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Figure 1 | Nanoscale Res Lett

Figure 1

From: Atomic Force Microscopy Study of Protein–Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System

Figure 1

AFM images of non-monomerized (a) and (12% Emulgen 913)-monomerized (b) CYP11A1 molecules and density of distribution (ρ( h )) with height of non-monomerized and monomerized CYP11A1 (c). Tapping mode. Experimental conditions were as follows: 100 μM CYP11A1 non-monomerized and 100 μM CYP11A1 monomerized in 50 mM KP, pH 7.4, containing Emulgen 913 (12%). For AFM visualization, the samples were diluted to obtain 1 μM CYP11A1 in 50 mM KP with 0.5% Emulgen 913, pH 7.4, and immediately placed onto the mica surface. T = 25°C. Arrows (1) indicate the images of CYP11A1 aggregates, arrows (2) indicate the images of CYP11A1 monomers.

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