- Nano Express
- Open Access
Luminescent properties of CdTe quantum dots synthesized using 3-mercaptopropionic acid reduction of tellurium dioxide directly
Nanoscale Research Letters volume 8, Article number: 253 (2013)
A facile one-step synthesis of CdTe quantum dots (QDs) in aqueous solution by atmospheric microwave reactor has been developed using 3-mercaptopropionic acid reduction of TeO2 directly. The obtained CdTe QDs were characterized by ultraviolet–visible spectroscopy, fluorescent spectroscopy, X-ray powder diffraction, multifunctional imaging electron spectrometer (XPS), and high-resolution transmission electron microscopy. Green- to red-emitting CdTe QDs with a maximum photoluminescence quantum yield of 56.68% were obtained.
In recent years, water-soluble CdTe luminescent quantum dots (QDs) have been used in various medical and biological imaging applications because their optical properties are considered to be superior to those of organic dyes [1–4]. Up to now, in most of the aqueous approaches, Te powder was used as the tellurium source and NaBH4 as the reductant, which needs a pretreatment to synthesize the unstable tellurium precursor. The process of preparing CdTe QDs requires N2 as the protective gas at the initial stage [5–10]. Even though Na2TeO3 as an alternative tellurium source can also be used for preparing CdTe QDs [11–15], it is toxic and expensive. Therefore, it is very necessary to hunt for a novel tellurium source for the synthesis of CdTe QDs. Compared with Na2TeO3, TeO2 has the same oxidation state of Te and is stable, cheap, and less toxic. Recently, TeO2 was explored as the Te source for synthesis of CdTe QDs, but the reduction of TeO2 by NaBH4 in ambient conditions requires a long reaction time and easily produces a black precipitate of CdTeO3[16–20]. Here, we proposed a new facile synthetic approach for preparing CdTe QDs with tellurium dioxide as a tellurium source. 3-mercaptopropionic acid was explored as both reductant for the reduction of TeO2 and capping ligand for CdTe QDs. Such synthetic approach eliminates the use of NaBH4 and allows facile one-pot synthesis of CdTe QDs.
Tellurium dioxide (TeO2, 99.99%), cadmium chloride hemi(pentahydrate) (CdCl2 · 2.5H2O, 99%), and 3-mercaptopropionic acid (MPA, 99%) were purchased from Aldrich Corporation (MO, USA). All chemicals were used without additional purification. All the solutions were prepared with water purified by a Milli-Q system (Millipore, Bedford, MA, USA).
Synthesis of CdTe QDs
In our experiments, 2 mmol CdCl2 · 2.5H2O was dissolved in 100 mL of deionized water in a breaker, and 5.4 mmol MPA was added under stirring. The pH of the solution was then adjusted to 10.0 by dropwise addition of 1 mol/L NaOH solution. Under stirring, 0.5 mmol TeO2 was added to the original solution. The typical molar ratio of Cd2+/Te2−/MPA was 1:0.25:2.7. The monomer was heated in a XO-SM100 microwave-assisted heating system (XO-SM100 Microwave and Ultrasonic combination response system, MW-50%; Xianou Company, Nanjing, China) and refluxed at different times to control the size of the CdTe QDs. The particles were extracted by precipitation with the addition of 2-propanol to the solution. Then, the resulting powders were dried at room temperature.
The absorption and photoluminescence (PL) spectra were measured using a UV-2501PC spectrometer (Shimadzu Corporation, Tokyo, Japan) and CARY ECLIPSE (Agilent Technologies, Santa Clara, USA) fluorescence spectrometer, respectively. The PL quantum yield was determined using Rhodamine 6G as fluorescence standard. X-ray powder diffraction (XRD) analysis was performed using a Dmax-2500 (CuKα = 1.5406 Å; Rigaku Corporation, Tokyo). The morphology of the QDs was characterized using JEM-2100 transmission electron microscopy (HR-TEM; Jeol Ltd., Tokyo). X-ray photoelectron spectra (XPS) were recorded by Thermo ESCALAB 250XI X-ray photoelectron spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) with nonmonochromatized Al Kα radiation as excitation source.
Results and discussion
The typical absorption PL spectra of CdTe QDs obtained with different refluxing times were given in Figure 1a. The redshifts of the absorption edge and the maximum PL emission wavelength indicated the growth of CdTe QDs during the heating treatment. The sizes of the QDs could be estimated from the UV–vis absorption spectrum by Yu and colleagues' empirical equation :
where λ is the first absorption maximum. The diameters of the QDs ranged from 2.27 to 3.44 nm, indicating that the size of the QDs could be facilely tuned by varying the heating time. The fluorescent color under UV irradiation changed from green to yellow, orange, and finally to red with increasing heating time (Figure 1b).
