- Nano Express
- Open Access
PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells
- Gao Feng Liang†1,
- Yan Liang Zhu†1,
- Bo Sun1,
- Fei Hu Hu1,
- Tian Tian1,
- Shu Chun Li1 and
- Zhong Dang Xiao1Email author
© Liang et al; licensee Springer. 2011
- Received: 14 March 2011
- Accepted: 12 July 2011
- Published: 12 July 2011
The biggest challenge in the field of gene therapy is how to effectively deliver target genes to special cells. This study aimed to develop a new type of poly(D,L-lactide-co-glycolide) (PLGA)-based nanoparticles for gene delivery, which are capable of overcoming the disadvantages of polyethylenimine (PEI)- or cationic liposome-based gene carrier, such as the cytotoxicity induced by excess positive charge, as well as the aggregation on the cell surface. The PLGA-based nanoparticles presented in this study were synthesized by emulsion evaporation method and characterized by transmission electron microscopy, dynamic light scattering, and energy dispersive spectroscopy. The size of PLGA/PEI nanoparticles in phosphate-buffered saline (PBS) was about 60 nm at the optimal charge ratio. Without observable aggregation, the nanoparticles showed a better monodispersity. The PLGA-based nanoparticles were used as vector carrier for miRNA transfection in HepG2 cells. It exhibited a higher transfection efficiency and lower cytotoxicity in HepG2 cells compared to the PEI/DNA complex. The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes. Moreover, nanocomplex shows better serum compatibility than commercial liposome. PLGA nanocomplexes obviously accumulate in tumor cells after transfection, which indicate that the complexes contribute to cellular uptake of pDNA and pronouncedly enhance the treatment effect of miR-26a by inducing cell cycle arrest. Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.
- HepG2 Cell
- Gene Delivery
- Energy Dispersive Spectroscopy
- Transfection Efficiency
- PLGA Nanoparticles
MicroRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression at the post-transcriptional level. They involve in various cellular mechanisms including development, differentiation, proliferation, and apoptosis. The pivotal roles of these miRNAs in human cancers have been discovered [1, 2], and the therapeutic applications of miRNA have been developed using various viral vectors [3, 4].
However, the disadvantages of viral vectors limited their application in gene delivery, such as immunogenic/inflammatory responses, low loading capacity, large scale manufacturing, and quality control . Consequently, more attention have been paid on non-viral gene delivery vectors in recent years, such as liposomes (lipoplexes), polycationic polymers (polyplexes), and organic or inorganic nanoparticles (nanoplexes) . To enhance gene delivery effect, various cationic complexes have been developed for delivering plasmid DNA, antisense, or siRNA into cells [7–9]. Poly(D,L-lactide-co-glycolide) (PLGA) were extensively assessed for their ability of delivering variety of therapeutic agents [10–12]. PLGA nanoparticles were shown to escape from the endo-lysosomal compartment to the cytoplasmic compartment and release their contents over extended periods of time . These features rendered PLGA nanoparticles as potential tool for gene delivery efficiently.
Polyethylenimine (PEI) is water-soluble, linear, or branched polymers with a protonable amino group [14, 15]. Due to their high cationic charge density at physiological pH, PEIs are able to form non-covalent complexes with DNA, siRNA, and antisense oligodeoxynucleotide. Therefore, PEIs hold a prominent position among the polycationic polymers used for gene delivery [16–18]. The intracellular release of PEI/nucleic acids complexes from endosomes is considered as relying on the protonation of amines in the PEI molecule, which leading to osmotic swelling and subsequent burst of the endosomes. Moreover, PEIs also facilitate nucleic acid entry into the nucleus [19, 20]. However, it has been reported that long PEI chains are highly effective in gene transfection, but more cytotoxic [14, 21, 22].
In order to overcome these hurdles in gene therapy and improve gene delivery efficiency, we developed novel non-liposome-based cationic polymers which are composed of PLGA as the core and cationic PEI as the shell. The biodegradable PLGA nanoparticles, modified with a polyplexed PEI coating, were tested by loading the expression vector (pDNA) of miR-26a, which is capable of inducing cell cycle arrest in HepG2 cells. In this study, nanoparticles of controlled size and persistent shape have been obtained by an emulsion evaporation method and characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), and energy dispersive spectroscopy (EDS). The nanoparticles have been determined by their physicochemical and biological properties. The formulated nanoparticles enhance cellular uptake of miRNA, pronounce upregulation of miR-26a, induce cell cycle arrest, and improve gene expression activity compared with PEI and commercial liposome. Furthermore, these particles can be easily fabricated and have a high transfection efficiency and low cell toxicity. Our results suggest a new approach for miRNA delivering by PLGA/PEI nanoparticles in gene therapy.
