Skip to main content

Optimized gold nanoshell ensembles for biomedical applications


We theoretically study the properties of the optimal size distribution in the ensemble of hollow gold nanoshells (HGNs) that exhibits the best performance at in vivo biomedical applications. For the first time, to the best of our knowledge, we analyze the dependence of the optimal geometric means of the nanoshells’ thicknesses and core radii on the excitation wavelength and the type of human tissue, while assuming lognormal fit to the size distribution in a real HGN ensemble. Regardless of the tissue type, short-wavelength, near-infrared lasers are found to be the most effective in both absorption- and scattering-based applications. We derive approximate analytical expressions enabling one to readily estimate the parameters of optimal distribution for which an HGN ensemble exhibits the maximum efficiency of absorption or scattering inside a human tissue irradiated by a near-infrared laser.


The biocompatibility of gold nanoparticles, along with their tunable plasmon resonances and the ability to accumulate at targeted cancer sites, has proven them to be very effective agents for absorption-based photothermal therapy and scattering-based imaging applications [18]. Amongst the commonly used gold nanoparticles, silica-core gold nanoshells exhibit larger photothermal efficiency as compared to gold nanorods of equal number densities [1], whereas hollow gold nanoshells (HGNs) absorb light stronger than the silica-core gold nanoshells do [9, 10]. Furthermore, HGNs are comparatively less harmful to healthy tissues neighboring a cancer site [9], which makes them prospective for both photothermal and imaging applications. Although different tissue types and excitation wavelengths were analyzed before to determine the optimal dimensions of a nanoshell [10, 11], no optimization has ever been performed for a nanoshell ensemble with a real size distribution. In this Letter, we fill this gap by conducting the first theoretical study of the distribution parameters of the lognormally dispersed HGNs exhibiting peak absorption or scattering efficiency. In particular, we comprehensively analyze the dependence of these parameters on the excitation wavelength and optical properties of the tissue, giving clear design guidelines.


Despite a significant progress in nanofabrication technology over the past decade, we are still unable to synthesize large ensembles of almost identical nanoparticles. The nanoparticle ensembles that are currently used for biomedical applications exhibit broad size distributions, which are typically lognormal in shape [1215]. In an ensemble of single-core nanoshells, both the core radius R and the shell thickness H are distributed lognormally [15], with their occurrence probabilities given by the function [16]

f(x; μ X , σ X )= 1 x σ X 2 Π exp ln x μ X 2 2 σ X 2 ,

where x=r or h is the radius or thickness of the nanoshell, μ X = ln(Med[X]) and σ X are the mean and standard deviation of lnX, respectively, and Med[X] is the geometric mean of the random variable x=r or H.

The efficiencies of absorption and scattering by a nanoparticle ensemble are the key characteristics determining its performance in biomedical applications. In estimating these characteristics, it is common to use a number of simplifying assumptions. First of all, owing to a relatively large interparticle distance inside human tissue (typically constituting several micrometers [17]), one may safely neglect the nanoparticle interaction and the effects of multiple scattering at them [18, 19]. Since plasmonic nanoparticles can be excited resonantly with low-intensity optical sources, it is also reasonable to ignore the nonlinear effects and dipole–dipole interaction between biomolecules [20]. The absorption of the excitation light inside human tissue occurs on a typical length scale of several centimeters, within the near-infrared transparency window of 650 to 1000 nm [21]. However, the attenuation of light does not affect the efficiencies of scattering and absorption by the ensemble, and is therefore neglected in the following analysis. These simplifications allow us to relate the average absorption and scattering efficiencies (Sabs and Ssca) of the nanoshell ensemble embedded in a tissue to the corresponding efficiencies (Qabs and Qsca) of individual plasmonic nanoshells as

S α = Q α (r,h)f(r; μ R , σ R )f(h; μ H , σ H )drdh,

where Q α (r,h) is expressed through Mie coefficients for a coated sphere [9, 22, 23], which are the functions of the excitation wavelength, refractive index of the tissue, and permittivities of the nanoshell constituents.

It is seen that the average absorption and scattering efficiencies of a nanoshell ensemble, excited at a fixed wavelength, are functions of the four parameters: Med[R], Med[H], σ R , and σ H . This poses the problem of finding, and studying the properties of, the optimal distribution parameters for which the nanoshell ensemble exhibits the maximum absorption or scattering efficiency.

