The Acute Liver Injury in Mice Caused by Nano-Anatase TiO2

Table 4 Effects of nano-Tio₂on the cytokine protein level of mice by ELISA analysis after intraperitoneal injection with nano-anatase TiO₂suspensions for consecutive 14 days

Nano-anatase (mg/kg BW)	NF-κB (ng/mL)	MIF (pg/mL)	IL-1β (pg/mL)	IL-6 (pg/mL)	CRP (ng/mL)	TNF-α (pg/mL)	IL-4 (pg/mL)	IL-10 (pg/mL)
0	1,819 ± 91	1,157 ± 58	482 ± 24	179 ± 9	103 ± 5	156 ± 8	1,079 ± 54	615 ± 31
5	2,434 ± 122*	1,341 ± 67*	599 ± 30*	202 ± 10*	153 ± 8**	190 ± 10**	1,216 ± 61*	773 ± 39*
10	3,852 ± 193**	1,529 ± 76**	669 ± 34**	303 ± 15**	204 ± 0**	226 ± 11**	1,304 ± 65*	1,617 ± 81**
50	4,511 ± 226**	1,789 ± 89**	736 ± 37**	422 ± 21**	266 ± 13**	294 ± 15**	1,399 ± 70*	1,869 ± 94**
100	5,738 ± 287**	2,326 ± 116**	848 ± 42**	541 ± 27**	327 ± 16**	346 ± 17**	1,490 ± 75**	2,081 ± 104**
150	6,819 ± 341**	3,098 ± 155**	979 ± 49**	699 ± 35**	428 ± 21**	596 ± 30**	1,717 ± 86**	2,403 ± 120**
150-bulk	3,838 ± 192**	1,401 ± 70*	612 ± 31*	391 ± 20**	175 ± 9**	202 ± 10**	1,330 ± 67*	1,027 ± 51*

Ranks marked with a an asterisk or double asterisks means it is significantly different from the control (no nano-anatase or bulk TiO₂) at the 5 or 1% confidence level, respectively. Values represent mean ± SE,n = 5