Branched PEI (M W, 25 kDa) and poly(vinylalcohol) (PVA) were obtained from Sigma Aldrich (St. Louis, MO, USA). D,L-Lactide/glycolide copolymer (PLGA, lactic/glycolic molar ratio: 53/47; M W, 25 kDa) was purchased from Daigang Chemical Reagent Co., Ltd. (Jinan City, Shandong Province, China). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, trypsin, and Dulbecco's PBS were purchased from Invitrogen (Carlsbad, CA, USA), and pGFP-miRNA plasmid was constructed according to the methods described previously . Other reagents were of analytical grade obtained from suppliers and used without purification.
PLGA/PEI nanosphere synthesis
PLGA nanospheres were obtained by using water-in-oil-in-water solvent evaporation technique as described previously . Briefly, 150 mg of PLGA polymer was dissolved in 1.5 ml of dichloromethane to yield a 10% (w/v) polymer solution. After 3 ml of a 7% (w/v) aqueous solution of PVA was added to the organic phase and emulsified at 10,000 × g using a homogenizer for 5 min. The resulting double emulsion was then poured into 50 ml of a 1% PVA solution and emulsified for 15 min. This resulted in the formation of a water/oil/water emulsion that was stirred for at least 12 h at room temperature, allowing the methylene chloride to evaporate. The resulting microspheres were washed twice in deionized water by centrifugation at 16,000 × g and freeze-dried.
Then, PEI aqueous solution was added in the aforementioned PLGA nanoparticle suspension, and incubated fifteen minutes.
PLGA nanoparticles, PLGA/PEI nanoparticles, and PLGA/PEI /pDNA complexes mean hydrodynamic diameters measurements were conducted by using Nano Particle Analyzer (Beckman Coulter, Fullerton, CA, USA). The mean hydrodynamic diameter was determined via cumulative analysis.
The zeta-potential (surface charge) of the polymers and polyplexes was determined at 25°C with a scattering angle of 90° using potential measurement analyzer (90 PLus, Brookhaven, Holtsville, NY, USA). Samples were prepared in PBS and diluted with deionized water to ensure that the measurements be performed under conditions of low ionic strength where the surface charge of the particles can be measured accurately.
The particle size and morphology of the PLGA nanoparticles, PEI-modified PLGA nanoparticles, and PEI-modified PLGA nanoparticles/miRNA complexes were characterized via transmission electron microscope (TEM, JEM-2100, JEOL, Tokyo, Japan).
Energy dispersive spectroscopic analysis (Oxford EDS, Oxford Instruments, Oxon, UK) was employed to perform the quantitative elemental analysis of the nanocomplex.
Measurement of the interactions between miRNA and nanoparticles
Complexes were formed by diluting miRNA expression vector (pDNA) and the different amount of nanoparticles separately with 0.9% NaCl (pH 7.4). The nanoparticles at different concentrations were added to 1 μg green fluorescent protein(pPG-eGFP-miR) (GFP)-encoded miRNA vector solution, vortexed immediately at room temperature, and allowed to stand for 30 min to form PLGA/PEI/pDNA complexes. Then, the complexes were submitted to electrophoresis in 1%/TAE agarose gel at 90 V for 60 min. Images were acquired using a PeiQing gel imaging system (PeiQing, Shanghai City, China). GelRed (Biotium, Hayward, CA, USA), an ultra sensitive nucleic acid dye, was used to examine the interactions of DNA with the nanocomplex to determine optimal N/P ratio of the nanocomplex .
