Figure 6
Cells maintain normal morphology after exposure to magnetic nanospheres. Representative differential interference contrast (DIC) images of cells not exposed to magnetic nanospheres show typical morphology for PC12 cells (a) not treated or (b) treated daily with 100 ng/mL nerve growth factor (NGF) for 72 h. Normal morphology is maintained when cells are exposed to magnetic nanospheres and (c) not treated or (d) treated with NGF. Compared to cells not exposed to magnetic nanospheres and (e) not treated or (f) treated with NGF, cells exposed to magnetic nanospheres in the (g) absence or (h) presence of NGF have similar microtubule (green) and actin filament (red) cytoskeletal arrangements. Scale bar in (d) = 10 μm and is valid for (a) through (d). Scale bar in (h) = 10 μm and is valid for (e) through (h)