Figure 2

AuNP loading has no significant effect on T cell viability or function. T cells were loaded in the presence of 0.5 nM AuNPs for 24 h or cultured in medium alone and then measured for viability using Annexin-V/PI staining (A), proliferation using thymidine incorporation (B), migration through a transwell membrane in response to tumor (LCL) produced supernatant (C) and intracellular analysis of IFN-γ cytokine production following mitogen stimulation (D).