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Table 2 RCCD consisting of three experimental factors in coded and actual levels with experimental results

From: Preparation and optimization of matrix metalloproteinase-1-loaded poly(lactide-co-glycolide-co-caprolactone) nanoparticles with rotatable central composite design and response surface methodology

Formulation Coded independent variables Actual independent variables Dependent variables
X 1 X 2 X 3 X 1 X 2 X 3 EE (%) Mean size
1 1 1 1 10 5.2 0.42 36.96 381.2
2 1 1 −1 10 5.2 0.18 28.12 321.1
3 1 −1 1 10 2.8 0.42 42.74 510.2
4 1 −1 −1 10 2.8 0.18 41.09 770.2
5 −1 1 1 4 5.2 0.42 49.08 426.3
6 −1 1 −1 4 5.2 0.18 44.67 371.9
7 −1 −1 1 4 2.8 0.42 61.98 806.7
8 −1 −1 −1 4 2.8 0.18 54.10 1,216.6
9 +1.682 0 0 12 4 0.3 20.14 420.6
10 −1.682 0 0 2 4 0.3 60.50 790.1
11 0 +1.682 0 7 4 0.3 32.38 362.7
12 0 −1.682 0 7 2 0.3 50.14 980.1
13 0 0 +1.682 7 4 0.5 47.22 522.2
14 0 0 −1.682 7 4 0.1 32.19 539.8
15 to 20 0 0 0 7 4 0.3 37.38 471.1
  1. n = 3. EE, entrapment efficiency; RCCD, rotatable central composite design; X1, duration time of homogenization; X2, agitation speed of homogenization; X3, volume ratio of organic solvent phase to external aqueous phase.