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Figure 7 | Nanoscale Research Letters

Figure 7

From: Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population

Figure 7

Concentration- and time-dependent effects of nanoparticles on the GFP expression of pIL8-GFP A549 cells. (a) pIL8-GFP A549 cells were cultured in the microcavities and exposed to 30 μg/ml GC10 and SC10 for 48 h. The GFP expression of the pIL8-GFP A549 cells was analyzed via fluorescence time-lapse microscopy. The percentage of GFP-expressing cells was quantified using the software analysis. (b) pIL8-GFP A549 cells were treated with 0 to 50 μg/ml SC10 for 24 h in the microcavity under physiological conditions. Parallel 10,000 pIL8-GFP A549 cells were cultured and treated in a 96-well microplate with 0 to 50 μg/ml SC10. The GFP expression of the pIL8-GFP A549 cells in the microcavities was analyzed by fluorescence microscopy and the GFP expression of the cells in the microplate by fluorescence spectrometry.

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