Figure 5

Drawing and transmission electron microscopy (TEM) image (a) and Prussian blue-positive cells (b) showing nanoparticles inside the cell (arrow). c: T2-weighted image of a rat spinal cord injected with nanoparticle-labeled MSCs. Arrowheads mark the injection sites, arrow the lesion populated with cells; Implanted nanoparticles labeled mouse ESCs were labeled with SPIO (e g). Cells were grafted intravenously. In vivo MRI was used to track their fate (h). Prussian blue staining confirmed the presence of iron oxide nanoparticles inside the cells (f). After 4 weeks post-implantation, grafted cells migrated to the lesion site and formed teratomas composed of tissue of all three germ layers (l m); In vitro differentiation of quantum dots labeling of hESCs (d) into neurons (i), hematopoietic cells (j) and endothelia cells (k) [44]