HepG2 (human hepatocellular carcinoma cell) cells were cultured in DMEM supplemented with 10% FBS, streptomycin at 100 μg/mL, and penicillin at 100 U/mL. Cells were maintained at 37°C in humidified and 5% CO2 incubator. The cytotoxicity of PLGA/PEI nanoparticles, complexed with or without pDNA, was determined in a separate set of experiments using MTT assay to detect changes in cell viability after an incubation time of 24 h. Cells were seeded in 24-well plates at an initial density of 2 × 104 cells/well for HepG2 in 0.5 mL of growth medium and incubated for 24 h prior to the addition of PLGA/PEI and PLGA/PEI/DNA at different N/P ratio. Untreated cells were taken as control with 100% viability. Triton X-100 1% (SPI Supplies, West Chester, PA, USA) was used as positive control of cytotoxicity.
In vitro gene transfection and quantification study
Before GFP transfection assay, cells were seeded in 24-well plates at a density dependent of the cell line in DMEM with 10% FBS. When the cells were at 50% to 70% confluence, the medium in each well was replaced with fresh normal medium or medium containing naked pGFP, lipo2000, or PLGA/PEI/DNA complex under standard incubator conditions. After 48 h, cells harboring an expressing integrant were viewed by fluorescence microscopy based on GFP.
The analysis of transfection efficiency was performed using a flow cytometer (BD Biosciences, Mountain View, CA, USA). Cells were first washed with PBS and detached with 0.2 mL of 0.25% trypsin. Growth medium was then added, and the cells suspension was centrifuged at 1,000 rpm for 5 min. Two further cell-washing cycles of resuspension and centrifugation was carried out in PBS before fixation in 0.4 mL of 75% ethanol. The percentage of cells expressing GFP was then determined from 10,000 events and reported as a mean ± standard deviation (SD) of three samples.
miRNA expression and cell cycle study
Gene-specific primers used to amplify the miRNAs
Forward primer (5' → 3')
Reverse primer (5' → 3')
Then, real-time quantitative polymerase chain reaction (PCR) was performed to evaluate miR-26a expression in HepG2 cells after 3 days transfection using standard protocols on an Applied Biosystems 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). Briefly, 1.25 μl of cDNA was added to 10 μl of the 2 × SYBR green PCR master mix (TaKaRa, Dalian, China), 200 nM of each primer and water to 20 μl. The reactions were amplified for 15 s at 95°C and 1 min at 60°C for 40 cycles. The thermal denaturation protocol was run at the end of the PCR to determine the number of the products that were present in the reaction. Reactions are typically run in triple. The cycle number at which the reaction crossed an arbitrarily placed threshold (C t) was determined for each gene and the relative amount of each miRNA. Target gene expression was normalized to the expression of the housekeeping gene U6 for each sample. Data were analyzed using the method .
Cell cycle profiles of nanoparticles transfected HepG2 cells were performed using a FACS Calibur and with CellQuest™ software (BD Biosciences, Mountain View, CA, USA). HepG2 cells (5 × 104 per well) were seeded into 24-well plates. Cells were treated with different formulations at a concentration of 1 μg miRNA in serum containing medium at 37°C for 72 h. Cells were washed once with PBS and then fixed in 75% ethanol and, after fixation, stained with PI according to manufacturer's instructions.
All statistical analyses were performed by Student's t test. Data were expressed as means ± SD. Results were considered statistically significant when the p value was less than 0.05.
Particle size and surface morphology of the nanoparticles
Particle size and zeta potential have been demonstrated to play important roles in determining the level of cellular and tissue uptake; researchers have also conducted to investigate the size effect on the gene delivery efficiency. PLGA nanoparticles with smaller size below 100 nm have been proved to gain higher gene transfection efficiency than those of 200 nm [28, 29].
Size distribution, zeta potential, and elementary analysis of the nanoparticles
Type of formulation
Zeta potential (mV)
Atom% - O
Atom% - N
Atom% - P
50 ± 6
-21.4 ± 1.8
49% (± 0.74)
0% (± 0)
0% (± 0)
57 ± 7
29.4 ± 2.6
42% (± 0.63)
4.7% (± 0.32)
0% (± 0)
PLGA-PEI/pDNA(N/P = 6)
60 ± 7
23.7 ± 2.3
41% (± 0.98)
4.1% (± 0.43)
1.1% (± 0.13)
EDS is capable of providing both qualitative and quantitative information about the presence of different elements in nanoparticles. In this study, the surface modification of PLGA nanospheres with PEI or PEI/pDNA was confirmed by EDS. Because PEI contains the nitrogen element and pDNA contains phosphorus element, but PLGA contains neither of them, the nitrogen that is detected in PLGA/PEI nanoparticles is an evidence for the existence of PEI on the PLGA nanoparticles surface (Table 2). By detecting of phosphorus PLGA/PEI/pDNA nanocomplex, we have shown that pDNA has been successfully adsorbed on the PLGA/PEI surface. From the combination of the aforementioned data, it can be inferred that the PEI or pDNA is completely complexed with the nanoparticles by ionic binding.
