Atomic Force Microscopy Study of Protein–Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System

Table 3 AFM-measured heights of protein and protein complexes heights in CYP11A1 system

Name of protein or complex	AFM-measured object heights, nm
CYP11A1 monomer (M_r = 56 kDa)	1.4–2.8 with h_max = 1.6 ± 0.2
AdR monomer (M_r = 60 kDa)	1.4–2.2 with h_max = 1.8 ± 0.2
Ad monomer (M_r = 16 kDa)	0.8–1.8 with h_max = 1.0 ± 0.2
Ad + CYP11A1	1.3–2.6 with h_max = 1.6 ± 0.2
AdR/Ad	1.8–2.6 with h_max = 2.3 ± 0.2
AdR/CYP11A1	2.2–5.0 with h_max = 2.8 ± 0.2
AdR/Ad/CYP11A1	2.8–5.5 with h_max = 4.0 ± 1.0