Figure 6

Quenching of ROS rescues T cells and monocytes from ZnO NP-induced cytotoxicity. Purified peripheral blood CD14⁺monocytes (≥96%purity) and CD4⁺T cells (≥95% purity) were pretreated for 4–6 h with 5 mMN-acetyl cysteine (NAC) or vehicle control and then subsequently cultured with 8-nm-sized ZnO NPs, or left untreated for 24 hr. Viability was assessed using the Alamar Blue cytotoxicity assay.a Purified monocytes treated with 0.125 mM ZnO NPs ± 5 mM NAC,n = 3.b Isolated CD4⁺T cells treated with 5 mM ZnO NPs ± 5 mM NAC,n = 3.Asterisks denote statistically significant differences (p < 0.05) as analyzed using a 2-way ANOVA anderror bars depict SE