Figure 3

Nanostructure on the surface of immature and mature BMDCs. (A-N) AFM was adopted to determine nanostructures of the immature BMDCs treated with 10.0 μg/L of GM-CSF plus 10.0 μg/L of IL-4 (A-G) or the mature BMDCs stimulated with 1.0 mg/L of LPS (H-N), and to make quantitatively analysis for them; A and H, multiple immature BMDCs (A) or mature BMDCs (H) at lower resolution; B and I, three-dimensional images respectively from the black line-circled cells on A and H images; C and J, height profiles alone the black lines (b1 and i1) drawn across the cells on B and I images, respectively; D and K, single immature BMDC in scanning area of 18 × 18 μm (D) or single mature BMDC in scanning area of 30 × 30 μm (K) respectively from the black line-circled cells on A and H images; E and L, Enlarged view of the protrusion or pseudopodia on the edge of the immature BMDCs (E) in the scanning size of 3 × 3 μm and the mature BMDCs (L) in the scanning size of 3 × 3 μm; F and M, Enlarged view of the center of the immature BMDCs (F) and the mature BMDCs (M) in the same scanning area of 5 × 5 μm; G and N, histograms of the particles of the immature BMDCs (G) and the mature BMDCs (N).