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Table 2 The values of kon,koff and K D for the Ad/CYP11A1 monomeric and the Ad/CYP11A1 oligomeric complex formation reaction

From: Atomic Force Microscopy Study of Protein–Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System

  kon × 103 [M-1 s-1] koff [s-1] K D [μM]
Monomeric CYP11A1 290 ± 30 0.05 ± 0.005 0.17 ± 0.015
Oligomeric CYP11A1 420 ± 40 0.09 ± 0.009 0.21 ± 0.02
  1. Optico-biosensoric experiments were performed using a Biacore 3000 device. Approximately 400 RU of Ad were covalently immobilized on a carboxymethylated dextran matrix. Subsequently, different concentrations of CYP11A1 were passed over the flow cell. K D values were determined using the 1:1 binding mechanism available in the Biacore evaluation software 4.1