Figure 6
Cell membrane integrity analysis via LDH leakage from cells. Assay carried out in SH-SY5Y and RAW 264.7 cells incubated with MNP-PEI and MNP-PEI-PEG at 0 (white bar), 1.56 (light grey bar), 3.125 (grey bar), 6.25 (dark grey bar), 12.5 (very dark grey bar) and 25 μg mL-1 (black bar) over a period of 72 h (n = 3 ± SE). A1 SH-SY5 cells incubated with MNP-PEI, A2 RAW 264.7 cells incubated with MNP-PEI, B1 SH-SY5 cells incubated MNP-PEI-PEG and B2 RAW 264.7 cells incubated with MNP-PEI-PEG. Results were calculated and compared to the positive control, whereby cells were lysed and 100% LDH release was assumed. Asterisk denotes significant increase compared with basal levels of LDH (p, 0.05).