Characterization of nanoparticles/pDNA complexes
In vitro gene expression assay
Next, transfection efficiency of the nanoparticles was compared to other different vehicle. As shown in Figure 3, GFP expression is hardly detected when transfection was mediated by naked pDNA, which was used as negative control. The results have demonstrated that the naked GFP is difficult to be directly internalized by the cells. In contrast, GFP-contained nanoparticles facilitate the cell endocytosis. Figure 3E also showed that transfection efficiency of nanoparticles is comparable to the commercial liposomes, and obviously better than the sole PEI particles.
Furthermore, PLGA/PEI nanoparticles display the transfection efficiency in a dose-dependent manner (Figure 3F). The uptake efficiency of nanoparticles by cells, assayed on 48 h after incubation, was higher at lower nanoparticle concentration. However, the transfection efficiency deceased when the nanoparticle concentration is further increasing due to the potential cytotoxicity.
Furthermore, we studied the stability of nanoparticles/pDNA in serum. Results suggested that transfection efficiency of the nanocomplexes is higher than lipo2000 in the presence of serum (data not shown). Meanwhile, the GFP expression at the 48 h of incubation was significantly higher than at 24 h. While the nanoparticle/DNA in the medium was washed away at 24 h after transfection, after another 24 h, GFP signals were not obviously enhanced (data not shown). The data indicate that the increasing GFP expression results from the sustained nanoparticle uptake effect of the transfected cells. It was further proved that the nanoparticles have good biocompatibility in serum.
In vitro cytotoxicity
miR-26 expression and cell cycle induction
Numbered cell cycle corresponding to Figure 6
Researchers have reported that HMW cationic PEI as a transfection reagents showed higher cytotoxicity [21, 22, 31, 32]. In our studies, the formulation of PLGA-based nanoparticle significantly reduces the cytotoxicity of the PEI. We have demonstrated that PLGA/PEI nanocomplex shows higher gene transfection efficiency and better serum compatible than Lipofectamine2000 or PEI. As for the possible reasons, we speculated that the enhanced transfection effect may be related to the interaction between PLGA and PEI. In the given nanoparticles mentioned above, PLGA showed a better biocompatibility than the lipids. Obviously, the amino groups of PEI play an important role in determining the biological characteristics of the nanocomplexes. To our knowledge, there are few scientific articles describing solid preformed PEI-based nanoparticles and their cellular applications. Moreover, in the most of these studies, PEI is complexed through cooperative electrostatic interactions with other anionic polymers or converted into nanoparticles by introducing ionic and covalent crosslinkers without any addition of other polymers.
Furthermore, the properties of the nanoparticles we prepared can be easily controlled. Their size can be tuned by modulating experimental conditions of preparation, and the surface charge can be adjusted by changing the PEI amount introduced into the complex. Finally, cytotoxicity studies showed that the PLGA/PEI/DNA complex exhibited less cytotoxicity than HMW PEI and liposome. In the tested cell line, the transfection efficiencies mediated by PLGA/PEI increased with the increase of nanoparticles/DNA NP ratio in the presence or absence of serum.
In this study, we developed a PLGA-based nanoparticles polyplexed with miRNA expression vector as a potential approach to deliver genes with non-viral vectors. The biodegradable nanoparticles can efficiently deliver nucleic acid to human hepatocellular carcinoma cells and enhance the effect of functional miRNA delivered, such as the cell cycle suppressor miRNA26a. Importantly, it is proved that serum cannot inhibit the transfection activity of this nanoparticle. These PLGA-based nanoparticles also display an improved safety profile in comparison with high molecular weight PEIs and liposome because of the lower cytotoxicity of the polyplex formulations. This study presents an effective gene delivery vehicle, PLGA-based nanoparticle, which may contribute to the gene therapy for tumor and other miRNA-related diseases such as diabetic, cardiovascular disease, and neurodegenerative diseases.
