Table 1 Experimental design matrix with observed values of the objectives variables of protein-loaded NPs
Experiment Code | X1 | X2 | X3 | Z-Avea (nm) | Polydispersibility Indice | E.E.(%) ± SD (n= 6) |
---|---|---|---|---|---|---|
1 | 8.2 | 5 | 0.66 | 280.8 | 0.483 | 35.29 ± 0.07 |
2 | 8.2 | 5 | 0.24 | 309.5 | 0.286 | 38.82 ± 0.53 |
3 | 8.2 | 2 | 0.66 | 602.6 | 0.702 | 51.76 ± 0.28 |
4 | 8.2 | 2 | 0.24 | 1428.7 | 0.632 | 50.59 ± 0.46 |
5 | 5.5 | 6 | 0.45 | 291.2 | 0.378 | 48.82 ± 0.12 |
6 | 5.5 | 1 | 0.45 | 890.7 | 0.806 | 54.71 ± 0.09 |
7 | 5.5 | 3.5 | 0.8 | 355.4 | 0.567 | 42.94 ± 0.14 |
8 | 5.5 | 3.5 | 0.1 | 640.2 | 0.686 | 55.88 ± 0.10 |
9 | 2.8 | 5 | 0.66 | 337.0 | 0.492 | 44.12 ± 0.74 |
10 | 2.8 | 5 | 0.24 | 345.8 | 0.384 | 45.88 ± 0.67 |
11 | 2.8 | 2 | 0.66 | 651.0 | 0.712 | 50.00 ± 0.41 |
12 | 2.8 | 2 | 0.24 | 2503.5 | 0.762 | 52.35 ± 0.75 |
13 | 10 | 3.5 | 0.45 | 380.5 | 0.536 | 44.71 ± 0.69 |
14 | 1 | 3.5 | 0.45 | 1025.5 | 0.691 | 49.41 ± 0.21 |
15 | 5.5 | 3.5 | 0.45 | 429.2 | 0.555 | 47.06 ± 0.55 |