The PL quantum yield also depended on heating time (Figure 2). Increasing the heating time led to increased PL quantum yield, and maxima occurred at 120 min. Such PL quantum yield increase could be ascribed to the improvement of the crystallization and annealing effect of defects. However, further heating resulted in a decrease in PL quantum yield due to broad distribution and relatively small surface/volume ratio of the obtained QDs. Another evidence of the broad distribution is the increased full width at half maximum (FWHM) of the resultant CdTe QDs, which broadened from 40 to 66 nm in the heating time of 0 to 270 min. With heating time longer than 300 min, there were lots of black depositions in the solution, which may be caused by the oxidization and aggregation of CdTe QDs due to the destruction of MPA. Meanwhile, the PL quantum yield of the CdTe QDs decreases dramatically.
The as-prepared CdTe QDs were further characterized with XRD, TEM, HR-TEM, and XPS. As shown in Figure 3a, the diameter of the as-prepared CdTe QDs (refluxed for 120 min) is about 3 nm, which is very close to that estimated from Yu and colleagues' empirical equation . Typical HR-TEM image in Figure 3b indicated good crystalline structure of the CdTe QDs. The XRD pattern of CdTe QDs (Figure 3c) shows three diffraction peaks at 24.5°, 40.6°, and 48°, which can be readily assigned to the (111), (220), and (311) planes. Such characteristic diffraction pattern is the sign of the typical zinc-blend structure (JCPDS No. 65–1046).
Figure 4 shows the corresponding elemental composition by recording XPS core level spectra. Figure 4a shows an overview spectrum of the CdTe QDs. Different Cd and Te core levels can be seen. Furthermore, the main source of carbon, oxygen, and sulfur elements was from the stabilizer MPA. In our study, we focused on the Cd 3d, Te 3d, and S 2p levels. The Cd 4d and Te 4d levels have not been studied here because they are quite close to the valence band and, therefore, less reliable to analyze. The spectra of the Cd 3d and Te 3d level have been recorded in Figure 4b,c. The appearances of Cd 3d3/2 peak at 411.9 eV, Cd 3d5/2 peak at 405.2 eV, Te 3d5/2 peak at 572.5 eV, and Te 3d3/2 peak at 582.8 eV confirm the existence of cadmium and tellurium species in the CdTe QDs. This is in agreement with the previous reports  and further confirms the formation of CdTe QDs. Moreover, it can be seen clearly in the figure that two additional peaks appeared at binding energies of 576.0 and 586.6 eV, corresponding to the Te-O bonding states in CdTeO3, which are possible products from the oxidation reactions of CdTe QDs . As mentioned in the experimental section, the CdTe QDs are capped with MPA. So, the study of the S 2p level is in principle of interest, especially because some sulfur atoms originating from the organic stabilizer may have been incorporated into the CdTe lattice during the synthesis [24, 25]. Figure 4d shows the S 2p spectrum of the CdTe QDs. The S 2p core level spectrum shows a single signal, where the S 2p3/2 peak appears at 162.3 eV; this may suggest that there was no sulfur incorporated into the CdTe lattice because the S 2p3/2 level in CdS has a binding energy of 161.7eV .
Selenite (SeO32−) has long been known to react with thiols [27, 28], we suggest that the tellurium precursor reacts in a similar manner to the selenium analogue. In this work, we explored TeO2 as the Te source and MPA as both the reductant for TeO2 and capping ligand for CdTe QDs. It has been reported that tellurite could be reduced to H2Te by glutathione via the GS-Te-SG complex . We proposed that TeO2 could also be reduced to Te2− in the presence of MPA as follows:
In strong alkali solutions, TeO2 was firstly dissolved and formed TeO32- anion. Meanwhile, Cd2+ is complexed by RSH (MPA) and forms Cd(RS)+. In the presence of excess MPA, tellurite is first slowly formed to RS-Te-SR (3), and then the RS-Te-SR is further reduced by MPA into RS-TeH/RS-Te− (4) and H2Te/HTe−/Te2− (5). The CdTe QDs were obtained by the reaction between HTe− and Cd2+ in the presence of MPA, according to reaction (6).
The generation of Te2− was further verified via a control experiment. As shown in Figure 5, in the absence of MPA, tellurite solution is colorless and transparent. Soon after the injection of MPA, the solution color changed to pale yellow immediately, an indication of the formation of HTe−. In open air condition, the solution color further changed to brown and black in about 7 min. In addition, lots of black Te precipitation was observed in the bottom of the solution due to the oxidation of Te2− in open air.