Results and discussions

We focus on HGNs with gold permittivity described by the size-dependent model from Ref. [9], and begin by evaluating their average absorption and scattering efficiencies inside a tissue of refractive index n=1.55. Figures 1(a) and 1(b) show these efficiencies in the parametric space of Med[R] and Med[H] for σ R =σ H =0.5 and excitation wavelength λ=850 nm. Each dependency is seen to exhibit a distinct peak in the form of a flat plateau, which arise predominantly due to the resonant interaction of light with the localized symmetric plasmon modes of the HGNs [9]. The absorption peaks for Med[R]≈44 nm and Med[H]≈9 nm, while the scattering reaches its maximum for larger and much thicker nanoshells, with Med[R]≈54 nm and Med[H]≈26 nm. The broadness of the peaks and the associated high tolerance of the nanoshell ensemble to the fabrication inaccuracies are the consequences of size distribution.

Figure 1
figure 1

Average (a) absorption and (b) scattering efficiencies of an hollow-gold-nanoshell ensemble with lognormal distribution. The ensemble is excited by monochromatic light at λ=850 nm. Optimal distributions of core radius and shell thickness for maximum [(c) and (d)] absorption and [(e) and (f)] scattering efficiencies of the ensemble excited at λ=750, 850, and 950 nm. In all cases, n=1.55 and σ R =σ H =0.5.

The effects of the excitation wavelength on the optimal distributions of the core radius and shell thickness are shown in Figures 1(c)– 1(f). Equal σ R and σ H (σ R =σ H =σ) correspond to the situation of similar (scalable) shapes of the two distributions. It is seen that the increase in the excitation wavelength shifts the optimal distribution f(r;μ R ,σ) towards larger radii for both absorption [Figure 1(c)] and scattering [Figure 1(e)]. This trend is opposite to the behavior of the optimal distributions f(h;μ H ,σ) in Figures 1(d) and 1(f), which shifts towards thinner shells with λ. Since the increase in Med[R] is larger than the reduction in Med[H], the optimal excitation of ensembles with larger HGNs require lower-frequency sources.

The optimal geometric means of HGNs’ dimensions crucially depend on the shape of size distribution determined by the parameter σ. Figure 2 shows how the optimal distributions of R and H are transformed when σ is increased from 0.1 to 1. As expected, larger σ results in broader distributions that maximize the absorption and scattering efficiencies of the nanoshell ensemble. It also leads to the right skewness of the distributions, thus increasing the fabrication tolerance. At the same time, the increase in σ from 0.1 to 1 reduces the peak values of Sabs and Ssca by about a factor of 3.5 each. This indicates the need of a compromise between the performance of an HGN ensemble and the fabrication tolerance. Regardless of σ, the ensemble exhibiting the maximum absorption efficiency comprises of HGNs with core radii smaller than those required for maximizing the scattering efficiency. A similar trend exists for the optimal distribution f(h;μ H ,σ), with absorbing nanoshells being much thinner than the scattering ones.

Figure 2
figure 2

Optimal lognormal distributions of core radius and shell thickness in an ensemble of hollow gold nanoshells exhibiting maximum average [(a) and (b)] absorption and [(c) and (d)] scattering efficiencies for σ R = σ H =0.1 , 0.25, 0.5, and 1.0. The simulation parameters are the same as in Figures 1(a) and 1(b).

The dependencies of the peak absorption and scattering efficiencies on the excitation wavelength are plotted in Figure 3(a) for n=1.55. The efficiencies are seen to monotonously decrease with λ, which makes shorter-wavelength near-infrared lasers preferable for both absorption- and scattering-based applications. Figures 3(b) and 3(c) show the dispersion of the geometric means for the optimal nanoshell distributions. One can see that the best performance is achieved for the nanoshells of smaller sizes, excited at shorter wavelengths. These results are summarized in the following polynomial fittings of the theoretical curves: Med[R]≈λ(21σ2−61σ+106)−44σ2+72σ−48 and Med[H]≈λ2(−58σ2+65σ+44)+λ(103σ2−127σ−78)−56σ2+77σ+39 for absorption, and Med[R]≈λ(281σ2−409σ+225)−266σ2+376σ−146 and Med[H]≈λ2(−966σ3+1921σ2−1150σ+244)+λ(1731σ3−3439σ2+2046σ−430)−803σ3+1607σ2−967σ+231for scattering. Here λ is expressed in micrometers, 0.1≤σ≤1, and the accuracy of the geometric means is about ±1 nm.

