LCSM analysis of ADS, 12DD, 21DD, and NC. The cells were treated with antibodies to membrane surface protein integrin β1 (red channel) (A1-D1). Nuclei were counterstained with DAPI (blue channel) (A2-D2). For each channel, the top view is presented. An overlay shows the two channels of each cell (A3-D3). A1-A3 showed ADS, B1-B3 showed 12DD, C1-C3 showed 21DD, and D1-D3 showed NC.