The authors are grateful for financial support fromthe National Basic Research Program of China (973 Program: 2007CB936300), NSFC (no. 20875014, no. 30870626), and 2008DFA51180.
- Esquela-Kerscher A, Slack FJ: Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer 2006, 6: 259. 10.1038/nrc1840View ArticleGoogle Scholar
- Pineau P, Volinia S, McJunkin K, Marchio A, Battiston C, Terris B, Mazzaferro V, Lowe SW, Croce CM, Dejean A: miR-221 overexpression contributes to liver tumorigenesis. Proc Natl Acad Sci USA 2010, 107: 264. 10.1073/pnas.0907904107View ArticleGoogle Scholar
- Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, Mendell JT: Therapeutic microRNA Delivery Suppresses Tumorigenesis in a Murine Liver Cancer Model. Cell 2009, 137: 1005. 10.1016/j.cell.2009.04.021View ArticleGoogle Scholar
- Yang XA, Haungot V, Zhou SZ, Luo GX, Couto LB: Inhibition of Hepatitis C Virus Replication Using Adeno-Associated Virus Vector Delivery of an Exogenous Anti-Hepatitis C Virus MicroRNA Cluster. Hepatology 2010, 52: 1877. 10.1002/hep.23908View ArticleGoogle Scholar
- Itaka K, Kataoka K: Recent development of nonviral gene delivery systems with virus-like structures and mechanisms. European J Pharm Biopharm 2009, 71: 475. 10.1016/j.ejpb.2008.09.019View ArticleGoogle Scholar
- Kumar M, Sameti M, Mohapatra SS, Kong X, Lockey RF, Bakowsky U, Lindenblatt G, Schmidt H, Lehr CM: Cationic silica nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency in vitro and in vivo. J Nanosci Nanotech 2004, 4: 876. 10.1166/jnn.2004.120View ArticleGoogle Scholar
- Lechardeur D, Lukacs GL: Intracellular barriers to non-viral gene transfer. Curr Gene Ther 2002, 2: 183. 10.2174/1566523024605609View ArticleGoogle Scholar
- Geusens B, Lambert J, De Smedt SC, Buyens K, Sanders NN, Van Gele M: Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes. J Control Release 2009, 133: 214. 10.1016/j.jconrel.2008.10.003View ArticleGoogle Scholar
- Han SE, Kang H, Shim GY, Suh MS, Kim SJ, Kim JS, Oh YK: Novel cationic cholesterol derivative-based liposomes for serum-enhanced delivery of siRNA. Intl J Pharm 2008, 353: 260.Google Scholar
- Panyam J, Labhasetwar V: Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Delivery Rev 2003, 55: 329. 10.1016/S0169-409X(02)00228-4View ArticleGoogle Scholar
- Perez C, Sanchez A, Putnam D, Ting D, Langer R, Alonso MJ: Poly(lactic acid)-poly(ethylene glycol) nanoparticles as new carriers for the delivery of plasmid DNA. J Control Release 2001, 75: 211. 10.1016/S0168-3659(01)00397-2View ArticleGoogle Scholar
- Ma YD, Zheng Y, Liu KX, Tian G, Tian Y, Xu L, Yan F, Huang LQ, Mei L: Nanoparticles of Poly(Lactide-Co-Glycolide)-d-a-Tocopheryl Polyethylene Glycol 1000 Succinate Random Copolymer for Cancer Treatment. Nanoscale Res Lett 2010, 5: 1161. 10.1007/s11671-010-9620-3View ArticleGoogle Scholar
- Stern M, Ulrich K, Geddes DM, Alton E: Poly (D, L-lactide-co-glycolide)/DNA microspheres to facilitate prolonged transgene expression in airway epithelium in vitro, ex vivo and in vivo. Gene Ther 2003, 10: 1282. 10.1038/sj.gt.3301994View ArticleGoogle Scholar
- Godbey WT, Wu KK, Mikos AG: Size matters: Molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. J Biomed Mater Res 1999, 45: 268. 10.1002/(SICI)1097-4636(19990605)45:3<268::AID-JBM15>3.0.CO;2-QView ArticleGoogle Scholar
- Tang MX, Szoka FC: The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther 1997, 4: 823. 10.1038/sj.gt.3300454View ArticleGoogle Scholar
