Open Access

Structure of reverse microemulsion-templated metal hexacyanoferrate nanoparticles

  • Alberto Gutiérrez-Becerra1,
  • Maximiliano Barcena-Soto1,
  • Víctor Soto1,
  • Jesús Arellano-Ceja1,
  • Norberto Casillas1,
  • Sylvain Prévost2,
  • Laurence Noirez3,
  • Michael Gradzielski2 and
  • José I Escalante1Email author
Contributed equally
Nanoscale Research Letters20127:83

Received: 3 October 2011

Accepted: 20 January 2012

Published: 20 January 2012


The droplet phase of a reverse microemulsion formed by the surfactant cetyltrimethylammonium ferrocyanide was used as a matrix to synthesize nanoparticles of nickel hexacyanoferrate by adding just a solution of NiCl2 to the microemulsion media. Dynamic light scattering and small-angle neutron scattering measurements show that the reverse microemulsion droplets employed have a globular structure, with sizes that depend on water content. Transmission electron microscopy and electron diffraction are used to obtain information about the structure of the synthesized nanoparticles. The results show that the size and shape of the coordination compound nanoparticles correspond with the size and shape of the droplets, suggesting that the presented system constitutes an alternative method of the synthesis of metal hexacyanoferrate nanoparticles.


reverse micellestemplate methodnanoparticlesnickel hexacyanoferrate


Microemulsions represent thermodynamically stable liquid dispersions containing surfactant aggregates, which can often be found in a large region of the phase diagram of two- or multicomponent surfactant systems [1, 2]. They exhibit a well-defined structure that is characterized by a typical correlation length in the nanometer scale. Such microemulsions are of special interest because a variety of reactants can be introduced into the nanometer-sized aqueous domains, leading to materials with controlled size and shape [39]. Such characteristics play pivotal roles in controlling the physical, chemical, optical, and electronic properties of these nanomaterials. In the past few years, significant research has been conducted in the reverse microemulsion-mediated synthesis of inorganic (metal halides, selenides, or sulfides) and organic (cholesterol, rhodiarome, rhovanil, nimesulide, etc.) nanoparticles [313], and there has been substantial progress in the characterization of microemulsions of various types [14].

The synthesis of nanoparticles by reverse microemulsions is viable and attractive because it does not only produce nanoparticles that have a narrow size distribution, but also the particle size can be controlled by varying the microemulsion composition [15]. The reaction in a microemulsion may be conducted in two modes: (1) a multiple microemulsion method, where two or more microemulsions, each containing one reactant, are mixed together [16]. Upon mixing, the droplets collide with one another as a result of the Brownian motion. These collisions lead to the formation of product monomers [7, 17, 18]. Nucleation takes place in a given droplet when the number of product monomers exceeds the critical nucleation number [1921]. Further collisions between a droplet carrying a nucleus and another one carrying the product monomers cause the growth of the nucleus [19, 22]; (2) in the simple addition type, the reducing or precipitating reagent is directly added to the microemulsion containing the other reactant [23, 24], i.e., this mode promotes intramicellar nucleation and growth [22, 25]. When particles are formed in single microemulsions, their size and polydispersity are controlled by one or more of the following mechanisms: reaction kinetics, intramicellar nucleation and growth, intermicellar nucleation and growth, and particle aggregation [26, 27]. A variation of this synthetic path could proceed by replacing the counterion of the surfactant, and only then the addition of a salt to this reverse microemulsion media. This last method has been successfully used to synthesize nanoparticles using the anionic surfactant AOT [28], for instance, for the case of cobalt ferrocyanide salt nanoparticles [29]. However, to the best of our knowledge, there is no report on the modification of cationic surfactants with ionic coordination compounds such as the cetyltrimethylammonium ferrocyanide [CTAFeII].

Some advantages of this novel cationic surfactant are readily apparent; for instance, inverse microemulsion formed with this surfactant will allow synthesizing different transition metal hexacyanoferrates [Mhcf] by simply adding different salts to the microemulsion media, i.e., with the same surfactant, it is possible to produce different nanoparticles of coordination compounds (MIIhcf or MIIIhcf). Such compounds and other Prussian blue analogues have been a subject of several studies because of their promising characteristics which include electrochromism, the ability to mediate (electrocatalyze) redox reactions, ionic and electronic (mixed valence electron hopping) conductivities, capability for storage of countercations, and molecular magnetism [3032].

According to this motivation, in this paper, we studied the formation of a novel type of ferrocyanide-containing cationic surfactant and its ability to form reverse microemulsions. In this work, we use as surfactant a mixture of cetyltrimethylammonium bromide [CTAB] (95 wt.%) and CTAFeII (5 wt.%). The latter was prepared by replacing the bromide (Br-) ions of the cationic surfactant CTAB with ferrocyanide ([Fe(CN)6]4-) ions following a direct metathesis reaction in an aqueous phase [33]. This new surfactant, CTAFeII, presents a very limited area for a microemulsion phase, so when the mixture of surfactants was used, we reach a more extended region of the microemulsion. This can be explained taking into account the interfacial stiffness caused by the bulky molecules of CTAFeII (a huge counterion and four aliphatic chains). However, by adding CTAB molecules, it was possible to obtain an improvement in the interface flexibility. In addition, by changing the surfactant ratio of the mixture, it was found that the system offers better results to the nanoparticle synthesis when a low concentration of CTAFeII (5 wt.%) was used in the surfactant mixture. Furthermore, López-Quintela established that smaller nanoparticles can be obtained in microemulsions when there is a significant difference in the concentrations of the reactants [7].

