Atomic Force Microscopy Study of Protein–Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System

Table 1 The AFM heights (h_max) of distribution maximum of CYP11A1 images and the deaggregation degree (α) upon Emulgen 913 monomerization

Emulgen 913 concentration, %	h_max1, nm	h_max2, nm	% of monomers, α
CYP11A1
0	2.4 ± 0.3	3.8 ± 0.4	0
4	1.6 ± 0.2	2.8 ± 0.2	45 ± 4
8	1.6 ± 0.2	2.8 ± 0.2	70 ± 4
12	1.6 ± 0.2	2.6 ± 0.2	82 ± 4

α = N/N_tot is the AFM-measured deaggregation degree, where N is the number of molecules with the 1.6 ± 0.2 nm diameter; N_tot is the total number of molecules