Figure 7

The mitochondrial functions of N9 cells affected by SWNHs, especially in pre-treated with LPS. Intact cellular basal OCR of N9 cells pre-treated with or without LPS induced by SWNHs measured by Seahorse XF24 analyzer. The OCR of N9 cells pre-treated with LPS (B) was more significant than N9 cells (A). Followed with the increasing concentrations of SWNHs, the OCR of N9 cells decreased significantly in a dose-dependent manner, especially in pre-treated with LPS (B) (P < 0.01). Steady state cellular ATP levels of N9 cells pre-treated with or without LPS induced by SWNHs were measured too. The steady state cellular alkaline phosphatase (APT) level of N9 cells pre-treated with LPS (D) was more significant than N9 cells (C). Followed with the increasing concentrations of SWNHs, the steady state cellular ATP level of N9 cells decreased significantly in a dose-dependent manner, especially in pre-treated with LPS (D) (P < 0.01). All data are represented as mean ± SEM.