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Table 1 NP association with immune cell subsets

From: The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction

 

Control (%/MFI)a

+ FITC-NP (%/MFI)b

CD3+T cells

1.79% ± 3.1%/1.15 ± 1.27

84.2% ± 4.1%/9.84 ± 3.23

CD4+T cells

1.61% ± 2.6%/1.23 ± 2.98

82.5% ± 5.9%/14.1 ± 7.21

CD19+B cells

2.0% ± 1.9%/2.85 ± 4.39

78.1% ± 3.1%/9.61 ± 5.11

CD14+Monocytes

1.7% ± 3.4%/5.80 ± 4.82

98.0% ± 2.5%/131.2 ± 20.11

  1. Freshly isolated PBMC were left untreated or exposed to 5 mM FITC-encapsulated ZnO NPs for 15 h. The cell mixture was then stained with fluorescently labeled CD3, CD4, CD19, CD14 antibodies to identify subsets, washed to remove excess antibody and unbound NPs, and the level of NP association determined based on FITC fluorescent signal using flow cytometry. Data were obtained by gating on 10,000 events,n = 3
  2. aValues shown represent the background autofluorescence signal for control cells (percent FITC positive cells/mean fluorescent intensity (MFI) ± SE)
  3. bThe percentage of FITC positive cells and MFI for cells treated with FITC-doped ZnO NP
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