Gold and silver nanoparticles for biomolecule immobilization and enzymatic catalysis

Table 2 Characterizations of TbADH and NADPH immobilized on Au-( n %)glut ^a nanoparticles

Glutaraldehyde ( n %)	Enzyme loading (mg/mL) (%)	Activity expressed (U/mL)	Specific activity (U/mg)	C_NADPH(mM)
Free TbADH	-	0.5	12.5	-
Free NADPH	-	-	-	1.6
0.3	1.8 (88)	0.23	5.8	1.2
0.5	1.8 (91)	0.27	6.2	1.23
0.7	1.8 (92)	0.36	9.0	1.26
1.0	1.9 (96)	0.35	8.7	1.25
1.4	1.8 (92)	0.13	2.6	1.2
1.8	1.8 (92)	0.1	2.6	1.2

^aThe mean size of Au nanoparticles was 15 nm, and n is the percent of glutaraldehyde solution used in the treatment.