Figure 3
figure 3

[(a) and (d)] Optimal average absorption (filled circles) and scattering (open circles) efficiencies, and parameters [(b) and (e)] Med [R] and [(c) and (f)] Med[H] of the corresponding optimal distributions as functions of excitation wavelength and tissue refractive index. In (a)–(c), n=1.55; in (d)–(f), λ=850 nm. Solid, dashed, and dotted curves correspond to σ=0.25, 0.5, and 1.0, respectively.

The parameters of the optimal lognormal distribution also vary with the type of human tissue. Figures 3(d)–3(f) show such variation for the entire span of refractive indices of human cancerous tissue [9, 19], λ=850 nm, and three typical shapes of the distribution. It is seen that the peak efficiencies of absorption and scattering by an HGN ensemble grow with n regardless of the shape parameter σ. The corresponding geometric mean of the core radii reduces with n and may be approximated as Med[R]≈n(−51σ2+87σ−65)+72σ2−136σ+147 for absorption, and as Med[R]≈n(−94σ2+142σ−87)+114σ2−179σ+178 for scattering. In contrast, the optimal geometric mean of the shell thicknesses is almost independent of n and approximated by the polynomial Med[H]≈2σ2−3σ+10 in the case of absorption, and as Med[H]≈26σ2−41σ+40 in the case of scattering. These expressions allow estimation (with an accuracy of about ±1 nm) of the optimal distribution parameters of an HGN ensemble excited at λ=850 nm for 0.1≤σ≤1 and 1.35≤n≤1.7. Numerical calculations show that the optimal dependencies Med[R](n) and Med[H](n) have almost constant slopes for 650 nm≤λ≤1000 nm. This feature allows one to use Figure 3 to roughly estimate the optimal lognormal distributions of HGNs to be delivered to any human tissue illuminated by a near-infrared laser.


In summary, we have studied the optimal distributions of lognormally dispersed hollow gold nanoshells for different excitation wavelengths and human tissues. Shorter-wavelength, near-infrared sources were found to be most effective for in vivo biomedical applications. The analytical expressions obtained may be used to estimate the optimal distribution of the nanoshells providing the maximum efficiency of their absorption or scattering of near-infrared radiation inside human tissue.



Hollow Gold Nanoshell

Med[ X ] Median of the random variable X:



  1. Pattani VP, Tunnell JW: Nanoparticle-mediated photothermal therapy: A comparative study of heating for different particle types. Lasers Surg Med 2012, 44: 675—684.

    Article  Google Scholar 

  2. Akiyama Y, Mori T, Katayama Y, Niidome T: Conversion of rod-shaped gold nanoparticles to spherical forms and their effect on biodistribution in tumor-bearing mice. Nanoscale Res Lett 2012, 7: 565. 10.1186/1556-276X-7-565

    Article  Google Scholar 

  3. Kennedy LC, Bear AS, Young JK, Lewinski NA, Kim J, Foster AE, Drezek RA: T cells enhance gold nanoparticle delivery to tumors in vivo. Nanoscale Res Lett 2011, 6: 283. 10.1186/1556-276X-6-283

    Article  Google Scholar 

  4. Huang X, El-Sayed MA: Plasmonic photo-thermal therapy (PPTT). Alex J Med 2011, 47: 1–9. 10.1016/j.ajme.2011.01.001

    Article  Google Scholar 

  5. Liu L, Guo Z, Xu L, Xu R, Lu X: Facile purification of colloidal NIR-responsive gold nanorods using ions assisted self-assembly. Nanoscale Res Lett 2011, 6: 143. 10.1186/1556-276X-6-143

    Article  Google Scholar 

  6. Verma VC, Singh SK, Solanki R, Prakash S: Biofabrication of anisotropic gold nanotriangles using extract of endophytic Aspergillus clavatus as a dual functional reductant and stabilizer. Nanoscale Res Lett 2011, 6: 16.

    Article  Google Scholar 

  7. Chen Y, Hung Y, Liau I, Huang GS: Assessment of the in vivo toxicity of gold nanoparticles. Nanoscale Res Lett 2009, 4: 858–864. 10.1007/s11671-009-9334-6

    Article  Google Scholar 

  8. Jain PK, Lee KS, El-Sayed IH, El-Sayed MA: Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: Applications in biological imaging and biomedicine. J Phys Chem B 2006, 110: 7238–7248. 10.1021/jp057170o

    Article  Google Scholar 

  9. Sikdar D, Rukhlenko ID, Cheng W, Premaratne M: Effect of number density on optimal design of gold nanoshells for plasmonic photothermal therapy. Biom Opt Express 2013, 4: 15–31. 10.1364/BOE.4.000015

