complex was microinjected into HepG2 cells. Naked DNA, polymer only, and polymer/DNA complexes (C/A = 0.5, 1.0, and 2.0) were microinjected into HepG2 cells and GFP expression was detected 24 h after injection. PPC(S) contained a Ser phosphorylation site for PKCα, but the phosphorylation site was changed to Ala in PPC(A), leading to no phosphorylation by PKCα. Ro31-7549, a PKCα inhibitor