Figure 2
From: bOptimizing atomic force microscopy for characterization of diamond-protein interfaces
Wear of protein layers in tapping mode AFM. AFM image of FBS layer on monocrystalline diamond (MCD) in McCoy medium using NSG01 tip (NT-MDT): (a) The protein layer was removed from 2 × 2 μm2 area in CM (F ~ 600 nN). (b) The protein layer was partially removed from 4 × 4 μm2 area using 5 scans in TM (A 0 ~ 60 nm, E ~ 10-15 J). (c) The protein layer was measured by TM, scan 10 × 10 μm2 (A 0 ~ 60 nm). (d) FBS layer on MCD was studied in CM and TM in air using NSG01 tip. FBS layer was removed after just 2 scans with the highest amplitude (A 0 ~ 700 nm, E ~ 10-12 J). (e) The scheme illustrates the TM nanoshaving experiment as shown in (a-c). (f) Wear rate dependence on free amplitude and oscillation energy for TM nanoshaving of FBS layer adsorbed on MCD using medium k (NSG01) cantilevers, r SP = 0.5.