Table 1 Relevant parameters
Parameters | Value | ||||
|---|---|---|---|---|---|
Channel total length, Lt | 30 mm | ||||
Channel test section length, Ls | 0.66 mm | ||||
Channel contraction length, Lm | 0.2 mm | ||||
Channel main width, Wm | 0.4 mm | ||||
Channel contraction width, Wc | 0.05 mm | ||||
Channel depth, H | 0.1 mm | ||||
Channel hydraulic diameter, Dh | 66.67 ~ 160 μm | ||||
Channel contraction ratio | 8:1 | ||||
Channel expansion ratio | 1:8 | ||||
Electric field (kV/m), Ex | 5, 7.5, 10 | ||||
DNA concentration, μg/ml | 0.065 | ||||
Working fluid | 1x TBE | ||||
Viscosity (cP), μ | 1 cP | ||||
Reynolds number, Re | 0.032 ~ 0.064 | ||||
λ-DNA contour length (μm) (labeled with YOYO-1) | 21 | ||||
Radius of λ-DNA gyration (μm) | 0.7 | ||||
Temperature ( C), T | 25 | 35 | 45 | 55 | |
Relaxation time (s), τr (Rouse model) | 0.0456 | 0.0441 | 0.0427 | 0.0414 | |
Relaxation time (s), τe (Experiment) | 0.6 | ||||
Deborah number | 1.2 ~ 2.3 | ||||
Velocity vector distribution |
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