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Figure 3 | Nanoscale Research Letters

Figure 3

From: Synapsable quadruplex-mediated fibers

Figure 3

Native gel electrophoresis images showing that quadruplex is present in synapsed (SQ1A:SQ1B) 2 . TMACl (top row): Samples in lanes 2, 4, and 6 contain 1.0 × 10−5 mol/L (10 μM) NMM. Lanes 1 and 2, 4.0 × 10−5 mol/L (40 μM) SQ1A:SQ1B duplex; lanes 3 and 4, mixture of 4.0 × 10−5 mol/L (40 μM) C1A:C1B duplex with 1.0 × 10−4 (100 μM) C1A; lanes 5 and 6, 8.0 × 10−5 mol/L (80 μM) per strand SQ1A. Gel (acrylamide mass fraction 12%) was run in 0.01 TMgTB buffer and (a) UV-shadowed (b) or UV-transilluminated. KCl (bottom row): All samples contain 1.0 × 10−5 mol/L (10 μM) NMM. Lane 1, 4.0 × 10−5 mol/L (40 μM) C1A:C1B duplex; lane 2, 4.0 × 10−5 mol/L (40 μM) SQ1A:SQ1B duplex in TMACl; lane 3, 3.0 × 10−5 mol/L (30 μM) SQ1A:SQ1B duplex incubated overnight at 4°C in high potassium-containing buffer to assemble quadruplex; lane 4, 6.0 × 10−5 mol/L (60 μM) per strand SQ1A. Gel (acrylamide mass fraction 12%) was run in 0.01 KMgTB buffer and (c) UV-shadowed or (d) UV-transilluminated.

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