Table 1 Primers used in real-time qPCR analyses
From: Molecular response of Escherichia coli adhering onto nanoscale topography
| Gene name | Primer forward | Codified protein | GenInfo identifier |
|---|---|---|---|
| Primer reverse | |||
| gapA | TTGGCCGTATCGGTCGCATT | Glyceraldehyde-3-phosphate dehydrogenase A | 16129733 |
| AAACGGCCGTGAGTGGAGTC | |||
| ompC | TAACGGCGACCGTGCTGAAA | Outer membrane porinprotein C | 16130152 |
| ACGCGAGTTGCGTTGTAGGT | |||
| luxS | ACCGTGTTCGATCTGCGCTT | S-ribosylhomocysteinelyase | 16130599 |
| GCAAACAGGTGCTCCAGGGT | |||
| murA | AACGAAGCTCCAGGGCGAAG | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | 16131079 |
| TTCGCACCCAGCTGGCTTAG | |||
| fliC | CAGTTCTCCAACCGCGGTCA | Flagellar filament structural protein (flagellin) | 16129870 |
| GAGCCTCACCACCAGCAGTC | |||
| lpxC | GCAACCAGCGCTATGCGATG | UDP-3-O-acyl N-acetylglucosaminedeacetylase | 16128089 |
| AGGCACAAACCACGGGACTG | |||
| slp | CCGACTGCTCGCCAGACAAA | Outer membrane lipoprotein | 90111603 |
| CACACCTGGATGCCCTGCAT | |||
| flu | TGCCGGCACGGTCCGGGATGA | CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter | 49176177 |
| GCCCCGGGCGCGGAAGTCGT | |||
| cpxP | GGCCCGGCACGAACAGCCTCCT | Periplasmic adaptor protein | 49176443 |
| ACCGCTTGCTGCTCCGGCGT | |||
| dsbA | CACAAGGCCGGGGCGCGTGG | Oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I | 49176085 |
| AGCGCAGCACCCAGAACGCCGA | |||
| degP | GCGCTGGGGCGTAGCGGCCT | Serine endoprotease (protease Do), membrane-associated | 16128154 |
| TGCCGCCGTCCGGTGCGAGG | |||
| cpxR | TCTGGCTGACGCTGGCGCTGGT | Sensory histidine kinase/signal sensing protein | 16129840 |
| CGCCCGGAACAGACGCCGCCA | |||
| fimE | GTTACGGGGCAACGGGAGCC | Tyrosine recombinase/inversion of on/off regulator of fimA | 16132134 |
| CTGGGTCCAGCGTTCCACGG |