Materials and methods


All the reactants used in this report were of analytical grade. CTAB was purchased from Sigma-Aldrich Corporation (99%; St. Louis, MO, USA), ferrocyanide salt (K4[Fe(CN)6]3H2O), from J.T. Baker (99%; Deventer, The Netherlands), n-hexane (C6H14) and NiCl26H2O, from Caledon Laboratories Ltd. (98% and 99%, respectively; Halton Hills, Canada), n-butanol (C4H9OH), from Productos Químicos Monterrey (99%; Monterrey, Nuevo León, Mexico), and double distilled water, from Selectropura S.A. de C.V. (σ = 1.5 to 3 μS/cm; Guadalajara, Jalisco, Mexico). For neutron scattering experiments, D2O (99.9% D; Euriso-Top, Gif-sur-Yvette, France) was used instead of H2O to increase the contrast and lower the background.

Synthesis of modified surfactant

The surfactant CTAFeII was prepared by a direct metathesis reaction in an aqueous phase. The detailed procedure is described in the study of Gutierrez-Bercerra et al. [33]. Functional groups of CTAB and CTAFeII were identified by a Fourier transform infrared [FTIR] spectrometer (Spectrum One, PerkinElmer, Waltham, MA, USA). Infrared spectra were recorded in the 400- to 4,000-cm-1 region, with a resolution of 4.00 cm-1.

Phase diagram

The pseudo-ternary phase diagram for the CTAB + CTAFeII + n-butanol/n-hexane/water system was constructed, considering as surfactant a mixture of CTAB, CTAFeII, and n-butanol, using W CTAB/W CTAFeII ratios of 0.95:0.05 and the (W CTAB + W CTAFeII)/W but ratio of 1, where W CTAB, W CTAFeII, and W but are the weights of CTAB, CTAFeII, and n- butanol, respectively. A simple titration technique was used to construct the diagram. Microemulsions were prepared by mixing weighed appropriate amounts of the individual components. The amount of n-hexane (W hex) in the surfactant mixture determines the H value (H = [W CTAB + W CTAFeII + W but]/[W hex + W CTAB + W CTAFeII + W but]), while W w = W water /(W water + W hex + W CTAB + W CTAFeII + W but) represents the weight fraction of water used as the titration component. Water was added in small volumes under permanent stirring in a tightly closed vial to avoid evaporation. Then, the vials were placed in a thermostatic bath (25°C) until a homogeneous media is reached.

Dynamic light scattering

Dynamic light scattering [DLS] measurements were performed using an ALV/CGS-3 goniometer with an ALV/LSE-5004 multiple tau digital correlator (ALV-Laser Vertriebsgesellschaft m-b.H., Langen, Germany). The light source was an He-Ne laser operating at a wavelength of 633 nm. The homodyne intensity autocorrelation function g (2)(τ) was measured at 90°. Data analysis was performed with the normalized intensity autocorrelation function using a third-order cumulant fit [34] that yielded as key parameter the effective collective diffusion coefficient.

Small-angle neutron scattering

Small-angle neutron scattering [SANS] measurements were done on the instrument PAXY at Laboratoire Léon Brillouin, Gif-sur-Yvette, France. A wavelength of 0.5 nm (FWHM 10%) was selected, and two configurations were used with sample-to-detector distances of 1.25 and 5.05 m.

Synthesis of nickel hexacyanoferrate

The synthesis of nickel hexacyanoferrate [Nihcf] nanoparticles was carried out at H = 0.4 and W w = 0.09. Appropriated amounts of CTAFeII, CTAB, n-butanol, and hexane were mixed until an H value of 0.4 was reached, and then it was maintained under stirring. After that, as an aqueous phase, a solution of 5 mM NiCl2 was added to the mixture to reach W w = 0.09. The microemulsion formed was stable for several days and at the same time maintaining a transparent state. Nihcf nanoparticles were separated from the microemulsion media by centrifugation at 9,000 rpm for 10 min. The precipitate was then washed several times with acetone. Despite the washing process, a small quantity of CTAB remained mixed with the nanoparticles. To obtain transmission electron microscopy [TEM] micrographs (JEM-1010, JEOL de Mexico S.A. de C.V., Mexico City, Mexico), a drop of the nanoparticles dispersed in acetone was placed directly on a carbon-coated copper grid. X-ray diffraction [XRD] patterns were recorded with a STOE Theta/theta X-ray diffractometer (STOE & Cie GmbH, Darmstadt, Germany) using a CuKα (λ = 0.15406 nm) at room temperature. FTIR spectra of the Nihcf were carried out in a PerkinElmer Spectrum One spectrometer.

