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Table 2 Physico-chemical properties of PBH-capped AuNPs (100 μg/ml) under different conditions over time

From: Peptide-biphenyl hybrid-capped AuNPs: stability and biocompatibility under cell culture conditions

	Milli-Q water			EMEM/S+		EMEM/S-
	T0	T24	T0	T0	T24	T0	T24
AuNP	Size ^a	Size	Zeta ^b	Size	Size	Size	Size
AuNP	nm	nm	mV	nm	nm	nm	nm
Au[(Gly-Trp-Met)₂B]	148 ± 2	148 ± 1	−31.6 ± 2.0	242 ± 4	243 ± 6	233 ± 15	1,239 ± 26
Au[(Gly-Tyr-TrCys) ₂ B]	143 ± 1	143 ± 1	−37 ± 1.4	261 ± 1	261 ± 2	251 ± 15	195 ± 2
Au[(Gly-Tyr-Met)₂B]	591 ± 73	507 ± 65	−36 ± 1.1	987 ± 205	987 ± 207	407 ± 21	1,230 ± 8
Au[(Gly-Tyr-Met)₂B]	161 ± 5	150 ± 12		203 ± 13	201 ± 9
Au[(Met)₂B]	229 ± 23	228 ± 10	−39 ± 1.1	190 ± 13	190 ± 4	1568 ± 28	1,368 ± 25
Au[(Met)₂B]	38 ± 6	40 ± 3		27 ± 9	28 ± 3
Au[(TrCys)₂B]	205 ± 1	205 ± 1	−43.2 ± 1.1	261 ± 3	260 ± 4	271 ± 23	908 ± 23
							97 ± 3

T0 represents measurements directly after preparation and T24 measurements 24 h after incubation under cell exposure conditions (37°C, 5% CO₂). Average values of three independent measurements are presented (mean ± SD). Bold emphasis is used to signal the most stable AuNP; DLS, dynamic light scattering. ^aHydrodynamic size (Size); ^bzeta potential (Zeta) of AuNPs in Milli-Q water.

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