We further compared the use of MPA and NaBH4 as reductant for synthesis of CdTe QDs. As shown in Figure 6, using MPA as reductant for TeO32− resulted in CdTe QDs with stronger fluorescence intensity and longer emission wavelength, in comparison with those synthesized with NaBH4 as the reductant. NaBH4 is a more powerful reductant than MPA for TeO32−. Accordingly, much more Te2− ions could be generated, and more CdTe nuclei for subsequent growth of QDs. At a higher precursor concentration, more nuclei were formed, and these nuclei quickly expanded the remaining monomers with the growth of nuclei. Thus, the few remaining Cd monomers probably caused the ineffective passivation of nanocrystal surface defects, which induced the weak luminescence. However, at a lower precursor concentration, fewer nuclei were formed and relatively more cadmium monomers remained in the solution, which were favorable for surface ordering and reconstructions of CdTe QDs during the growth of QDs.
In summary, a facile synthetic route for the preparation of water-soluble CdTe QDs has been proposed using 3-mercaptopropionic acid reduction of TeO2 directly. Since the raw materials are cheap and easy to be obtained, the synthesis process is simple, fast, and mild. The as-synthesized CdTe QDs were highly luminescent, which ensures its promising future applications as biological labels.
Sandra JR, Jerry CC, Oleg K, McBride JR, Tomlinson ID: Biocompatible quantum dots for biological applications. Chem Biol 2011, 18(1):10–24. 10.1016/j.chembiol.2010.11.013
Zhang G, Shi L, Selke M, Wang XM: CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation. Nanoscale Res Lett 2011, 6(1):418–423. 10.1186/1556-276X-6-418
Ge S, Zhang C, Zhu Y, Yu J, Zhang S: BSA activated CdTe quantum dot nanosensor for antimony ion detection. Analyst 2010, 135(1):111–115. 10.1039/b915622d
Wu P, Yan X: Doped quantum dots for chemo/biosensing and bioimaging. Chem Soc Rev 2013. in press in press
Wang JF, Song XT, Li L, Qian HF, Chen KY, Xu XM, Cao CX, Ren JC: Exploring feasibility for application of luminescent CdTe quantum dots prepared in aqueous phase to live cell imaging. Chin Chem Lett 2006, 17(5):675–678.
Li L, Qian H, Ren J: Rapid synthesis of highly luminescent CdTe nanocrystals in the aqueous phase by microwave irradiation with controllable temperature. Chem Commun 2005, 4: 528–530.
Ghosh S, Saha A: Synthesis and spectral studies of CdTe–dendrimer conjugates. Nanoscale Res Lett 2009, 4: 937–941. 10.1007/s11671-009-9339-1
Yuan J, Guo W, Yin J, Wang E: Glutathione-capped CdTe quantum dots for the sensitive detection of glucose. Talanta 2009, 77(5):1858–1863. 10.1016/j.talanta.2008.10.032
Silva FO, Carvalho MS, Mendonca R, Macedo WA, Balzuweit K: Effect of surface ligands on the optical properties of aqueous soluble CdTe quantum dots. Nanoscale Res Lett 2012, 7: 536–545. 10.1186/1556-276X-7-536
Sai LM, Kong XY: Microwave-assisted synthesis of water-dispersed CdTe/CdSe core/shell type II quantum dots. Nanoscale Res Lett 2011, 6: 399–405. 10.1186/1556-276X-6-399
Zhou D, Lin M, Chen ZL, Sun HZ, Zhang H, Sun HC, Yang B: Simple synthesis of highly luminescent water-soluble CdTe quantum dots with controllable surface functionality. Chem Mater 2011, 23(21):4857–4862. 10.1021/cm202368w
Ge CW, Xu M, Liu J, Lei JP, Ju HX: Facile synthesis and application of highly luminescent CdTe quantum dots with an electrogenerated precursor. Chem Commun 2008, 4: 450–452.