Results and discussion

Surfactant characterization

For comparison, the IR spectra of the surfactants CTAB and CTAFeII are shown in Figure 1. The symmetric (υ s(CH2), d +) and asymmetric (υ as(CH2), d -) stretching vibrations of pure CTAB indicate equivalent gauche defects which lie at 2,849 and 2,918 cm-1 [35], as well as those of CTAFeII. The peaks at 3,017 and 1,487 cm-1, and at 1,473 and 1,462 cm-1 were attributed to the asymmetric and symmetric C-H scissoring vibrations of CH3-N+ moieties and to the CH2 scissoring mode, respectively [36]. The above mentioned results indicate that both surfactants possess a long aliphatic chain with a positively charged polar head as expected for the hydrocarbon CTAB structure. On the contrary, two peaks only appear in the CTAFeII: at 595 cm-1 due to the Fe-C vibration and at around 2,000 to 2,100 cm-1 due to the C≡N stretching [37]. Hence, it confirms that indeed the ferrocyanide ion is present in the CTAFeII. The low-spin Fe(II) is diamagnetic and will thus not have electronic transitions. The absorptions near 1,500 and between 1,550 and 1,700 cm-1 can be attributed to overtones and combination tones of OH- and H2O fundamental vibrations. The much lower reflectivity of the CTAFeII is a consequence of the high water content, which produces intense absorption with a broad band near 1,550 and 1,700 cm-1 because of the water present. In order to quantify this amount of water in CTAFeII samples, Karl Fisher titrations were carried out, obtaining a 2.8% in weight.
Figure 1

Surfactant characterization. Infrared spectra of CTAB (above) and CTAFeII (below).

Phase behavior

The pseudo-ternary phase diagram obtained for the CTAB + CTAFeII + n-butanol/n-hexane/water at 25°C is shown in Figure 2A. The boundary between the microemulsion and the non-microemulsion region was established by a systematic titration based on the clear-turbid observation and conductivity measurements (not shown here). This microemulsion region is transparent because of the small dispersion size of water droplets in the system. Outside this area, the mixture is turbid, indicating that the system reaches the solubilization boundary for water and forms big emulsion droplets. In order to study the influence of alcohol, in addition, a phase diagram with n- pentanol as cosurfactant was obtained (see Figure 2B). The reverse microemulsion region of both phase diagrams is reached at a similar composition but is more extended for the case of pentanol. Obviously, the effectiveness of the cosurfactant increases with increasing chain length, an effect that is typically observed for the formation of microemulsions induced by the addition of a cosurfactant [38, 39]. This indicates that the water solubility has no strong dependence on the type of alcohol and that similar conditions prevail at the amphiphilic interface of the microemulsion aggregates for butanol and pentanol. On the other side, by comparing the phase diagrams in Figure 2C, it is seen that the microemulsion region for the mixture of surfactants examined is much larger than the microemulsion region shown for CTAFeII. This shows that the mixture of surfactants favors larger solubilization of water compared to the pure CTAFeII surfactant.
Figure 2

Phase diagrams. Pseudo-ternary phase diagrams obtained at 25°C for the systems: (A) CTAB + CTAFeII/n-butanol/n-hexane/water, (B) CTAB + CTAFeII/alcohol/n-hexane/water, and (C) surfactant/n-butanol/n-hexane/water. The section closed within the solid (or dashed) lines corresponds to the microemulsion phase. The discontinued lines in (A) represent the H values used for the measurements. Symbols indicate the compositions for the SANS measurements.

Structural characterization

Figure 3 shows a representative plot of the correlation function g (2)(τ)-1 obtained for the microemulsions studied. The solid line is a fit to the data using the cumulant method [34]. For a reverse micellar solution, the third-order cumulant expansion of g (2)(τ)-1 varies linearly with 2q 2 τ. From the slope, the effective collective diffusion coefficients [D eff] were determined. As a first approximation to determine the droplet size, we considered that the microemulsion is formed by non-interacting droplets. In this condition, the hydrodynamic radius [R h] can be calculated by the Stokes-Einstein equation R h = KT/6πηD eff , where k is the Boltzmann constant, T, the temperature, and η, the solvent viscosity (the continuous phase in the case of microemulsions). The obtained radii (2.5 to 4.5 nm) are in the same range as those measured by SANS, proving that the non-interacting supposition can be applied in this system without significant error. D eff and R h depend on the H values (see inset in Figure 3, and Table 1), with larger droplets being present for smaller H. An explanation could be that by increasing the hexane content, less butanol is present at the amphiphilic interface (as it becomes dissolved in oil, whereas CTAB and CTAFeII should not be soluble in hexane to any significant extent). Thereby, the total amphiphilic interface available becomes smaller, which then explains the increase in size for a given amount of water. The R h is also proportional to the relative amount of water contained as demonstrated in earlier works [40, 41].
Figure 3

DLS curve and change of the diffusion coefficient with water content. Variation of the intensity correlation function g 2(τ)-1 with time for the microemulsion structure at W w = 0.056 and H = 0.4. Inset: D eff vs. W w for different H values, 0.4 (filled square) and 0.5 (empty circle).

Table 1

D eff and R h depend on the H values

W w

H= 0.4

H= 0.5


Deff × 10-10 (m2/s)

R h



Deff × 10-10 (m2/s)

R h






































W w, weight fraction of water; D eff, effective collective diffusion coefficient; R h, hydrodynamic radius of the droplets; and p, polydispersity obtained by DLS at 25°C for different H values.

