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Table 1 Sequences of oligonucleotides employed in this work

From: Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimurium

Name

Sequence

A1: anti-E. coli O157:H7 aptamer

5-CCGGACGCTTATGCCTTGCCATCTACAGAGCAGG

 

TGTGACGG-3

A2: anti-S. typhimurium aptamer

5-CCAAAGGCTACGCGTTAACGTGGTGTTGG −3

R1: random sequence

5-ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCG

 

GGTACCCGGTTGAT-3

  1. The sequences of specific target bacteria binding aptamers [17] were truncated after prediction of the secondary structure using web-based Vienna RNA software.

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