Bao HF, Wang EK, Dong SJ: One-pot synthesis of CdTe nanocrystals and shape control of luminescent CdTe–cystine nanocomposites. Small 2006, 2: 476–480. 10.1002/smll.200500346
Ying E, Li D, Guo SJ, Dong S, Wang J: Synthesis and bio-imaging application of highly luminescent mercaptosuccinic acid-coated CdTe nanocrystals. J PLoS One 2008, 3: e2222. 10.1371/journal.pone.0002222
Sheng ZH, Han HY, Hu XF, Chi C: One-step growth of high luminescene CdTe quantum dots with low cytotoxicity in ambient atmospheric conditions. Dalton Trans 2010, 39(30):7017–7020. 10.1039/c002618b
Wu P, Yan XP: Ni2+-modulated homocysteine-capped CdTe quantum dots as a turn-on photoluminescent sensor for detecting histidine in biological fluids. Biosens Bioelectron 2010, 26: 485–490. 10.1016/j.bios.2010.07.068
Wang YY, Cai KF, Yin JL, Yao X: Facile synthesis and photoluminescence properties of water-soluble CdTe/CdS core/shell quantum dots. Micro Nano Lett 2011, 6: 141–143. 10.1049/mnl.2011.0012
Wang RF, Wang YL, Feng QL, Zhou LY, Gong FZ, Lan YW: Synthesis and characterization of cysteamine-CdTe quantum dots via one-step aqueous method. Mater Lett 2012, 66: 261–263. 10.1016/j.matlet.2011.08.096
Wang YL, Liu SY, Zhou LY: An alternative aqueous synthetic route to preparing CdTe quantum dots with tunable photoluminescence. Chinese Chem Lett 2012, 23: 359–362. 10.1016/j.cclet.2012.01.012
Shen HB, Wang HZ, Chen X, Niu JZ, Xu WW, Li XM, Jiang XD, Du ZL, Li LS: Size- and shape-controlled synthesis of CdTe and PbTe nanocrystals using tellurium dioxide as the tellurium precursor. Chem Mater 2010, 22: 4756–4761. 10.1021/cm1013009
Yu WW, Qu LH, Guo WZ, Peng XG: Experimental determination of the extinction coefficient of CdTe, CdSe and CdS nanocrystals. Chem Mater 2003, 15: 2854–2860. 10.1021/cm034081k
Zhang H, Zhou Z, Yang B, Gao MY: The influence of carboxyl groups on the photoluminescence of mercaptocarboxylic acid-stabilized CdTe nanoparticles. J Phys Chem B 2003, 107(1):8–13. 10.1021/jp025910c
Zhang Y, He J, Wang PN, Chen JY, Lu ZJ, Lu DR, Guo J, Wang CC, Yang WL: Time-dependent photoluminescence blue shift of the quantum dots in living cells: effect of oxidation by singlet oxygen. J Am Chem Soc 2006, 128: 13396–13401. 10.1021/ja061225y
Gaponik N, Talapin DV, Rogach AL, Hoppe K, Shevchenko EV, Kornowski A, Eychmüller A, Weller H: Thiol-capping of CdTe nanocrystals: an alternative to organometallic synthetic routes. J Phy Chem B 2002, 106(29):7177–7185. 10.1021/jp025541k
Rogach AL: Nanocrystalline CdTe and CdTe(S) particles: wet chemical preparation, size-dependent optical properties and perspectives of optoelectronic applications. Mater Sci Eng B 2000, 69–70: 435–440.
Borchert H, Talapin DV, Gaponik N, McGinley C, Adam S, Lobo A, Möller T, Weller H: Relations between the photoluminescence efficiency of CdTe nanocrystals and their surface properties revealed by synchrotron XPS. J Phys Chem B 2003, 107: 9662–9668. 10.1021/jp0352884
Tarze A, Dauplais M, Grigoras L, Lazard M, Barbier F, Blanquet S, Plateau P: Extracellular production of hydrogen selenide accounts for thiol-assisted toxicity of selenite against Saccharomyces cerevisiae . J Bio Chem 2007, 282: 8759–8767. 10.1074/jbc.M610078200
Cui R, Gu YP, Zhang ZL, Xie ZX, Tian ZQ, Pang DW: Controllable synthesis of PbSe nanocubes in aqueous phase using a quasi-biosystem. J Mater Chem 2012, 22: 3713–3716. 10.1039/c2jm15691a
Stürzenbaum SR, Höckner M, Panneerselvam A, Levitt J, Bouillard JS, Taniguchi S, Dailey LA, Khanbeigi RA, Rosca EV, Thanou M, Suhling K, Zayats AV, Green M: Biosynthesis of luminescent quantum dots in an earthworm. Nat Nanotechnol 2013, 8: 57–60.
The authors gratefully acknowledge the support for this research from Zhejiang Provincial Natural Science Foundation of China under grant no. LQ12B03002 and from the National Natural Science Foundation of China under grant no. 21207095, as well as the State Key Laboratory of Chemical Resources Engineering under grant no. CRE-2012-C-303.
The authors declare that they have no competing interests.
MS carried out the total experiment and wrote the manuscript. WJ participated in the data analysis. YH, YJ, and DH supervised the project. FL, ST, and JL provided the facilities and discussions related to them. YJ participated in the detection of the XPS and TEM. All authors read and approved the final manuscript.
Authors’ original submitted files for images
About this article
Cite this article
Shen, M., Jia, W., You, Y. et al. Luminescent properties of CdTe quantum dots synthesized using 3-mercaptopropionic acid reduction of tellurium dioxide directly. Nanoscale Res Lett 8, 253 (2013). https://doi.org/10.1186/1556-276X-8-253
- CdTe quantum dots
- 3-mercaptopropionic acid
- Microwave irradiation