SANS was applied for obtaining a more comprehensive structural picture in the relevant size range that, for our given q range, is about 0.5 to 15 nm, where q (q = 4 π sin (θ/2)/λ0, here, λ 0 and θ are the wavelength and the scattering angle, respectively) can be interpreted in terms of distances using d = 2 π /q. The obtained scattering curves as a function of q are given in Figure 4. At a low amount of water, the spectra have a low intensity. With increasing W w, the scattering intensity increases and a pronounced angular dependence develop. Apparently, the water core of the aggregates is now large enough to produce a noticeable scattering and is becoming bigger with increasing water content. The shape of the scattering curves at higher q already indicates that these reverse microemulsions have a globular structure.
Figure 4

SANS curves. SANS spectra (LLB) for reverse micelle solutions of CTAB + CTAFeII/n-butanol/n-hexane in deuterated water. (A) H = 0.4 at W w = 0.05 (filled circle) and 0.20 (empty circle); and H = 0.6 at W w = 0.05 (filled square) and 0.2 (empty square). (B) H = 0.5 at W w = 0.05 (empty square), 0.12 (empty circle), and 0.24 (empty triangle). Solid lines are the best fitting obtained by the model (see text).

In addition, a correlation peak is visible that becomes much more prominent with increasing water content in the reverse microemulsion, and at the same time, its maximum moves from 1.4 to 0.85 nm-1 (for a fixed H of 0.5). This, together with the intensity increase, shows that the aggregates grow substantially in size with increasing water content, where, however, it should be noted that in SANS basically, only the D2O core is visible as an aggregate due to the strong contrast between the two isotopes H and D.

The pronounced correlation peak has to be due to steric interactions between the reverse aggregates as electrostatic interactions in the oil-continuous medium should be negligible, but of course, at the concentrations employed, the volume fractions of the amphiphilic material (CTAB, CTAFeII, n-butanol) plus D2O are in the range of 34% to 59% v/v and therefore high enough to explain effective repulsion already on the basis of purely steric interactions. In addition, it is well known that in reverse microemulsions, the solvent oil molecules are to a certain extent bound to the reverse microemulsion aggregates [42, 43], thereby enhancing the effective volume fraction further.

The first analysis of the SANS data can be performed using the peak position q Peak to determine the number density 1 N of aggregates assuming simple cubic packing: 2π /q peak = 1N-1/3. Then, it can be recalled that 1 N can be expressed with the volume fraction Φ of the aggregated material and the volume of one aggregate V: N = Φ/V. Assuming spheres, a radius can be deduced. Depending on the choice of material to consider, either only D2O or the whole aggregated material (D2O, n-butanol, surfactant), two values are found that can be considered as values for the core (neglecting dissolved butanol) and for the entire droplet (neglecting the contribution from oil swelling the aliphatic chains of the surfactant); the core radius varies from 0.8 to 3.1 nm and is proportional to the water content of the microemulsion. The difference between the core and the overall radius is in all cases around 1.1 nm (1.03 to 1.25 nm), a reasonable value for the surfactant acting as a shell; taking into account the solvation of this shell by hexane, a higher value would be reached; using Tanford's length, the stretched C15 chain is 2.05 nm; the typically retained value of 75% to 80% of this elongation corresponds to lengths of 1.54 to 1.64 nm; the radius of the tetramethylammonium head group is 0.285 nm; the overall thickness expected for the swollen shell would then be 2.11 to 2.21 nm. However, notice that the R h, obtained by DLS and SANS, increases roughly linear with the water content as typically observed for reverse microemulsion droplets [4446].

Two-dimensional data were reduced using BerSANS accounting for dead time, transmission, and background scattering assimilated to the empty cuvette (which means that the incoherent scattering in the spectra still contains contributions from all compounds in the samples including the solvent), and the scattering from H2O in a 1-mm cuvette was used to account for the detector pixel efficiency and solid angle variations. Absolute scale was deduced from the evaluation of the direct beam flux. As all corrected scattering patterns are isotropic, they were finally radial-averaged, and data from two configurations were merged.

The whole scattering curves can be described by a model of globular aggregates interacting via an effective hard sphere potential for which the scattering intensity is given by:
I ( q ) = 1 N SLD p - SLD s 2 P ( q ) S ( q ) ,
where 1 N is the number density of particles, SLDp and SLDs, the scattering length densities of the particle and the solvent, respectively, P(q), the particle form factor assuming core-shell spheres, and S(q), the structure factor accounting for the interparticle interactions, keeping the same density number and using an adjustable hard sphere radius. The core is composed of D2O and butanol; the shell of polar charged moieties is composed of D2O, butanol, and counterions (bromide is known to adsorb strongly on alkylammonium interfaces, and the amount of ferrocyanide ions is negligible); and the matrix contains hexane and the aliphatic chains of the surfactants (Table 2). The shell thickness was fixed to the dimension of -CH2-N(CH3)3 + which is 0.57 nm [47]. The incompressibility of all the species was assumed as we do not have access to apparent molecular volumes in situ.
Table 2

Fit parameters for scattering curves



SLD × 10-9 (cm-2)










C15H31 -



CH2N(CH3)3 +






Fe(CN)6 -



SLD, scattering length densities and v, apparent molecular volumes employed in the fitting of the scattering curves.

