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Table 3 Effects of nano-TiO2on the amplification of cytokine mRNA of mouse by real-time PCR analysis after intraperitoneal injection with nano-anatase TiO2suspensions for consecutive 14 days

From: The Acute Liver Injury in Mice Caused by Nano-Anatase TiO2

Cytokine

Nano-anatase (mg/kg BW)

0

50

100

150

150-bulk

Refer-actin

Ct

21.035

19.93

21.4249

21.8257

21.038

Copies

2.68E+07

5.64E+07

2.06E+07

1.57E+07

2.68E+07

nf-κb

Ct

23.144

21.7009

22.4526

22.2415

23.0754

Relative copies

6.47E+06

1.71E+07

1.02E+07

1.19E+07

6.78E+06

Ratio ofnf-κb/actin

0.242 ± 0.012

0.303 ± 0.015*

0.497 ± 0.025**

0.756 ± 0.038**

0.253 ± 0.013

mif

Ct

22.4026

21.7153

21.5595

21.5625

20.5766

Relative copies

1.07E+07

1.70E+07

1.88E+07

1.88E+07

3.65E+07

Ratio ofmif/actin

0.398 ± 0.020

0.634 ± 0.032**

0.913 ± 0.046**

1.194 ± 0.060**

0.647 ± 0.032**

il-1β

Ct

24.2684

22.911

20.9252

22.2075

24.0418

Relative copies

3.04E+06

7.57E+06

2.89E+07

1.22E+07

3.54E+06

Ratio ofil-1β/actin

0.113 ± 0.006

0.481 ± 0.024**

0.511 ± 0.026**

0.590 ± 0.030**

0.132 ± 0.007*

il-6

Ct

25.076

22.6692

23.3343

22.8735

23.4873

Relative copies

1.76E+06

8.9lE+06

5.70E+06

7.77E+06

5.14E+06

Ratio ofil-6/actin

0.066 ± 0.003

0.158 ± 0.008**

0.276 ± 0.014**

0.494 ± 0.025**

0.192 ± 0.010**

crp

Ct

23.9738

21.7188

23.152

22.907

23.0768

Relative copies

3.70E+05

1.69E+07

6.44E+06

7.59E+06

6.77E+06

Ratio ofcrp/actin

0.138 ± 0.007

0.300 ± 0.015**

0.312 ± 0.016**

0.482 ± 0.024**

0.253 ± 0.013**

tnf

Ct

28.674

28.8403

28.659

28.3492

28.7297

Relative copies

1.56E+05

1.39E+05

1.58E+05

1.94E+05

1.50E+05

Ratio oftnf/actin

0.028 ± 0.001

0.052 ± 0.003**

0.076 ± 0.004**

0.123 ± 0.006**

0.056 ± 0.003

il-4

Ct

23.7878

22.2796

21.1993

21.3352

22.906

Relative copies

4.20E+06

1.16E+07

2.40E+07

2.19E+07

7.60E+06

Ratio ofil-4/actin

0.157 ± 0.008

0.205 ± 0.010**

0.895 ± 0.045**

1.392 ± 0.070**

0.369 ± 0.018**

π-10

Ct

28.5199

27.022

28.6367

28.1555

28.169

Relative copies

173E+05

4.75E+05

1.60E+05

2.21E+05

2.19E+05

Ratio ofπ-10/actin

0.065 ± 0.003

0.084 ± 0.004*

0.102 ± 0.005**

0.107 ± 0.005**

0.082 ± 0.004*

  1. Ranks marked with an asterisk or double asterisks means it is significantly different from the control (no nano-anatase or bulk TiO2) at the 5 or 1% confidence level, respectively. Values represent mean ± SE,n = 5

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