Figure 5

Kinetics and magnitude of ROS production in T cell and monocyte populations. Freshly isolated PBMC were treated with either 1 or 5 mM of 8-nm-sized ZnO NPs for 6 to 20 h. Following NP exposure, cells were loaded with the oxidation-sensitive DCFH-DA probe. Flow cytometry was then used to simultaneously evaluate ROS production and distinguish T cells and monocytes present in PBMC cultures by staining with fluorescently labeled CD3 and CD14 antibodies (a,c),n = 3. Parallel experiments were used to evaluate effects of NP treatment on cell viability by PI uptake and flow cytometry (b,d),n = 3.Error bars depict standard error