To evaluate the feasibility of this model, where butanol is absent from the oil phase and partitions between the core and the shell, a comparison of the experimental invariants INV exp = 0 ( I ( q ) - I inc ) q 2 d q with the theoretical invariants
IN V th = 2 π 2 ϕ oil ϕ shell ( SL D oil - SL D shell ) 2 + ϕ oil ϕ core ( SL D oil - SL D core ) 2 + ϕ core ϕ shell ( SL D core - SL D shell ) 2
was performed with the partition coefficient of butanol between the core and the shell as the only adjustable parameter; the volume fraction of water in the shell was fixed to be identical to the volume of the tetraalkylammonium group (ca. four water molecules per group). Identity was found with the partition of butanol toward the water phase increasing with the amount of D2O, except in two cases where the amount of water was too little to actually allow for a core. The maximum volume fraction of butanol in the core does not exceed 16%, only slightly higher than the solubility limit of the alcohol in bulk water (9.5%). Accordingly, we think that our model is reasonable and self-consistent. In all cases, we observe a very good agreement between the values for the droplet core obtained by the analysis of the peak position and by the full fits (Table 3).
Table 3

Radius of the microemulsion droplets


W w

Volume fraction

q Peak


Rc (nm)







Rmic (nm)

Rc (nm)



K butanol






















































































R c and R mic, the core and the micelle radius, respectively, together with δ, shell thickness from the analysis of the peak position; R c, core radius from the fits; K butanol, butanol partitioning constant (for the distribution between the core and the shell); R h, hydrodynamic radius; W w, weight fraction of water; q Peak, peak position; Inv, invariant.

Synthesis of nanoparticles

The formation of Nihcf nanoparticles in this system was carried out in four principal stages as depicted in Figure 5. First, the dissociation of the surfactant counterions (ferrocyanide and bromide) is reached when the solution of NiCl2 is added (dropwise under vigorous stirring) to the reverse microemulsion. Then, the nickel and ferrocyanide ions react to form the first nuclei of Nihcf. Once the nuclei are formed, further growth of the particles is taking place via collisions with other microemulsion droplets containing additional salt precursors. Size and shape of nanoparticles are controlled by the steric stabilization provided by adsorbed surfactant molecules on the surface of the nanoparticles [48]. This prospective mechanism still has to be confirmed in more detail by further studies that are currently going on.
Figure 5

Mechanisms. Possible mechanism for the synthesis of Nihcf nanoparticles: (a) dissolution of all the ionic species, (b) formation reaction of nuclei of Nihcf, and (c, d) growth of nanoparticles due to the collisions between the droplets. To simplify, Cl- and Br- ions are not shown.

The reaction between [Fe(CN)6]4- ions and the CTAFeII and nickel(II) ions from the aqueous solution produces a colored (yellowish brown) microemulsion without precipitation, suggesting that this reaction is sufficiently facile to allow for the formation of Nihcf, while suppressing at the same time the further growth due to surfactant stabilization of the nanoparticles. Accordingly, the formed particles remain in the nanometer range and are colloidally dispersed (see Figure 6). The inset in this figure shows the indexing of the electron diffraction pattern of the sample with a [40] direction that coincides with the space group F43m characteristic of the Nihcf [49]. Figure 6 confirms the existence of small particles (approximately 6 nm in average size) which have a homogeneous size distribution and correspond to the droplet size of the initial microemulsion droplets (as measured by DLS and SANS).
Figure 6

TEM image of the nanoparticles. TEM image of the Nihcf nanoparticles synthesized. Inset: electron diffraction.

XRD and FTIR were performed in order to obtain a better characterization of the Nihcf nanoparticles. Figure 7A shows a comparison between the FTIR spectra of the stretching vibration of the cyano group in the Nihcf nanoparticles (solid line) and the surfactant CTAFeII (dashed line). The absorption band at 2,109 cm-1 can be assigned to the stretching vibration of the C≡N group into the CTAFeII. While for the Nihcf nanoparticles, this absorption band shifts to 2,096 cm-1 and represents the stretching vibration of the cyano group into the cyanoferrate lattice of Nihcf nanoparticles [Ni2+-C≡N-Fe2+] [50]. On the other hand, Figure 7B shows the XRD pattern of Nihcf nanoparticles mixed with CTAB remained from the washing process. In order to isolate the Nihcf nanoparticle contribution in the diffractogram, the peaks assigned to the diffraction of CTAB [51] were subtracted. The lattice parameter value for the nanoparticles calculated by indexing the peak position using an F43m lattice symmetry is 1.016 nm, close to the 1.000 nm reported elsewhere [49].
Figure 7

Nanoparticle characterization. (A) Comparison between the FTIR spectra of the stretching vibration of the cyano group in the Nihcf nanoparticles (solid line) and the surfactant CTAFeII (dashed line). (B) XRD patterns of the mixture Nihcf-CTAB; Nihcf nanoparticles are subtracted from the mixture pattern, and the peaks are assigned to CTAB diffraction according to Wong-Ng et al. [51].

