Figure 2
From: Statistical analysis of immuno-functionalized tumor-cell behaviors on nanopatterned substrates
Optical and fluorescence images of A549 cells. (a) Flourescence images of A549 cells cultured on three different substrates (planar glass, QNP, and SiNW arrays) at 37°C for 0.5, 21, and 45 h. (b) Optical and fluorescence images of the immobilized A549 cells on STR-functionalized QNP arrays after 21 h of incubation at 37°C (marked area A in the second row of (a)). Before fluorescent imaging, the actin filaments were stained with Alexa Fluor 594 conjugated phalloidin (Invitrogen, green 532 nm) for 20 min, and the nucleus was stained with DRAQ-5 (Cell Signaling, red 635 nm).