Peptide-biphenyl hybrid-capped AuNPs: stability and biocompatibility under cell culture conditions

Table 3 Cytotoxicity of PBH-capped AuNPs following 24- and 48-h exposure (EMEM/S-), using resazurin assay

		Exposure concentration (μg/ml)
Exposure duration	AuNP	12.5	25	50	100
Au[(Gly-Trp-Met)₂B]	24 h	97 ± 1	97 ± 1*	96 ± 1*	94 ± 0.3**^a
Viability (%)	48 h	98 ± 1	98 ± 2	91 ± 1	69 ± 4**^a
Measured interference (%)		96 ± 2	95 ± 2	94 ± 4	88 ± 4
Au[(Gly-Tyr-TrCys) ₂ B]	24 h	98 ± 1	96 ± 1*	93 ± 1**	90 ± 1**
Viability (%)	48 h	95 ± 2	100 ± 2	95 ± 3	87 ± 2*
Measured interference (%)		96 ± 3	90 ± 6	85 ± 7	76 ± 6
Au[(Gly-Tyr-Met)₂B]	24 h	96 ± 1	96 ± 1*	96 ± 1*	91 ± 2**^a
Viability (%)	48 h	94 ± 1	91 ± 6*	81 ± 6**	71 ± 5**^a
Measured interference (%)		95 ± 2	92 ± 2	90 ± 4	88 ± 4
Au[(Met)₂B]	24 h	97 ± 1	96 ± 0.4*	93 ± 0.4**	94 ± 2**^a
Viability (%)	48 h	97 ± 1	91* ± 3	88 ± 4**	68 ± 4 **^a
Measured interference (%)		93 ± 1	91±	91 ± 2	89 ± 5
Au[(TrCys)₂B]	24 h	98 ± 1	97 ± 1	92 ±2*	88 ± 1**
Viability (%)	48 h	94 ± 4	93 ± 1	88 ± 2 **	77 ± 1**
Measured interference (%)		95 ± 1	93±	91 ± 3	87 ± 4

Also shown are the measured interferences in percent (%) of the control. Average values of three independent measurements are presented (mean ± SEM). Bold emphasis is used to signal the most stable AuNP; *P < 0.05 and **P < 0.01, significant differences from control values. ^aSignificant differences between response to 24- and 48-h exposure.