An advantage identified in this work for the synthesis of coordination compound nanoparticles is that with this system, different transition Mhcf can be obtained only by varying the transition metal (copper(II), cobalt(II), iron(III), etc.) in the aqueous phase. This constitutes an alternative method using cationic, modified surfactants in reverse microemulsion for the synthesis of this type of nanoparticles.


In this work, the preparation of nanoparticles of transition Mhcf with a homogeneous size was performed using a simple process in which a droplet is regarded as a nanoreactor. Such soft technique provides good crystallinity in the absence of high temperature and pressure requirements, which favors the formation of small nanoparticles with controlled size and size distribution. Furthermore, it was found that the nanostructure of the particles obtained seems to be related to the structure of the template involved, namely the spherical water pool, at the conditions mentioned in this work.

The ratio of water to surfactant concentration plays an important role in determining the interaction of the water pool with the surfactant or bulk water. Hence, the size of the reverse microemulsion droplets increases as the water pool increases and vice versa. By varying the amount of water content, change in the size of the droplet formed is possible.

Furthermore, using a modified form of the surfactant CTAB (CTAFeII), it was possible to introduce a metal complex ion directly into a reverse microemulsion system without adding a salt as a further component. This procedure allows synthesizing, in a simple way, nanoparticles that correspond in size and shape to the microemulsion droplet morphology. In summary, these experiments demonstrate the feasibility of producing Nihcf nanoparticles using the surfactant CTAFeII.




This research was supported by the Mexican National Council of Science and Technology (CONACyT) and the Deutscher Akademischer Austauschdienst (DAAD, project: D/07/10253) through PROALMEX 2008-2010. The authors thank the CCMC-UNAM for performing the TEM experiments. The Laboratoire Léon Brillouin (LLB) of Saclay, France, is gratefully acknowledged for providing the SANS beamtime.

Authors’ Affiliations

Chemistry Department, University of Guadalajara, Guadalajara, Mexico
Stranski-Laboratorium für Physikalische und Theoretische Chemie, Institut für Chemie, Technische Universität Berlin, Berlin, Germany
Laboratoire Léon Brillouin (CEA-CNRS), CEA Saclay, Gif-sur-Yvette, France