- Jiang G, Park K, Kim J, Kim KS, Hahn SK: Target Specific Intracellular Delivery of siRNA/PEI-HA Complex by Receptor Mediated Endocytosis. Mol Pharm 2009, 6: 727. 10.1021/mp800176tView ArticleGoogle Scholar
- Boussif O, Lezoualch F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP: A versatile vector for gene and oligonucleotide transfer into cells in culture and in-vivo - polyethylenimine. Proc Natl Acad Sci USA 1995, 92: 7297. 10.1073/pnas.92.16.7297View ArticleGoogle Scholar
- Zhang M, Xue YN, Liu M, Zhuo RX, Huang SW: Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors. Nanoscale Res Lett 2010, 5: 1804. 10.1007/s11671-010-9716-9View ArticleGoogle Scholar
- Godbey WT, Wu KK, Mikos AG: Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci USA 1999, 96: 5177. 10.1073/pnas.96.9.5177View ArticleGoogle Scholar
- Behr JP: A trick to enter cells the viruses did not exploit. Chimia 1997, 51: 34.Google Scholar
- Fischer D, Bieber T, Li YX, Elsasser HP, Kissel T: A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: Effect of molecular weight on transfection efficiency and cytotoxicity. Pharm Res 1999, 16: 1273. 10.1023/A:1014861900478View ArticleGoogle Scholar
- von Harpe A, Petersen H, Li YX, Kissel T: Characterization of commercially available and synthesized polyethylenimines for gene delivery. J Control Release 2000, 69: 309. 10.1016/S0168-3659(00)00317-5View ArticleGoogle Scholar
- Liang GF, Chen SS, Zhu YL, Li SC, Xiao ZD: Construction of MiRNA Eukaryotic Expression Vector and its Stable Expression in Human Liver Cancer Cells. 3rd Intl Conference Bioinform Biomed Tech 2011., 30: Google Scholar
- Kumar M, Bakowsky U, Lehr CM: Preparation and characterization of cationic PLGA nanospheres as DNA carriers. Biomaterials 2004, 25: 1771. 10.1016/j.biomaterials.2003.08.069View ArticleGoogle Scholar
- Choosakoonkriang S, Lobo BA, Koe GS, Koe JG, Middaugh CR: Biophysical characterization of PEI/DNA complexes. J Pharm Sci 2003, 92: 1710. 10.1002/jps.10437View ArticleGoogle Scholar
- Chen CF, Ridzon DA, Broomer AJ, Zhou ZH, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 2005, 33: e179. 10.1093/nar/gni178View ArticleGoogle Scholar
- Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 2001, 25: 402. 10.1006/meth.2001.1262View ArticleGoogle Scholar
- Kim JH, Park JS, Yang HN, Woo DG, Jeon SY, Do HJ, Lim HY, Kim JM, Park KH: The use of biodegradable PLGA nanoparticles to mediate SOX9 gene delivery in human mesenchymal stem cells (hMSCs) and induce chondrogenesis. Biomaterials 2011, 32: 268. 10.1016/j.biomaterials.2010.08.086View ArticleGoogle Scholar
- Prabha S, Zhou WZ, Panyam J, Labhasetwar V: Size-dependency of nanoparticle-mediated gene transfection: studies with fractionated nanoparticles. Intl J Pharm 2002, 244: 105. 10.1016/S0378-5173(02)00315-0View ArticleGoogle Scholar
- Xia T, Kovochich M, Liong M, Zink JI, Nel AE: Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways. Acs Nano 2008, 2: 85. 10.1021/nn700256cView ArticleGoogle Scholar
- Fukumoto Y, Obata Y, Ishibashi K, Tamura N, Kikuchi I, Aoyama K, Hattori Y, Tsuda K, Nakayama Y, Yamaguchi N: Cost-effective gene transfection by DNA compaction at pH 4.0 using acidified, long shelf-life polyethylenimine. Cytotechnology 2010, 62: 73. 10.1007/s10616-010-9259-zView ArticleGoogle Scholar
- Moghimi SM, Symonds P, Murray JC, Hunter AC, Debska G, Szewczyk A: A two-stage poly(ethylenimine)-mediated cytotoxicity: Implications for gene transfer/therapy. Mol Ther 2005, 11: 990. 10.1016/j.ymthe.2005.02.010View ArticleGoogle Scholar
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