Table 3 Effects of nano-TiO2on the amplification of cytokine mRNA of mouse by real-time PCR analysis after intraperitoneal injection with nano-anatase TiO2suspensions for consecutive 14 days
From: The Acute Liver Injury in Mice Caused by Nano-Anatase TiO2
Cytokine | Nano-anatase (mg/kg BW) | |||||
---|---|---|---|---|---|---|
0 | 50 | 100 | 150 | 150-bulk | ||
Refer-actin | Ct | 21.035 | 19.93 | 21.4249 | 21.8257 | 21.038 |
Copies | 2.68E+07 | 5.64E+07 | 2.06E+07 | 1.57E+07 | 2.68E+07 | |
nf-κb | Ct | 23.144 | 21.7009 | 22.4526 | 22.2415 | 23.0754 |
Relative copies | 6.47E+06 | 1.71E+07 | 1.02E+07 | 1.19E+07 | 6.78E+06 | |
Ratio ofnf-κb/actin | 0.242 ± 0.012 | 0.303 ± 0.015* | 0.497 ± 0.025** | 0.756 ± 0.038** | 0.253 ± 0.013 | |
mif | Ct | 22.4026 | 21.7153 | 21.5595 | 21.5625 | 20.5766 |
Relative copies | 1.07E+07 | 1.70E+07 | 1.88E+07 | 1.88E+07 | 3.65E+07 | |
Ratio ofmif/actin | 0.398 ± 0.020 | 0.634 ± 0.032** | 0.913 ± 0.046** | 1.194 ± 0.060** | 0.647 ± 0.032** | |
il-1β | Ct | 24.2684 | 22.911 | 20.9252 | 22.2075 | 24.0418 |
Relative copies | 3.04E+06 | 7.57E+06 | 2.89E+07 | 1.22E+07 | 3.54E+06 | |
Ratio ofil-1β/actin | 0.113 ± 0.006 | 0.481 ± 0.024** | 0.511 ± 0.026** | 0.590 ± 0.030** | 0.132 ± 0.007* | |
il-6 | Ct | 25.076 | 22.6692 | 23.3343 | 22.8735 | 23.4873 |
Relative copies | 1.76E+06 | 8.9lE+06 | 5.70E+06 | 7.77E+06 | 5.14E+06 | |
Ratio ofil-6/actin | 0.066 ± 0.003 | 0.158 ± 0.008** | 0.276 ± 0.014** | 0.494 ± 0.025** | 0.192 ± 0.010** | |
crp | Ct | 23.9738 | 21.7188 | 23.152 | 22.907 | 23.0768 |
Relative copies | 3.70E+05 | 1.69E+07 | 6.44E+06 | 7.59E+06 | 6.77E+06 | |
Ratio ofcrp/actin | 0.138 ± 0.007 | 0.300 ± 0.015** | 0.312 ± 0.016** | 0.482 ± 0.024** | 0.253 ± 0.013** | |
tnf | Ct | 28.674 | 28.8403 | 28.659 | 28.3492 | 28.7297 |
Relative copies | 1.56E+05 | 1.39E+05 | 1.58E+05 | 1.94E+05 | 1.50E+05 | |
Ratio oftnf/actin | 0.028 ± 0.001 | 0.052 ± 0.003** | 0.076 ± 0.004** | 0.123 ± 0.006** | 0.056 ± 0.003 | |
il-4 | Ct | 23.7878 | 22.2796 | 21.1993 | 21.3352 | 22.906 |
Relative copies | 4.20E+06 | 1.16E+07 | 2.40E+07 | 2.19E+07 | 7.60E+06 | |
Ratio ofil-4/actin | 0.157 ± 0.008 | 0.205 ± 0.010** | 0.895 ± 0.045** | 1.392 ± 0.070** | 0.369 ± 0.018** | |
π-10 | Ct | 28.5199 | 27.022 | 28.6367 | 28.1555 | 28.169 |
Relative copies | 173E+05 | 4.75E+05 | 1.60E+05 | 2.21E+05 | 2.19E+05 | |
Ratio ofπ-10/actin | 0.065 ± 0.003 | 0.084 ± 0.004* | 0.102 ± 0.005** | 0.107 ± 0.005** | 0.082 ± 0.004* |