  1. Langevin D: Micelles and microemulsions. Annu Rev Phys Chem 1992, 43: 341–369. 10.1146/annurev.pc.43.100192.002013View ArticleGoogle Scholar
  2. Olsson D, Wennerstroem H: Globular and bicontinuous phases of nonionic surfactant films. Adv Colloid Interface Sci 1994, 49: 113–146.View ArticleGoogle Scholar
  3. Osseo-Asare K: Microemulsion-mediated synthesis of nanosize oxide material. In Handbook of Microemulsion Science and Technology. Edited by: Kumar P, Mittal KL. New York: Marcel Dekker; 1999:549–603.Google Scholar
  4. Sager WFC: Controlled formation of nanoparticles from microemulsions. Curr Opin Colloid Interface Sci 1998, 3: 276–283. 10.1016/S1359-0294(98)80072-1View ArticleGoogle Scholar
  5. Ganguli AK, Ganguly A, Vaidya S: Microemulsion-based synthesis of nanocrystalline materials. Chem Soc Rev 2010, 39: 474. 10.1039/b814613fView ArticleGoogle Scholar
  6. Eastoe J, Hollamby MJ, Hudson L: Recent advances in nanoparticle synthesis with reversed micelles. Adv Colloid Interface Sci 2006, 128: 5–15.View ArticleGoogle Scholar
  7. López-Quintela MA: Synthesis of nanomaterials in microemulsions: formation mechanisms and growth control. Curr Opin Colloid Interface Sci 2003, 8: 37–144.View ArticleGoogle Scholar
  8. Solans C, Izquierdo P, Nolla J, Azemar N, Garcia-Celma MJ: Nanoemulsions. Curr Opin Colloid Interface Sci 2005, 10: 102–110. 10.1016/j.cocis.2005.06.004View ArticleGoogle Scholar
  9. Koetz J, Gawlitza K, Kosmella S: Formation of organically and inorganically passivated CdS nanoparticles in reverse microemulsions. Colloid Polym Sci 2010, 288: 257–263. 10.1007/s00396-009-2154-5View ArticleGoogle Scholar
  10. Li M, Mann S: Emergence of morphological complexity in BaSO4fibers synthesized in AOT micro-emulsions. Langmuir 2000, 16: 7088–7094. 10.1021/la0000668View ArticleGoogle Scholar
  11. Pileni MP: Mesostructured fluids in oil-rich regions: structural and templating approaches. Langmuir 2001, 17: 7476–7486. 10.1021/la010538yView ArticleGoogle Scholar
  12. Debuigne F, Cuisenaire J, Jeunieau L, Masereel B, Nagy JB: Synthesis of nimesulide nanoparticles in the microemulsion Epikuron/isopropyl myristate/water/ n -butanol (or isopropanol). J Colloid Interface Sci 2001, 243: 90–101. 10.1006/jcis.2001.7879View ArticleGoogle Scholar
  13. Margulis-Goshen K, Netivi HD, Major DT, Gradzielski M, Raviv U, Magdassi S: Formation of organic nanoparticles from volatile microemulsions. J Colloid Interface Sci 2010, 342: 283–292. 10.1016/j.jcis.2009.10.024View ArticleGoogle Scholar
  14. Gradzielski M: Recent developments in the characterisation of microemulsions. Curr Opin Colloid Interface Sci 2008, 13: 263–269. 10.1016/j.cocis.2007.10.006View ArticleGoogle Scholar
  15. Fernandez-Garcia M, Wang XQ, Belver C, Hanson JC, Rodriguez JA: Anatase TiO2nanomaterials: morphological/size dependence of the crystallization and phase behavior phenomena. J Phys Chem C 2007, 111: 674–682. 10.1021/jp065661iView ArticleGoogle Scholar
  16. Calandra P, Giordano C, Longo A, Turco-Liveri V: Physicochemical investigation of surfactant-coated gold nanoparticles synthesized in the confined space of dry reversed micelles. Mater Chem Phys 2006, 98: 494–499. 10.1016/j.matchemphys.2005.09.068View ArticleGoogle Scholar
  17. Holmberg K: Surfactant-templated nanomaterials synthesis. J Colloid Interface Sci 2004, 274: 355–364. 10.1016/j.jcis.2004.04.006View ArticleGoogle Scholar
  18. Aubert T, Grasset F, Mornet S, Duguet E, Cador O, Cordier S, Molard Y, Demange V, Mortier M, Haneda H: Functional silica nanoparticles synthesized by water-in-oil microemulsion processes. J Colloid Interface Sci 2010, 341: 201–208. 10.1016/j.jcis.2009.09.064View ArticleGoogle Scholar
  19. Bandyopadhaya R, Kumar R, Gandhi KS: Simulation of precipitation reactions in reverse micelles. Langmuir 2000, 16: 7139–7149. 10.1021/la000101aView ArticleGoogle Scholar
  20. Bandyopadhaya R, Kumar R, Gandhi KS, Ramkrishna D: Modeling of precipitation in reverse micellar systems. Langmuir 1997, 13: 3610–3620. 10.1021/la960599+View ArticleGoogle Scholar
  21. Schmidt J, Guesdon C, Schomäcker R: Engineering aspects of preparation of nanocrystalline particles in microemulsions. J Nanopart Res 1999, 1: 267–276. 10.1023/A:1010068903324View ArticleGoogle Scholar
  22. Natarajan U, Handique K, Mehra A, Bellare JR, Khilar KC: Ultrafine metal particle formation in reverse micellar systems: effects of intermicellar exchange on the formation of particles. Langmuir 1996, 12: 2670–2678. 10.1021/la940584gView ArticleGoogle Scholar
  23. Motte L, Billoudet F, Pileni MP: Synthesis in situ of nanosize silver sulphide semiconductor particles in reverse micelles. J Mater Sci 1996, 31: 38–42. 10.1007/BF00355123View ArticleGoogle Scholar
  24. Longo A, Calandra P, Casaletto MP, Giordano C, Venezia AM, Turco-Liveri V: Synthesis and physico-chemical characterization of gold nanoparticles softly coated by AOT. Mater Chem Phys 2006, 96: 66–72. 10.1016/j.matchemphys.2005.06.043View ArticleGoogle Scholar
  25. Pileni MP, Motte L, Petit C: Synthesis of cadmium sulfide in situ in reverse micelles: influence of the preparation modes on size, polydispersity, and photochemical reactions. Chem Mater 1992, 4: 338–345. 10.1021/cm00020a021View ArticleGoogle Scholar
  26. Husein MM, Rodil E, Vera JH: Preparation of AgBr nanoparticles in microemulsions via reaction of AgNO3with CTAB counterions. J Nanopart Res 2007, 9: 787–796. 10.1007/s11051-006-9107-4View ArticleGoogle Scholar