    Article  Google Scholar 

  10. Kessentini S, Barchiesi D: Quantitative comparison of optimized nanorods, nanoshells and hollow nanospheres for photothermal therapy. Biom Opt Express 2012, 3: 590–604. 10.1364/BOE.3.000590

    Article  Google Scholar 

  11. Grosges T, Barchiesi D, Kessentini S, Grehan G, de la Chapelle ML: Nanoshells for photothermal therapy: A Monte-Carlo based numerical study of their design tolerance. Biom Opt Express 2011, 2: 1584–1596. 10.1364/BOE.2.001584

    Article  Google Scholar 

  12. López-Muñoz GA, Pescador-Rojas JA, Ortega-Lopez J, Salazar JS, Balderas-López JA: Thermal diffusivity measurement of spherical gold nanofluids of different sizes/concentrations. Nanoscale Res Lett 2012, 7: 423. 10.1186/1556-276X-7-423

    Article  Google Scholar 

  13. Tengen TB: Designing nanomaterials with desired mechanical properties by constraining the evolution of their grain shapes. Nanoscale Res Lett 2011, 6: 585. 10.1186/1556-276X-6-585

    Article  Google Scholar 

  14. Amendola V, Meneghetti M: Size evaluation of gold nanoparticles by UV vis spectroscopy. J Phys Chem C 2009, 113: 4277–4285. 10.1021/jp8082425

    Article  Google Scholar 

  15. Wu G, Mikhailovsky A, A KH, A ZJ: Synthesis, characterization, and optical response of gold nanoshells used to trigger release from liposomes. Methods Enzymology 2009, 464: 279–307.

    Article  Google Scholar 

  16. Crow EL, Shimizu K: Lognormal distributions: Theory and applications. New York: M. Dekker; 1988.

    Google Scholar 

  17. Kah JCY, Chow TH, Ng BK, Razul SG, Olivo M, Sheppard CJR: Concentration dependence of gold nanoshells on the enhancement of optical coherence tomography images: a quantitative study. Appl Opt 2009, 48: D96-D108. 10.1364/AO.48.000D96

    Article  Google Scholar 

  18. Handapangoda CC, Premaratne M, Paganin DM, Hendahewa PRDS: Technique for handling wave propagation specific effects in biological tissue: Mapping of the photon transport equation to maxwell’s equations. Opt Express 2008, 16: 17792–17807. 10.1364/OE.16.017792

    Article  Google Scholar 

  19. Vo-Dinh T: Biomedical Photonics Handbook. CRC, Boca Raton: Florida; 2003.

    Book  Google Scholar 

  20. Rubinov AN, Afanas’ev AA: Nonresonance mechanisms of biological effects of coherent and incoherent light. Opt Spectrosc 2005, 98: 943–948. 10.1134/1.1953991

    Article  Google Scholar 

  21. Yu G: Near-infrared diffuse correlation spectroscopy in cancer diagnosis and therapy monitoring. J Biom Opt 2012, 17: 010901–010911. 10.1117/1.JBO.17.1.010901

    Article  Google Scholar 

  22. Tang Y, Vlahovic B: Metallic nano-particles for trapping light. Nanoscale Res Lett 2013, 8: 65. 10.1186/1556-276X-8-65

    Article  Google Scholar 

  23. Bohren CF, Huffman DR: Absorption and scattering of light by small particles. New York: Wiley; 1998.

    Book  Google Scholar 

Download references


The work of D. Sikdar is supported by the Department of Business and Innovation of the Victorian Government, through its Victoria India Doctoral Scholarship Program (managed by the Australia India Institute). The work of I. D. Rukhlenko and M. Premaratne is supported by the Australian Research Council, through its Discovery Early Career Researcher Award DE120100055 and Discovery Grant scheme under Grant DP110100713, respectively. The work of W. Cheng is supported the Australian Research Council, through its Discovery Grant scheme under Grant DP120100170.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Ivan D Rukhlenko.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

IDR, WC, and MP jointly suggested the study conducted by DS. DS conceived the model, performed the simulations, and prepared the manuscript. IDR, WC, and MP supervised the study, participated in the analysis of the results, helped DS to interpret and present the obtained result, and thoroughly edited the manuscript. All authors read and approved the final manuscript.

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Authors’ original file for figure 2

Authors’ original file for figure 3

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Sikdar, D., Rukhlenko, I.D., Cheng, W. et al. Optimized gold nanoshell ensembles for biomedical applications. Nanoscale Res Lett 8, 142 (2013).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • Hollow gold nanoshells
  • Lognormal distribution
  • Absorption
  • Scattering
  • Biomedical applications