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Figure 4 | Nanoscale Research Letters

Figure 4

From: DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers

Figure 4

Optical response of HRP-modified porous SiO 2 to enzymatic reaction products infiltrating into the nanostructure. The HRP-modified PSiO2 nanostructure is pretreated with 50-mM PBS (pH 7.4) to minimize nonspecific adsorption of proteins. (a) Wash with 0.8-mM 4-chloro-1-naphthol in PBS buffer (pH 7.4). (b) Addition of H2O2 to the cycled solution. The PSiO2 biosensor is fixed in a custom-made flowcell, and the reflectivity spectra are recorded every 30 s. Inset: oxidation of 4-chloro-1-naphthol in the presence of HRP and H2O2.

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