Figure 1
From: bOptimizing atomic force microscopy for characterization of diamond-protein interfaces
Influence of cantilever spring constant on FBS layer characterization. TM AFM images of fetal bovine serum (FBS) on H/O-terminated monocrystalline diamond (MCD) in solution using cantilevers with different spring constants: (a) by CSG01 cantilever, NT-MDT, k = 0.06 N/m. Central area was scanned in CM (nanoshaving) across H/O-terminated diamond, the position of the boundary was determined using topography data from a large area 135 × 135 μm2 scan, actual scan size is 10 × 10 μm2, A 0 ~ 300 nm, energy of cantilever oscillations E ~ kA 2/2 ~ 10-15 J; (b) on O-diamond by Multi75Al cantilever, Budget Sensors, k = 3 N/m, scan size 1 × 1 μm2, A 0 ~ 50 nm, E ~ 10-14 J; (c) on O-diamond by NaDiaProbes, ADT, k = 120 N/m, scan size 15 × 15 μm2, A 0 ~ 50 nm, E ~ 10-13 J, proteins were removed from the central area of 5 × 5 μm2 by CM scan prior to TM.