  27. Husein M, Rodil E, Vera JH: Formation of silver bromide precipitate of nanoparticles in a single microemulsion utilizing the surfactant counterion. J Colloid Interface Sci 2004, 273: 426–434. 10.1016/j.jcis.2004.02.057View ArticleGoogle Scholar
  28. Pileni MP: Nanosized particles made in colloidal assemblies. Langmuir 1997, 13: 3266–3276. 10.1021/la960319qView ArticleGoogle Scholar
  29. Giordano C, Longo A, Ruggirello A, Turco-Liveri V, Venezia A: Physicochemical investigation of cobalt-iron cyanide nanoparticles synthesized by a novel solid-solid reaction in confined space. Colloid Polym Sci 2004, 283: 265–276. 10.1007/s00396-004-1124-1View ArticleGoogle Scholar
  30. Itaya K, Uchida I, Neff VD: Electrochemistry of polynuclear transition metal cyanides: Prussian blue and its analogues. Acc Chem Res 1986, 19: 162–168. 10.1021/ar00126a001View ArticleGoogle Scholar
  31. Karyakin AA: Prussian blue and its analogues: electrochemistry and analytical applications. Electroanalysis 2001, 13: 813–819. 10.1002/1521-4109(200106)13:10<813::AID-ELAN813>3.0.CO;2-ZView ArticleGoogle Scholar
  32. Ricci F, Palleschi G: Sensor and biosensor preparation, optimisation and applications of Prussian Blue modified electrodes. Biosens Bioelectron 2005, 21: 389–407. 10.1016/j.bios.2004.12.001View ArticleGoogle Scholar
  33. Gutierrez-Bercerra A, Vega-Venegas T, Barcena-Soto M, Casillas N, Escalante JI: Obtaining NiHCF nanoparticles using a reverse micellar system. Materials Sci Forum 2010, 644: 47–50.View ArticleGoogle Scholar
  34. Frisken BJ: Revisiting the method of cumulants for the analysis of dynamic light-scattering data. Appl Op 2001, 40: 4087–4091. 10.1364/AO.40.004087View ArticleGoogle Scholar
  35. Liu XH, Luo XH, Lu SX, Zhang JC, Cao WL: A novel cetyltrimethyl ammonium silver bromide complex and silver bromide nanoparticles obtained by the surfactant counterion. J Colloid Interface Sci 2007, 307: 94–100. 10.1016/j.jcis.2006.11.051View ArticleGoogle Scholar
  36. Sui ZM, Chen X, Wang LY, Chai YC, Yang CJ, Zhao JK: An improved approach for synthesis of positively charged silver nanoparticles. Chem Lett 2005, 34: 100–101. 10.1246/cl.2005.100View ArticleGoogle Scholar
  37. Le Caër S, Vigneron G, Renault JP, Pommeret S: First coupling between a LINAC and FTIR spectroscopy: the aqueous ferrocyanide system. Chem Phys Lett 2006, 426: 71–76. 10.1016/j.cplett.2006.05.076View ArticleGoogle Scholar
  38. Mitra RK, Paul BK, Moulik SP: Phase behavior, interfacial composition and thermodynamic properties of mixed surfactant (CTAB and Brij-58) derived w/o microemulsions with 1-butanol and 1-pentanol as cosurfactants and n-heptane and n-decane as oils. J Colloid Interface Sci 2006, 300: 755–764. 10.1016/j.jcis.2006.04.011View ArticleGoogle Scholar
  39. Gradzielski M: Effect of the cosurfactant structure on the bending elasticity in nonionic oil-in-water microemulsions. Langmuir 1998, 14: 6037–6044. 10.1021/la980074cView ArticleGoogle Scholar
  40. Curri ML, Agostiano A, Manna L, Monica MD, Catalano M, Chiavarone L, Spagnolo V, Lugara M: Synthesis and characterization of CdS nanoclusters in a quaternary microemulsion: the role of the cosurfactant. J Phys Chem B 2000, 104: 8391–8397. 10.1021/jp0007639View ArticleGoogle Scholar
  41. Maidment LJ, Chen V, Warr GG: Effect of added cosurfactant on ternary microemulsion structure and dynamics. Colloids Surf A Physicochem Eng Aspects 1997, 129–130: 311–319.View ArticleGoogle Scholar
  42. Shaw DJ: Introduction to Colloid and Surface Chemistry. London: Butterworths; 1980.Google Scholar
  43. Kim MW, Dozier WD, Klein R: Light scattering measurements in a dilute microemulsion. J Chem Phys 1986, 84: 5919–5921. 10.1063/1.449904View ArticleGoogle Scholar
  44. Fletcher PDI, Rees GD, Robinson BH, Freedman RB: Kinetic properties of alpha-chymotrypsin in water-in-oil microemulsions: studies with a variety of substrates and microemulsion systems. Biochim Biophys Acta 1985, 832: 204–214. 10.1016/0167-4838(85)90333-4View ArticleGoogle Scholar
  45. Eicke HF, Borkovec M, Das-Gupta B: Conductivity of water-in-oil microemulsions: a quantitative charge fluctuation model. J Phys Chem 1989, 93: 314–317. 10.1021/j100338a062View ArticleGoogle Scholar
  46. Pileni MP: Reverse micelles as microreactors. J Phys Chem 1993, 97: 6961–6973. 10.1021/j100129a008View ArticleGoogle Scholar
  47. Conway BE, Verrall RE, Desnoyers JE: Partial molal volumes of tetraalkylammonium halides and assignment of individual ionic contributions. Trans Faraday Soc 1966, 62: 2738–2749.View ArticleGoogle Scholar
  48. Husein MM, Nassar NN: Nanoparticle preparation using the single microemulsions scheme. Current Nanoscience 2008, 4: 370–380. 10.2174/157341308786306116View ArticleGoogle Scholar
  49. Rigamonti R: Struttura e costituzione chimica di alcuni ferrocianuri. Gazz Chim Ital 1938, 68: 803–809.Google Scholar
  50. Chen W, Xia XH: Highly stable nickel hexacyanoferrate nanotubes for electrically switched ion exchange. Adv Funct Mater 2007, 17: 2943–2948. 10.1002/adfm.200700015View ArticleGoogle Scholar
  51. Wong-Ng W, McMurrie HF, Hubbard CR, Mighell AD: JCPDS-ICDD Research Associateship (cooperative program with NBS/NIST). J Res Natl Inst Stand Technol 2001, 106: 1013–1028.View ArticleGoogle Scholar


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