Figure 4

Location of PEI–pEGFP or PEI–DCN in injected glomeruli. At 48 h after injection of the MC vector with PEI– pEGFP or PEI– DCN, paraffin sections of kidney were prepared and examined by immunohistochemistry. (A) Normal control glomerulus negative for EGFP staining. (B) Glomerulus of injected kidney with PEI– pEGFP expressing MC, the results show EGFP-positive cells are located mainly in the mesangial area and a few epithelial cells of the proximal tubule. (a,b ABC, ×100) (C) Glomerulus of injected kidney with PEI– DCN expressing MC. The exogenous DCN-positive cells have the same localization as EGFP in glomeruli. (D) Glomerulus of injected kidney with PEI– DCN expressing MC. The vimentin-positive MC are distributed mainly in the mesangium of glomeruli, which is consistent with the distribution pattern of PEI-DCN positive cells (c,d ABC × 200).